LOCUS Exported 11571 bp ds-DNA circular SYN 03-JUN-2021 DEFINITION Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells. ACCESSION . VERSION . KEYWORDS pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11571) AUTHORS Bertolin AP, Weissmann F, Zeng J, Posse V, Milligan JC, Canal B, Ulferts R, Wu M, Drury LS, Howell M, Beale R, Diffley JFX TITLE Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp12/7/8 RNA-dependent RNA Polymerase JOURNAL bioRxiv https://www.biorxiv.org/content/10.1101/2021.04.07.438807v1 REFERENCE 2 (bases 1 to 11571) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11571 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 126..174 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" mobile_element 452..617 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" promoter 1002..1104 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1105..1764 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1171..1190) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 2064..2518 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2151..2170) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2360..2381 /label=F1ori-F /note="F1 origin, forward primer" promoter 2544..2648 /gene="bla" /label=AmpR promoter CDS 2649..3509 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2867..2886) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3680..4268 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4169..4188 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4422..4439 /label=L4440 /note="L4440 vector, forward primer" mobile_element 4573..4797 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(4864..5397) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(5586..5614) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" protein_bind 6010..6043 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 6141..6189 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter 6284..6375 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 9233..9298 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" polyA_signal 9431..9565 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 9924..9972 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter 10067..10158 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 10460..10477 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 11204..11338 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 cgtcggggcg gcaagccctg ccatagccac tacgggttaa ttaaaaactg gatactattg 61 cacgttttta tcgaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 121 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac 181 gcggggttcg gtcccagggc tggcactctg tcaagtgaac gctctatggt ctaaagattt 241 aaatctatat ctcaatcggg gttacgtcga taccccaccg agaccccatt gggaccaata 301 cgcccgcgtt tcttcctttt ccccacccca acccccaagt tcgggtgaag gcccagggct 361 cgcagccaac gtcggggcgg caagccctgc catagccact acgggttaat taaaaaccta 421 atgatgcctg atgtttcaat caggagatcc gaaccagata agtgaaatct agttccaaac 481 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt 541 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac 601 tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct 661 gccaactcca tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa 721 tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg 781 cagcctgaac tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa 841 aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa 901 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat 961 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt 1021 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga 1081 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat 1141 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc 1201 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag 1261 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa 1321 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 1381 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 1441 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 1501 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 1561 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 1621 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 1681 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 1741 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct 1801 ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcaaat 1861 tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt 1921 ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc 1981 agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt tattctgcct 2041 cccagagcct gatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 2101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 2161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 2221 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 2281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 2341 ggagtccacg ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 2401 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 2461 