LOCUS Exported 946 bp ds-DNA linear SYN 28-MAY-2021 DEFINITION 3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation. ACCESSION . VERSION . KEYWORDS pLenti-CaMKIIa-ChETA-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 946) AUTHORS Gunaydin LA, Yizhar O, Berndt A, Sohal VS, Deisseroth K, Hegemann P TITLE Ultrafast optogenetic control. JOURNAL Nat Neurosci. 2010 Mar . 13(3):387-92. PUBMED 20081849 REFERENCE 2 (bases 1 to 946) AUTHORS . TITLE Direct Submission JOURNAL Exported May 28, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..946 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 10..19 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ccttgctcac catggtggcg gccgctggca cggctccggc ctcggcttcg tcttcgacga 61 gagtctcgac ctcgatctcc gttccgccga tgttcagttt ggtggttttg cggatatctc 121 cgtgaatcaa tatgtgctcg tggatcagga cgcgcaggta gtgtcccaac aacccccaac 181 aatttttact catcagatca ataatcgtgt gacctacggt ggagccatag acgctcagga 241 cgccaaaacc ttcgggcccc aaaatgaaga gaattgggaa cataccccag ctcacgaaaa 301 acagccatgc catgccggtc acgacctggc ggcaccgacc ctttggcaca gtatgataac 361 cctcgatata tgctttggcg gcgtgaaaaa atgtgttcgc gccatagcac aatccaagac 421 aaaagaagat gactttaaca tagccggttg ccatggcgct ggtagccccc cacacgatag 481 tcccgatgtc tgagacaagg agtcccatgg ttctcctgct gtagtcgttg ctcaggccgg 541 tgaggttgct caggcggata aggatgacag gacaagtgag cagccaggtt gcatagcgca 601 gccactgcac ccggtgtcct gtggcaaggt agagcataga gggattctta aactcaaaaa 661 agaactcgag aatcacctta accatttcaa tggcgcacac atagatctcc tcccagccgc 721 atgtagattt ccaggtttgg taggcataga acatcagcag caaaatgctg aatcctgctg 781 caagccactg caggacattt gacgcggtct gagcgccgtt cgtgccgcga gattcaatcc 841 atccggcaca gtaacattga tcctcaggga ccaggacgga cccgttcacc accacaggat 901 tagtaacgaa caaaagttcg cgtccgacgg cagacaaagc gccgcc //