LOCUS       Exported                 834 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Expresses sfGFP with SpyTag003 and flanking linkers between residues
            between residues Val22 and Asn23 in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    pET28-sfGFP-SpyTag003 Loop A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 834)
  TITLE     DogCatcher allows Loop-friendly Protein-Protein Ligation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             811..828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
       61 gatgtcggcg ggggtggctc gcgtggtgtt ccacatatcg ttatggttga tgcgtacaaa
      121 cgctataagg gaggaggtgg ctctaacggt cataagtttt ccgtgcgtgg cgagggtgaa
      181 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
      241 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
      301 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
      361 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
      421 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
      481 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
      541 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
      601 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
      661 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
      721 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
      781 gaactgtaca aaggctccgg ctccggctcc caccatcacc atcaccattg ataa
//
