LOCUS Exported 5758 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988. ACCESSION . VERSION . KEYWORDS pSynSRE-Mut-T-Luc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5758) AUTHORS Smith JR, Osborne TF, Brown MS, Goldstein JL, Gil G TITLE Multiple sterol regulatory elements in promoter for hamster 3-hydroxy-3-methylglutaryl-coenzyme A synthase. JOURNAL J Biol Chem. 1988 Dec 5;263(34):18480-7. PUBMED 2903862 REFERENCE 2 (bases 1 to 5758) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5758 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 1..122 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS 383..2035 /codon_start=1 /gene="luc" /product="firefly luciferase" /label=luciferase /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" primer_bind complement(449..467) /label=LucNrev /note="Firefly luciferase, reverse primer" intron 2276..2341 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2471..2491 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 2916..3050 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3192..3209) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3363..3951) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3443..3462) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4122..4982) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4745..4764 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4983..5087) /gene="bla" /label=AmpR promoter rep_origin 5114..5569 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5201..5220) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5411..5432 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 61 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 121 tatggtactg taactgagct aacataaccc gggaggtacc gagctctggc tctcgccgtc 181 tctcaccctc cccccattgg ccgagatgtt ctcgggcccc cctccgtcac cgtctcaccc 241 cacttccccc gtcctccgac tttgtcgcta gctcgacaag gtcaactata aagctgctga 301 gggcgggagg cacagtgcac tgtgctccgt ggattgctcc tttctttgcc cccaagcttg 361 gcattccggt actgttggta aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc 421 attctatcct ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata 481 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta 541 cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa 601 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt 661 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga 721 attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt 781 gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga 841 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc 901 tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc 961 actgataatg aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag 1021 aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc 1081 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact 1141 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt 1201 tttacgatcc cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc 1261 attcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc 1321 ttctgggggc gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct 1381 tccagggata cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc 1441 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt 1501 tgtggatctg gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag 1561 aggacctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga 1621 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt 1681 catagttgac cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga 1741 attggaatcg atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc 1801 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat 1861 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg 1921 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag 1981 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaaatgt 2041 aactgtattc agcgatgacg aaattcttag ctattgtaat actgcgatga gtggcagggc 2101 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga 2161 ataagtgata ataagcggat gaatggcaga aattcgccgg atctttgtga aggaacctta 2221 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa 2281 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta 2341 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa 2401 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca 2461 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt 2521 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta 2581 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac 2641 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag 2701 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat 2761 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca 2821 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 2881 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 2941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 3001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgtcgaccga 3061 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg 3121 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc 3181 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 3241 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 3301 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 3361 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 3421 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 3481 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 3541 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 3601 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 3661 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 3721 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 3781 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 3841 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 3901 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 3961 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 4021 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 4081 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 4141 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 4201 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 4261 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 4321 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 4381 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 4441 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 4501 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 4561 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 4621 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 4681 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 4741 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 4801 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 4861 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 4921 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 4981 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 5041 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 5101 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 5161 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 5221 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 5281 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 5341 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 5401 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 5461 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 5521 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca 5581 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 5641 cccaagctac catgataagt aagtaatatt aaggtacgtg gaggttttac ttgctttaaa 5701 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgtt //