LOCUS       Exported               12640 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral vector for NPC1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-NPC1(D786N)-FLAG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Davis OB, Shin HR, Lim CY, Wu EY, Kukurugya M, Maher CF, Perera RM, 
            Ordonez MP, Zoncu R
  TITLE     NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle 
            Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.
  JOURNAL   Dev Cell. 2020 Dec 7. pii: S1534-5807(20)30925-4. doi: 
            10.1016/j.devcel.2020.11.016.
  PUBMED    33308480
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12640
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     46..65
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             134..733
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(134..153)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     630..650
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    747..1335
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(800..820)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1218..1229)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1542..2175
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2304..2320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2304..2320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2317..2339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2328..2344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2352..2382)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2397..2418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2535..2552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2706..3294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2786..2805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3465..4325)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4088..4107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4326..4430)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4478..4612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4515..4534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4569..4588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4780..5413
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5460..5585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6082..6315
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6500..6544
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6807..6922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7117..8298
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7347..8289
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8246..8266
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             12201..12224
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        12254..113
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12289..12312)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
       61 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
      121 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      181 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      241 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      301 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
      361 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
      421 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
      481 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
      541 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
      601 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
      661 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
      721 gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
      781 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
      841 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
      901 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
      961 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     1021 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     1081 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     1141 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     1201 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     1261 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     1321 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     1381 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     1441 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     1501 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     1561 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     1621 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     1681 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     1741 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     1801 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     1861 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     1921 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     1981 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2041 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2101 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2161 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     2221 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct
     2281 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4441 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
     4501 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4561 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     4621 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     4681 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     4741 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     4801 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     4861 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     4921 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     4981 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     5041 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     5101 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     5161 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     5221 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5281 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5341 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5401 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     5461 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     5521 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     5581 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     5641 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     5701 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     5761 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     5821 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     5881 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     5941 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     6001 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     6061 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     6121 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     6181 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     6241 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     6301 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     6361 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     6421 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     6481 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     6541 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     6601 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     6661 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     6721 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     6781 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