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 2521 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2581 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2641 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 2701 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 2761 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 2821 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 2881 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 2941 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 3001 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 3061 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 3121 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 3181 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 3241 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 3301 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 3361 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 3421 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 3481 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 3541 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 3601 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 3661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 3721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 3781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactattctt ctagtgtagc 3841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 3901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 3961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 4021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 4081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 4141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 4201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 4261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 4321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 4381 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 4441 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 4501 gcatagacca gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc 4561 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta 4621 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct 4681 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc 4741 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg 4801 gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa 4861 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa 4921 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata 4981 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg 5041 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc 5101 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc 5161 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg 5221 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat 5281 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc 5341 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt 5401 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc 5461 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt 5521 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc 5581 attacagttt acgaaccgaa caggcttatg tcaactgggt ttgtgccttc atccgtttcc 5641 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc 5701 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt 5761 tcttctacgg caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc 5821 ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt 5881 ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc 5941 tacagctttg tttgtactat caacaggttg aactgctgat caacagatcc tctacgcggc 6001 cgcggtacca taacttcgta tagcatacat tatacgaagt tatctggcct aggttaatta 6061 aacgctctat ggtctaaagt ttttatcgag gctaactgaa acacggaagg agacaatacc 6121 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt 6181 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcaag tgaacgctct 6241 atggtctaaa gatttaaatc gacctactcc ggaatattaa tagatcatgg agataattaa 6301 aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata 6361 aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg cggatccagc 6421 caccatgtct gctgacgctc agtccttcct gaaccgtgtg tgcggtgtct ctgctgctcg 6481 tctgactcct tgcggtactg gaacctctac cgacgtggtg taccgtgctt tcgacatcta 6541 caacgacaag gtggccggtt tcgctaagtt cctcaagacc aactgctgcc gcttccaaga 6601 gaaggacgag gacgacaacc tgatcgactc ctacttcgtg gtcaagcgtc acaccttctc 6661 caactaccag cacgaggaaa ctatctacaa cctgctgaag gactgccccg ctgtggctaa 6721 gcacgacttc ttcaagttcc gtatcgacgg cgacatggtg ccccacatct ctcgtcagcg 6781 tctgaccaag tacaccatgg ctgacctggt gtacgctctg cgccacttcg atgagggaaa 6841 ctgcgacacc ctgaaagaga tcctggtcac ctacaactgc tgtgacgacg actacttcaa 6901 caagaaggat tggtacgact tcgtcgagaa ccccgacatc ctgcgtgtgt acgctaacct 6961 gggagagcgt gtgcgtcagg ctctgctcaa gactgtgcag ttctgcgacg ctatgcgtaa 7021 cgctggtatc gtgggagtgc tgaccctgga caaccaggac ctgaacggca attggtatga 7081 tttcggcgac ttcatccaga ctacccctgg ttctggtgtc cccgtggtgg actcttacta 7141 ctccctgctg atgcccatcc tgactctgac ccgtgctctg accgctgagt ctcacgtgga 7201 caccgacctg accaagcctt acatcaagtg ggacttgctg