LOCUS       Exported                 925 bp ds-DNA     linear   SYN 05-JUL-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    Sus1/PRSF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, 
            Wolberger C
  TITLE     Structural insights into the assembly and function of the SAGA 
            deubiquitinating module.
  JOURNAL   Science. 2010 May 21. 328(5981):1025-9.
  PUBMED    20395473
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    407..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(431..450)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(432..450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        496..573
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(593..612)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 acgagtccat gtgctggcgt tcaatttcgc agcagcggtt tctttaccag actcgagtgc
       61 ggccgctcat tgtgtatcta caatctcttc aagaaattcc cttatttgct ttaaaacggt
      121 ttcccttgtt gaatccgata ccatttctaa tgctttgggt tctacggtgg ataaaatttg
      181 ggtaaagttc gtagattcat ttatgttcat ctctgatttg gtcaaatctt tgactttatc
      241 cacccaacct tcttgaagta gtctggcttt tagttcgttt gaaattagtt catagtttcc
      301 tgattctaca agatattgtt gtatttgact ctttaattgc gcagtatcca tagtcatagc
      361 catggtatat ctccttatta aagttaaaca aaattatttc tacaggggaa ttgttatccg
      421 ctcacaattc ccctatagtg agtcgtatta atttcctaat gcaggagtcg cataagggag
      481 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag
      541 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc
      601 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
      661 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
      721 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
      781 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
      841 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
      901 gcggtgcaca atctcccccg cacgg
//
