LOCUS Exported 2243 bp ds-DNA circular SYN 29-JUN-2021 DEFINITION Constitutive expression of sgRNA without donor DNA. ACCESSION . VERSION . KEYWORDS pTarget12f1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2243) TITLE Genome editing by miniature CRISPR/Cas12f1 enzyme in Escherichia coli JOURNAL Unpublished REFERENCE 2 (bases 1 to 2243) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2243 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 98..115 /label=L4440 /note="L4440 vector, forward primer" promoter 192..226 /label=J23119(SpeI) promoter /note="bacterial promoter (Registry of Standard Biological Parts BBa_J23119) modified to end with an SpeI site" CDS 634..1425 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1599..2187 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2088..2107 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 121 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat 181 atgctggatc cttgacagct agctcagtcc taggtataat actagtcttc actgataaag 241 tggagaaccg cttcaccaaa agctgtccct taggggatta gaacttgagt gaaggtgggc 301 tgcttgcatc agcctaatgt cgagaagtgc tttcttcgga aagtaaccct cgaaacaaat 361 tcatttttcc tctccaattc tgcacaagaa agttgcagaa cccgaataga cgaatgaagg 421 aatgcaactg caccgtgtct agctagcttt tttttgaatt ctctagagtc gacctgcaga 481 agcttagatc tattaccctg ttatccctac tcgagttcat gtgcagctcc ataagcaaaa 541 ggggatgata agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat 601 cgactgatgt catcagcggt ggagtgcaat gtcatgaggg aagcggtgat cgccgaagta 661 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 721 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 781 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 841 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 901 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 961 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 1021 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 1081 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 1141 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 1201 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 1261 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag 1321 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt 1381 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataagatgc cgctcgccag 1441 tcgattggct gagctcataa gttcctattc cgaagttccg cgaacgcgta aaggatctag 1501 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 1561 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 1621 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 1681 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 1741 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 1801 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 1861 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 1921 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 1981 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 2041 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 2101 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 2161 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 2221 gccttttgct ggccttttgc tca //