     6841 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     6901 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
     6961 gatgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt
     7021 aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct
     7081 ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag
     7141 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     7201 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     7261 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     7321 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     7381 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg gctgcagtac
     7441 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     7501 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ttgggcgctg gggccgccgc
     7561 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     7621 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     7681 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     7741 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     7801 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     7861 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     7921 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     7981 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     8041 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     8101 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     8161 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     8221 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     8281 ccatttcagg tgtcgtgagg atcgctagcg ctaccggact cagatctcga gctcaagctt
     8341 cgaattggat ccacgtggaa ttcgccacca tgaccgctag agggctggct ctggggctgc
     8401 tgctgctgct gtgccccgct caggtgttca gtcagtcttg cgtgtggtat ggcgagtgcg
     8461 gcatcgccta tggggacaag aggtataact gtgaatactc tggaccccct aagccactgc
     8521 ccaaagacgg ctacgatctg gtgcaggaac tgtgcccagg attctttttc ggcaacgtga
     8581 gcctgtgctg tgacgtgcgg cagctgcaga ccctgaagga taatctgcag ctgcctctgc
     8641 agtttctgtc caggtgccca tcttgttttt ataacctgct gaatctgttc tgcgagctga
     8701 cctgtagtcc aaggcagtca cagttcctga acgtgactgc caccgaggac tacgtcgatc
     8761 ccgtgacaaa ccagacaaag actaatgtca aagaactgca gtactatgtg ggccagtcct
     8821 tcgccaacgc tatgtataat gcttgccgag acgtggaggc accaagctcc aatgataagg
     8881 ccctggggct gctgtgcgga aaagacgcag atgcctgtaa cgctaccaat tggatcgaat
     8941 acatgtttaa caaggataat ggccaggccc ctttcaccat tacaccagtg tttagcgact
     9001 tccccgtcca cgggatggag cctatgaaca atgccactaa gggatgcgac gagtccgtcg
     9061 atgaagtgac cgctccttgc tcctgtcagg attgctctat cgtgtgcggg cctaaaccac
     9121 agccaccacc tccaccagca ccatggacaa ttctgggact ggacgccatg tatgtgatta
     9181 tgtggatcac ttacatggcc tttctgctgg tctttttcgg cgcatttttc gccgtgtggt
     9241 gttatcgaaa gcggtacttt gtgagcgagt acacccccat tgactccaac atcgctttca
     9301 gcgtgaatgc atccgacaaa ggcgaggcat cctgctgtga tcccgtgagc gccgctttcg
     9361 aagggtgcct gcggagactg tttactcgat gggggtcttt ctgcgtgcgg aaccccggat
     9421 gcgtgatctt cttcagcctg gtgtttatca ccgcctgttc tagtgggctg gtgttcgtcc
     9481 gagtgaccac aaatccagtg gatctgtgga gcgccccatc aagccaggct cgactggaga
     9541 aggaatactt tgaccagcat ttcggaccat ttttcagaac agagcagctg atcattaggg
     9601 cacccctgac tgataaacac atctaccagc cctatccttc tggagccgac gtgcctttcg
     9661 gccctccact ggatatccag attctgcatc aggtgctgga cctgcagatc gccattgaga
     9721 acattaccgc tagctatgac aatgaaactg tgaccctgca ggatatctgc ctggctcctc
     9781 tgagcccata taacactaat tgtaccattc tgtccgtcct gaactacttt cagaatagcc
     9841 actccgtgct ggatcataag aaaggcgacg acttcttcgt gtacgccgac tatcacaccc
     9901 atttcctgta ctgcgtgcgg gctcctgcaa gtctgaacga cacctcactg ctgcacgatc
     9961 catgtctggg gacatttggc ggacccgtgt tcccttggct ggtcctggga ggctatgacg
    10021 atcagaacta caacaatgcc accgccctgg tcatcacctt ccctgtcaac aattactata
    10081 atgataccga gaagctgcag agagcacagg cctgggagaa agagttcatc aacttcgtga
    10141 agaattacaa aaacccaaat ctgaccatta gtttcacagc cgagcggagc atcgaggacg
    10201 aactgaaccg agaatctgac agtgacgtgt tcaccgtggt cattagctat gctatcatgt
    10261 tcctgtacat ctcactggca ctgggccata tgaagagctg caggcgcctg ctggtggatt
    10321 caaaagtcag cctgggaatt gccggcatcc tgattgtgct gtcctctgtc gcttgtagcc
    10381 tgggggtgtt ctcctatatt ggactgcctc tgaccctgat cgtcattgaa gtgatcccat
    10441 ttctggtcct ggctgtcgga gtggacaata tcttcattct ggtgcaggca taccagcgcg
    10501 acgagcgact gcagggcgaa acactggatc agcagctggg aagggtgctg ggagaggtcg
    10561 ctccatctat gtttctgagt tcattcagtg aaaccgtggc atttttcctg ggcgccctga
    10621 gtgtgatgcc agcagtccac acattttcac tgttcgctgg gctggcagtg tttattgact
    10681 tcctgctgca gatcacctgc tttgtgagcc tgctgggcct gaacattaag aggcaggaga
    10741 aaaaccgcct ggatatcttc tgctgcgtgc ggggagcaga ggacggcaca tccgtgcagg
    10801 cttcagaaag ctgtctgttc aggtttttca agaacagcta ttctccactg ctgctgaaag
    10861 actggatgcg ccccatcgtg attgcaatct ttgtcggagt gctgagtttc tcaattgccg
    10921 tgctgaacaa ggtcgacatc ggcctggatc agagcctgtc catgcccgac gattcttaca
    10981 tggtggatta ctttaaatct atcagtcagt atctgcacgc cggcccccct gtctacttcg
    11041 tgctggagga aggccatgac tacactagct ccaaggggca gaatatggtg tgcgggggaa
    11101 tgggatgtaa caatgattcc ctggtgcagc agatcttcaa cgcagcccag ctggacaatt
    11161 atacaaggat tggctttgcc ccctctagtt ggatcgacga ttacttcgat tgggtcaagc
    11221 ctcagtcaag ctgctgtcgc gtggacaaca tcacagatca gttttgcaat gcaagcgtgg
    11281 tcgaccctgc ctgcgtgaga tgtaggccac tgactccaga gggcaaacag cgaccacagg
    11341 gaggcgactt catgagattc ctgcctatgt tcctgtccga caacccaaat cccaagtgtg
    11401 gcaaaggagg ccacgctgca tactcctctg cagtcaacat cctgctggga catgggaccc
    11461 gagtgggagc cacatacttc atgacatacc acactgtgct gcagacctca gctgacttca
    11521 ttgatgcact gaagaaagcc cgcctgatcg ctagcaacgt gacagagact atggggatca
    11581 atggatccgc ctatcgagtg tttccctact ctgtctttta cgtgttctat gaacagtacc
    11641 tgactatcat tgacgatacc atcttcaacc tgggggtgtc cctgggagct atcttcctgg
    11701 tcactatggt gctgctgggc tgcgagctgt ggtctgccgt gattatgtgc gccaccatcg
    11761 ctatggtcct ggtgaatatg ttcggcgtga tgtggctgtg ggggatctct ctgaacgccg
    11821 tcagtctggt gaatctggtc atgtcttgcg ggattagtgt ggagttttgt agccatatca
    11881 ctagagcttt caccgtctca atgaagggaa gccgggtgga aagagcagag gaagctctgg
    11941 cacacatggg aagttcagtg tttagtggca ttaccctgac aaagttcggg ggaatcgtgg
    12001 tcctggcctt tgctaagagc cagatcttcc agatctttta tttccggatg tacctggcta
    12061 tggtgctgct gggcgcaaca cacgggctga ttttcctgcc cgtcctgctg tcatatatcg
    12121 gccctagcgt gaacaaggca aaaagctgcg ccactgagga aagatacaag gggactgagc
    12181 gagagagact gctgaacttt gactacaagg atgacgatga taaataaccc gggctcatct
    12241 agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
    12301 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
    12361 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
    12421 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
    12481 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
    12541 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
    12601 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
//
