LOCUS Exported 11710 bp ds-DNA circular SYN 08-JUN-2021 DEFINITION mammalian expression vector. ACCESSION . VERSION . KEYWORDS pcDNA5-FRT/TO-eGFP-53BP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11710) AUTHORS Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D TITLE 53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. JOURNAL Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12. PUBMED 23760478 REFERENCE 2 (bases 1 to 11710) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 8, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11710 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 168..547 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 548..751 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 702..722 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 753..771 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 774..792 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 802..826 /label=LNCX /note="Human CMV promoter, forward primer" CDS 923..1639 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 923..1639 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(968..989) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1229..1248) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1576..1597 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(7595..7612) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7601..7825 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7958..7977) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 8109..8156 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 8164..9186 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 9316..9437 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9353..9372) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9407..9426 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9486..9502) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9486..9502) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9499..9521) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9510..9526 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9534..9564) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9579..9600 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9717..9734) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9888..10476) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9968..9987) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10647..11507) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11270..11289 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11508..11612) /gene="bla" /label=AmpR promoter primer_bind complement(11687..11706) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 61 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt 121 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt 181 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 241 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 301 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 361 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 421 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 481 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 541 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 601 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 661 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 721 tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag atctccctat 781 cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca 841 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc 901 gtttaaactt aagcttggta ccatggtgag caagggcgag gagctgttca ccggggtggt 961 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 1021 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 1081 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 1141 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 1201 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 1261 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 1321 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 1381 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 1441 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 1501 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 1561 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 1621 catggacgag ctgtacaagg gcgcgccaat ggaccctact ggaagtcagt tggattcaga 1681 tttctctcag caagatactc cttgcctgat aattgaagat tctcagcctg aaagccaggt 1741 tctagaggat gattctggtt ctcacttcag tatgctatct cgacaccttc ctaatctcca 1801 gacgcacaaa gaaaatcctg tgttggatgt tgtgtccaat cctgaacaaa cagctggaga 1861 agaacgagga gacggtaata gtgggttcaa tgaacatttg aaagaaaaca aggttgcaga 1921 ccctgtggat tcttctaact tggacacatg tggttccatc agtcaggtca ttgagcagtt 1981 acctcagcca aacaggacaa gcagtgttct gggaatgtca gtggaatctg ctcctgctgt 2041 ggaggaagag aagggagaag agttggaaca gaaggagaaa gagaaggaag aagatacttc 2101 aggcaatact acacattccc ttggtgctga agatactgcc tcatcacagt tgggttttgg 2161 ggttctggaa ctctcccaga gccaggatgt tgaggaaaat actgtgccat atgaagtgga 2221 caaagagcag ctacaatcag taaccaccaa ctctggttat accaggctgt ctgatgtgga 2281 tgctaatact gcaattaagc atgaagaaca gtccaacgaa gatatcccca tagcagaaca 2341 gtccagcaag gacatccctg tgacagcaca gcccagtaag