aagtacgatt tcaccgagga 7261 acgcctgaag ctgttcgacc gttacttcaa gtactgggac cagacctacc atcctaactg 7321 cgtgaactgc ctggacgacc gttgcatcct gcactgcgct aacttcaacg tgctgttctc 7381 cactgtgttc cctccaacct ccttcggtcc tctcgtgcgc aagatcttcg tggacggtgt 7441 ccctttcgtg gtgtccaccg gttaccactt ccgtgaactg ggtgtcgtgc ataaccagga 7501 cgtcaacctc cactcctctc gtctgtcctt caaagagctg ctggtctacg ctgctgaccc 7561 cgctatgcat gctgcttccg gaaacctgct gctggacaag cgtaccactt gcttctccgt 7621 ggctgccctg actaacaacg tggccttcca gaccgtgaag cccggcaatt tcaacaagga 7681 cttctacgat ttcgccgtgt ccaagggctt cttcaaagaa ggctcctccg tcgagctgaa 7741 gcacttcttc ttcgctcaag acggcaacgc tgctatctcc gactacgact actaccgtta 7801 caacttgccc accatgtgcg acatccgtca gctgctgttc gtggtcgagg tggtggacaa 7861 gtacttcgac tgctacgacg gtggttgcat caacgccaat caagtgatcg tgaacaacct 7921 ggacaagtcc gctggcttcc ccttcaacaa atggggcaaa gctcgtctgt actacgactc 7981 catgtcctac gaggaccagg acgctctgtt cgcttacacc aagcgtaacg tgatccccac 8041 catcactcag atgaacctga aatacgccat ctccgctaag aaccgtgctc gtaccgtggc 8101 tggtgtctcc atctgctcca ccatgaccaa ccgtcagttc caccagaagc tgctgaagtc 8161 aatcgctgct acccgcggtg ctaccgtggt catcggtact tccaagttct acggtggctg 8221 gcacaacatg ctgaaaaccg tgtactccga cgtggaaaac cctcacctga tgggctggga 8281 ctaccccaag tgcgatcgtg ctatgcctaa catgctgcgt atcatggctt ccctggtgct 8341 ggctcgcaag cacactactt gctgctccct gtctcaccgt ttctaccgtc tggctaacga 8401 gtgcgctcag gtgctgtccg agatggtcat gtgcggtggt tccctgtacg tgaagcctgg 8461 tggtacttcc tctggcgacg ctaccaccgc ttacgctaac tccgtgttca acatctgcca 8521 ggctgtgacc gctaacgtga acgctctgct gtccaccgac ggcaacaaga tcgctgacaa 8581 atacgtgcgt aacctgcagc accgtctgta cgagtgcctg taccgtaacc gtgacgtcga 8641 cactgacttc gtgaacgagt tctacgctta cctgcgcaag cacttcagca tgatgatcct 8701 gtccgacgac gctgtcgtgt gcttcaactc cacctacgct tcccaaggcc tggtggcttc 8761 catcaagaac ttcaagtccg tgctgtacta ccagaacaac gtgttcatgt ccgaggctaa 8821 gtgctggacc gagactgacc tgactaaggg tccccacgag ttctgctccc agcacaccat 8881 gctggtcaag cagggcgacg attacgtgta cctgccttat cctgatcctt ctcgtatcct 8941 cggcgctggt tgcttcgtgg atgacatcgt caagaccgac ggaaccctga tgatcgagcg 9001 tttcgtgtcc ctggctatcg acgcttaccc tctgactaag caccccaatc aagagtacgc 9061 tgacgtgttc cacctgtacc tgcagtacat ccgcaagctg cacgacgagc tgaccggaca 9121 catgctggac atgtactccg tgatgctgac caacgacaac acctctcgtt actgggagcc 9181 cgaattctac gaggctatgt acacccctca caccgtgctg cagggtggct ccgactacaa 9241 ggaccacgac ggcgattaca aggatcatga catcgactac aaagacgacg atgacaagta 9301 aaagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt 9361 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 9421 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 9481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 9541 caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta 9601 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac 9661 catttaaata aatctatatc tcaatcgggg ttacgtcgat accccaccga gaccccattg 9721 ggaccaatac gcccgcgttt cttccttttc cccaccccaa cccccaagtt cgggtgaagg 9781 cccagggctc gcagccaacg tcggggcggc aagccctgcc atagccacta cgggttaatt 9841 aaaaactata tctcaatcgg ggtttttatc gaggctaact gaaacacgga aggagacaat 9901 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg 9961 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc aagtgaacgc 10021 tctatggtct aaagatttaa atcgacctac tccggaatat taatagatca tggagataat 10081 taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta 10141 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatcc 10201 agccaccatg tccaagatgt ccgacgtgaa gtgcacctcc gtggtgctgc tgtctgtgct 10261 gcaacagctg cgtgtggaat cctccagcaa gctgtgggct cagtgcgtcc aactgcacaa 10321 cgacatcctg ctggctaagg acaccaccga ggctttcgag aagatggtgt ccctgctgtc 10381 cgtcctgctg tctatgcaag gtgctgtgga catcaacaag ctgtgcgaag agatgctgga 10441 caaccgtgct accctgcagc accatcacca ccatcacgct atcgcttccg agttctcctc 10501 tctgccctcc tacgctgctt tcgctaccgc tcaagaggct tacgagcagg ctgtggctaa 10561 cggcgactct gaggtggtgc tgaagaagct gaaaaagtcc ctgaacgtgg ccaagtccga 10621 gttcgaccgt gacgctgcta tgcagcgcaa gctcgagaag atggctgacc aggctatgac 10681 ccagatgtac aagcaggctc gttccgagga caagcgtgct aaagtgacct ccgctatgca 10741 gaccatgctg ttcaccatgc tccgcaagct ggacaacgac gctctgaaca acatcatcaa 10801 caacgctcgt gacggttgcg tgcccctgaa catcatccct ctgaccaccg ctgctaagct 10861 gatggtggtc atccccgact acaacaccta caagaacact tgcgacggca ccaccttcac 10921 ctacgcttcc gctctgtggg agatccagca agtggtggac gctgactcca agatcgtgca 10981 gctgtccgag atctccatgg acaactctcc caacctggct tggcccctga tcgtgactgc 11041 tctgcgtgct aactccgctg tgaagctgca gtaaaagctt gtcgagaagt actagaggat 11101 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 11161 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 11221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 11281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg 11341 atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg 11401 ctgctgccac cgctgagcaa taactatcat aaccatttaa ataaatctat atctcaatcg 11461 gggttacgtc gataccccac cgagacccca ttgggaccaa tacgcccgcg tttcttcctt 11521 ttccccaccc caacccccaa gttcgggtga aggcccaggg ctcgcagcca a //