LOCUS Exported 9377 bp ds-DNA circular SYN 26-MAY-2021 DEFINITION 3rd generation lentiviral vector. ACCESSION . VERSION . KEYWORDS pLX304 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9377) AUTHORS Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE TITLE A public genome-scale lentiviral expression library of human ORFs. JOURNAL Nat Methods. 2011 Jun 26. ():. PUBMED 21706014 REFERENCE 2 (bases 1 to 9377) AUTHORS . TITLE Direct Submission JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9377 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 66..369 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 370..573 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 524..544 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 596..720 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 745..775 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 829..1488 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(895..914) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 1830..2135 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2014..2033 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2176..2300) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 2302..2343 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2385..2973 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2438..2458) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2856..2867) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3045..3278 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3356..3477 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3393..3412) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3447..3466 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3517..3652 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3579..3598 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(3672..3691) /label=T7 /note="T7 promoter, forward primer" promoter complement(3673..3691) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3701..3718) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3701..3717) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3710..3732) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3859..4314 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3946..3965) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4156..4177 /label=F1ori-F /note="F1 origin, forward primer" promoter 4340..4444 /gene="bla" /label=AmpR promoter CDS 4445..5305 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4663..4682) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5476..6064 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5965..5984 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6218..6235 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6352..6373 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6388..6418 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6426..6442 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6431..6453 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6450..6466 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6450..6466 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6485..6505 /label=T3 /note="T3 promoter, forward primer" promoter 6487..6505 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6533..6759 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 6760..6940 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 6987..7112 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7605..7838 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8023..8067 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8242..8742 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 8661..8679 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 8754..9149 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 9209..9326 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag 601 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta 661 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg 721 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg 781 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa 841 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca 901 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt 961 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc 1021 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata 1081 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg 1141 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg 1201 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc 1261 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac 1321 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg 1381 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg 1441 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc 1501 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga 1561 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta 1621 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat 1681 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg 1741 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc 1801 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag 1861 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac 1921 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt 1981 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg 2041 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca 2101 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc 2161 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt 2221 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca 2281 gctttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct 2341 acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt 2401 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 2461 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 2521 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 2581 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 2641 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 2701 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 2761 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 2821 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 2881 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 2941 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa 3001 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 3061 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 3121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 3181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 3241 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca 3301 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 3361 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 3421 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 3481 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 3541 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 3601 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg 3661 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac 3721 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 3781 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 3841 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3901 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 3961 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 4021 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 4081 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 4141 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 4201 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 4261 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg 4321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 4381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 4441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 4501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 4561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 4621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 4681 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 4741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 4801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 4861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 4921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 4981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 5041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 5101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 5161 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 5221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 5281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 5341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 5401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 5461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 5521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 5581 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 5641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 5701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 5761 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 5821 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 5881 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 5941 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 6001 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 6061 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 6121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 6181 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 6241 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6301 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 6361 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 6421 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 6481 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct 6541 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 6601 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 6661 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 6721 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 6781 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 6841 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 6901 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 6961 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 7021 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 7081 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 7141 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 7201 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 7261 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 7321 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 7381 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 7441 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga 7501 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag 7561 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt 7621 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag 7681 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag 7741 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc 7801 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga 7861 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa 7921 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc 7981 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta 8041 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg 8101 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct 8161 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac 8221 ctcccaaccc cgaggggacc cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga 8281 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca 8341 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg 8401 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg 8461 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga 8521 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc 8581 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg 8641 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca 8701 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggtacc accatggcca 8761 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca 8821 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct 8881 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg 8941 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga 9001 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg 9061 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat 9121 tgctgccctc tggttatgtg tgggagggcc tgcagctgca gtagtaagaa ttctagatct 9181 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca 9241 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa 9301 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt 9361 ggcgccggct cgagggg //