gatgtacatg ttgtaaaaga 2401 gcaaaatcca ccacctgcaa ggtcagagga catgcctttt agccccaaag catctgttgc 2461 tgctatggaa gcaaaagaac agttgtctgc acaagaactt atggaaagtg gactgcagat 2521 tcagaagtca ccagagcctg aggttttgtc aactcaggaa gacttgtttg accagagcaa 2581 taaaacagta tcttctgatg gttgctctac tccttcaagg gaggaaggtg ggtgttcttt 2641 ggcttccact cctgccacca ctctgcatct cctgcagctc tctggtcaga ggtcccttgt 2701 tcaggacagt ctttccacga attcttcaga tcttgttgct ccttctcctg atgctttccg 2761 atctactcct tttatcgttc ctagcagtcc cacagagcaa gaagggagac aagataagcc 2821 aatggacacg tcagtgttat ctgaagaagg aggagagcct tttcagaaga aacttcaaag 2881 tggtgaacca gtggagttag aaaacccccc tctcctgcct gagtccactg tatcaccaca 2941 agcctcaaca ccaatatctc agagcacacc agtcttccct cctgggtcac ttcctatccc 3001 atcccagcct cagttttctc atgacatttt tattccttcc ccaagtctgg aagaacaatc 3061 aaatgatggg aagaaagatg gagatatgca tagttcatct ttgacagttg agtgttctaa 3121 aacttcagag attgaaccaa agaattcccc tgaggatctt gggctatctt tgacagggga 3181 ttcttgcaag ttgatgcttt ctacaagtga atatagtcag tccccaaaga tggagagctt 3241 gagttctcac agaattgatg aagatggaga aaacacacag attgaggata cggaacccat 3301 gtctccagtt ctcaattcta aatttgttcc tgctgaaaat gatagtatcc tgatgaatcc 3361 agcacaggat ggtgaagtac aactgagtca gaatgatgac aaaacaaagg gagatgatac 3421 agacaccagg gatgacatta gtattttagc cactggttgc aagggcagag aagaaacggt 3481 agcagaagat gtttgtattg atctcacttg tgattcgggg agtcaggcag ttccgtcacc 3541 agctactcga tctgaggcac tttctagtgt gttagatcag gaggaagcta tggaaattaa 3601 agaacaccat ccagaggagg ggtcttcagg gtctgaggtg gaagaaatcc ctgagacacc 3661 ttgtgaaagt caaggagagg aactcaaaga agaaaatatg gagagtgttc cgttgcacct 3721 ttctctgact gaaactcagt cccaagggtt gtgtcttcaa aaggaaatgc caaaaaaaga 3781 atgctcagaa gctatggaag ttgaaaccag tgtgattagt attgattccc ctcaaaagtt 3841 ggcaatactt gaccaagaat tggaacataa ggaacaggaa gcttgggaag aagctacttc 3901 agaggactcc agtgttgtca ttgtagatgt gaaagagcca tctcccagag ttgatgtttc 3961 ttgtgaacct ttggagggag tggagaagtg ctcagattcc cagtcatggg aggatattgc 4021 tccagaaata gaaccatgtg ctgagaatag attagacacc aaggaagaaa agagtgtaga 4081 atatgaagga gatctgaaat cagggactgc agaaacagaa cctgtagagc aagattcttc 4141 acagccttcc ttacctttag tacgcgctga cgacccgctt agacttgacc aggagttgca 4201 gcagccccaa actcaggaga aaacaagtaa ttcattaaca gaagactcaa aaatggctaa 4261 tgcaaagcag ctaagctcag atgcagaggc ccagaagctg gggaagccct ctgcccatgc 4321 ctcacaaagc ttctgtgaaa gttctagtga aaccccattt catttcactt tgcctaaaga 4381 aggtgatatc atcccaccat tgactggtgc aaccccacct cttattgggc acctaaaatt 4441 ggagcccaag agacacagta ctcctattgg tattagcaac tatccagaaa gcaccatagc 4501 aaccagtgat gtcatgtctg aaagcatggt ggagacccat gatcccatac ttgggagtgg 4561 aaaaggggat tctggggctg ccccagacgt ggatgataaa ttatgtctaa gaatgaaact 4621 ggttagtcct gagactgagg cgagtgaaga gtctttgcag ttcaacctgg aaaagcctgc 4681 aactggtgaa agaaaaaatg gatctactgc tgttgctgag tctgttgcca gtccccagaa 4741 gaccatgtct gtgttgagct gtatctgtga agccaggcaa gagaatgagg ctcgaagtga 4801 ggatcccccc accacaccca tcagggggaa cttgctccac tttccaagtt ctcaaggaga 4861 agaggagaaa gaaaaattgg agggtgacca tacaatcagg cagagtcaac agcctatgaa 4921 gcccattagt cctgtcaagg accctgtttc tcctgcttcc cagaagatgg tcatacaagg 4981 gccatccagt cctcaaggag aggcaatggt gacagatgtg ctagaagacc agaaagaagg 5041 acggagtact aataaggaaa atcctagtaa ggccttgatt gaaaggccca gccaaaataa 5101 cataggaatc caaaccatgg agtgttcctt gagggtccca gaaactgttt cagcagcaac 5161 ccagactata aagaatgtgt gtgagcaggg gaccagtaca gtggaccaga actttggaaa 5221 gcaagatgcc acagttcaga ctgagagggg gagtggtgag aaaccagtca gtgctcctgg 5281 ggatgataca gagtcgctcc atagccaggg agaagaagag tttgatatgc ctcagcctcc 5341 acatggccat gtcttacatc gtcacatgag aacaatccgg gaagtacgca cacttgtcac 5401 tcgtgtcatt acagatgtgt attatgtgga tggaacagaa gtagaaagaa aagtaactga 5461 ggagactgaa gagccaattg tagagtgtca ggagtgtgaa actgaagttt ccccttcaca 5521 gactgggggc tcctcaggtg acctggggga tatcagctcc ttctcctcca aggcatccag 5581 cttacaccgc acatcaagtg ggacaagtct ctcagctatg cacagcagtg gaagctcagg 5641 gaaaggagcc ggaccactca gagggaaaac cagcgggaca gaacccgcag attttgcctt 5701 acccagctcc cgaggaggcc caggaaaact gagtcctaga aaaggggtca gtcagacagg 5761 gacgccagtg tgtgaggagg atggtgatgc aggccttggc atcagacagg gagggaaggc 5821 tccagtcacg cctcgtgggc gtgggcgaag gggccgccca ccttctcgga ccactggaac 5881 cagagaaaca gctgtgcctg gccccttggg catagaggac atttcaccta acttgtcacc 5941 agatgataaa tccttcagcc gtgtcgtgcc ccgagtgcca gactccacca gacgaacaga 6001 tgtgggtgct ggtgctttgc gtcgtagtga ctctccagaa attcctttcc aggctgctgc 6061 tggcccttct gatggcttag atgcctcctc tccaggaaat agctttgtag ggctccgtgt 6121 tgtagccaag tggtcatcca atggctactt ttactctggg aaaatcacac gagatgtcgg 6181 agctgggaag tataaattgc tctttgatga tgggtacgaa tgtgatgtgt tgggcaaaga 6241 cattctgtta tgtgacccca tcccgctgga cactgaagtg acggccctct cggaggatga 6301 gtatttcagt gcaggagtgg tgaaaggaca taggaaggag tctggggaac tgtactacag 6361 cattgaaaaa gaaggccaaa gaaagtggta taagcgaatg gctgtcatcc tgtccttgga 6421 gcaaggaaac agactgagag agcagtatgg gcttggcccc tatgaagcag taacacctct 6481 tacaaaggca gcagatatca gcttagacaa tttggtggaa gggaagcgga aacggcgcag 6541 taacgtcagc tccccagcca cccctactgc ctccagtagc agcagcacaa cccctacccg 6601 aaagatcaca gaaagtcctc gtgcctccat gggagttctc tcaggcaaaa gaaaacttat 6661 cacttctgaa gaggaacggt cccctgccaa gcgaggtcgc aagtctgcca cagtaaaacc 