LOCUS Exported 5912 bp ds-DNA circular SYN 08-JUN-2021 DEFINITION Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized). ACCESSION . VERSION . KEYWORDS pCAG-NLS-HA-Dre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5912) AUTHORS Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P TITLE Binary recombinase systems for high-resolution conditional mutagenesis. JOURNAL Nucleic Acids Res. 2014 Jan 9. PUBMED 24413561 REFERENCE 2 (bases 1 to 5912) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 8, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5912 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 7..386 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 388..665 /label=chicken beta-actin promoter intron 666..1682 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1606..1628 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1695..1714 /label=T7 /note="T7 promoter, forward primer" promoter 1695..1713 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1733..1753 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1754..1780 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(2832..2849) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2838..3062 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(3134..3151) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3134..3150) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3143..3165) /label=M13/pUC Forward /note="In lacZ gene" rep_origin complement(3291..3746) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3428..3449) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3640..3659 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3773..3877 /gene="bla" /label=AmpR promoter CDS 3878..4738 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4096..4115) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4909..5497 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5398..5417 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5651..5668 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5785..5806 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5821..5851 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5859..5875 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5864..5886 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 5883..5899 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 5883..5899 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 ggattcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 361 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc 421 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg 481 cagcgatggg ggcggggggg gggggggggc gcgcgccrgg sggggsgggg sggggsgrgg 541 ggsggggsgg ggsgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 661 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc 721 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt 781 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc 841 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg 901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg 961 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg 1021 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg 1081 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc 1141 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg 1201 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg 1261 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg 1321 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 1381 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 1441 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 1561 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 1621 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 1681 agatccttaa ttaataatac gactcactat agggggtcga cccgccacca tgcctaagaa 1741 gaagaggaag gtttacccat acgatgttcc agattacgct ggatcctctg agctgattat 1801 tagtggttca tctggtggat tcctgcgaaa catcggcaaa gagtatcagg aggccgctga 1861 aaacttcatg aggtttatga atgaccaggg ggcgtacgct cctaacactt tgagggattt 1921 gaggttggtc tttcatagct gggccagatg gtgccatgct cggcagcttg catggtttcc 1981 aattagtcct gaaatggcac gcgaatactt tcttcagttg cacgatgcag acctggcctc 2041 cactaccatc gacaagcact atgctatgct taatatgctt ctgtcccact gcggactgcc 2101 acccttgtcc gacgacaagt cagtgagtct tgccatgaga agaattagaa gagaagccgc 2161 aaccgaaaag ggtgagagga caggacaggc aatccccctg cgctgggacg acctgaagct 2221 gctggatgtg ctgctcagca ggagcgagcg gctggtcgac ctgcgcaaca gggctttcct 2281 gttcgtagcc tataacaccc tcatgagaat gtctgaaata tcacgcatca gggttgggga 2341 cttggatcag acaggagaca cagtgaccct gcacatcagt cacactaaga caatcaccac 2401 agctgcgggc cttgacaaag tgctctcccg gcgaaccaca gcagtgctca atgactggct 2461 ggacgtcagt gggcttagag aacatccaga cgctgtgctc ttcccaccta tacaccggtc 2521 aaacaaagcc cgcattacta ccacgcccct gaccgcccct gccatggaga agattttcag 2581 tgatgcctgg gtgctgctga acaaacggga cgccaccccc aataaaggga ggtataggac 2641 ctggaccggc cattccgcca gggtgggtgc cgcaatagac atggccgaga aacaggtgtc 2701 tatggtcgag attatgcagg aagggacatg gaagaagcct gaaacactga tgcggtatct 2761 cagaaggggc ggagtgtccg tgggagccaa ttctcgactg atggatagct gagtcagagc 2821 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 2881 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 2941 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 3001 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 3061 ggcttctgag gcggaaagaa ccagctgggg ctcgagatcc actagttcta gcctcgaggc 3121 tagagcggcc gccactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 3181 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 3241 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag 3301 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 3361 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 3421 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 3481 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 3541 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 3601 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 3661 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 3721 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac 3781 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 3841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 3901 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 3961 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 4021 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 4081 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 4141 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 4201 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 4261 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 4321 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 4381 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 4441 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 4501 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 4561 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 4621 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 4681 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 4741 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 4801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 4861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 4921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 4981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 5041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 5101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 5161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 5221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 5281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 5341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 5401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 5461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 5521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 5581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 5641 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 5701 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 5761 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 5821 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 5881 cacaggaaac agctatgacc atgaggcgcg cc //