LOCUS       Exported                 937 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    Sgf11/pCDF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, 
            Wolberger C
  TITLE     Structural insights into the assembly and function of the SAGA 
            deubiquitinating module.
  JOURNAL   Science. 2010 May 21. 328(5981):1025-9.
  PUBMED    20395473
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             377..421
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(459..477)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      473..520
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggggaattc cctgtaaata ttttgtttac tttaataagg agatatacca tggctatgac
       61 cgaagaaact attactatag attctatttc aaatgggata ctgaataacc tgttaaccac
      121 attgatccag gacattgtag ctcgggaaac cactcaacaa caattgctga agacaagata
      181 tccggatctt cgcagttatt atttcgaccc gaacggatct ctcgatatta atggactaca
      241 gaagcaacaa gagtcctctc agtatattca ctgtgagaat tgtggcaggg atgtgtccgc
      301 aaacagacta gcagctcatt tacagagatg tttgagtagg ggtgctagac gttgagcggc
      361 cgcactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc
      421 gtctactagc gcagcttaat taacctaggc tgctgccacc gctgagcaat aactagcata
      481 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga
      541 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata
      601 agcggctatt taacgaccct gccctgaacc gacgaccggg tcatcgtggc cggatcttgc
      661 ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc
      721 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
      781 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
      841 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
      901 ccaaatgcgg gacaacgtaa gcactacatt tcgctct
//