6721 tggtgcagta ggggcaggag agtttgtgag cccctgtgag agtggagaca acaccggtga 6781 accctctgcc ctggaagagc agagagggcc tttgcctctc aacaagacct tgtttctggg 6841 ctacgcattt ctccttacca tggccacaac cagtgacaag ttggccagcc gctccaaact 6901 gccagatggt cctacaggaa gcagtgaaga agaggaggaa tttttggaaa ttcctccttt 6961 caacaagcag tatacagaat cccagcttcg agcaggagct ggctatatcc ttgaagattt 7021 caatgaagcc cagtgtaaca cagcttacca gtgtcttcta attgcggatc agcattgtcg 7081 aacccggaag tacttcctgt gccttgccag tgggattcct tgtgtgtctc atgtctgggt 7141 ccatgatagt tgccatgcca accagctcca gaactaccgt aattatctgt tgccagctgg 7201 gtacagcctt gaggagcaaa gaattctgga ctggcaaccc cgtgaaaatc ctttccagaa 7261 tctgaaggta ctcttggtat cagaccaaca gcagaacttc ctggagctct ggtctgagat 7321 cctcatgact ggtggtgcag cctctgtgaa gcagcaccat tcaagtgccc ataacaaaga 7381 tattgcttta ggggtatttg atgtggtggt gacggacccc tcatgcccag cctcggtgct 7441 gaagtgtgct gaagcattgc agctgcctgt ggtgtcacaa gagtgggtga tccagtgcct 7501 cattgttggg gagagaattg gattcaagca gcatccaaaa tataaacacg attatgtttc 7561 tcactaaagg ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 7621 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 7681 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 7741 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 7801 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 7861 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 7921 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 7981 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 8041 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 8101 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg 8161 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac 8221 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat 8281 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat 8341 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt 8401 ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg 8461 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat 8521 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga 8581 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat 8641 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag 8701 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc 8761 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg 8821 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct 8881 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg 8941 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac 9001 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga 9061 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc 9121 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag 9181 gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 9241 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 9301 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 9361 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 9421 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 9481 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 9541 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 9601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 9661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 9721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 9781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 9841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 9901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 9961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 10021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 10081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 10141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 10201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 10261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 10321 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 10381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 10441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 10501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 10561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 10621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 10681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 10741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 10801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 10861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 10921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 10981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 11041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 11101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 11161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 11221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 11281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 11341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 11401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 11461 gccgcaaaaa agggaataag ggcgacgcgg aaatgttgaa tactcatact cttccttttt 11521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 11581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 11641 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 11701 atgccgcata //