LOCUS       Exported               11232 bp ds-DNA     circular SYN 09-JUN-2021
DEFINITION  Non-integrating (episomal) expression of human L-MYC and LIN28.
ACCESSION   .
VERSION     .
KEYWORDS    pCE-hUL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(83..102)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    148..203
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(202..221)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(564..580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(564..580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(577..599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    588..604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(612..642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    657..678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             1157..3079
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAG?GGGAGGAGGAGAGGGAG?GGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGG?GAGGAGGAGAGG
                     GAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGG
                     SGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPP
                     GRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRR
                     KKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLY
                     NLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKD
                     AIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEG
                     EEGQE"
     rep_origin      3381..5170
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(5711..5728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5882..6470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5962..5981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6641..7501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7264..7283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7502..7606)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7637..8016
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8018..8295
                     /label=chicken beta-actin promoter
     intron          8296..9312
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     9236..9258
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     9320..9339
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             10493..10558
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
       61 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
      121 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
      181 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
      241 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
      301 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
      361 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
      421 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
      481 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
      541 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      721 tcgtgccagc ggatctcaat tctcatgttt gacagcttat catcgataag ctgatcctca
      781 caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa
      841 gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag
      901 gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt
      961 tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca
     1021 gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg
     1081 ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc
     1141 tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg
     1201 agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg
     1261 gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg
     1321 agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa
     1381 acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc
     1441 gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
     1501 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag saggaggagg
     1561 ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagsaggag gaggggcagg
     1621 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc
     1681 aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg
     1741 ggcaggagca ggaggagggg caggagcagg aggggcagga ggggcaggag gggcaggagc
     1801 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggggc
     1861 aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag gggcaggagc
     1921 aggaggggca ggaggggcag gagcaggagg ggcaggaggg gsaggagcag gaggggcagg
     1981 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg
     2041 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggagg
     2101 ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag gcagtggagg
     2161 ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc gccggggtag
     2221 aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag gtcgtggacg
     2281 tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt ctccaccgcg
     2341 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga
     2401 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca
     2461 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg
     2521 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc
     2581 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag
     2641 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc
     2701 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt
     2761 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga
     2821 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa
     2881 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac
     2941 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg
     3001 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg
     3061 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg
     3121 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat
     3181 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgggcatacc
     3241 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg
     3301 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac
     3361 ctaagaatgg gagcaaccag caggaaaagg acaagcagcg aaaattcacg cccccttggg
     3421 aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc atatgctgac
     3481 tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc atatactacc
     3541 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
     3601 cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
     3661 cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc atatgctacc
     3721 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccaga
     3781 tataaattag gatagcatat actaccctaa tctctattag gatagcatat gctacccgga
     3841 tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     3901 tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga
     3961 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     4021 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccatggcaa
     4081 cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac cctgtgcttg
     4141 gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc gcccctatct
     4201 tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt tttgtgggca
     4261 agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac acccttattt
     4321 tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac tccacaattt
     4381 caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg agccccgttt
     4441 aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt ccactctctt
     4501 tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc ctgcctggga
     4561 cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc atagccataa
     4621 attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg atttctattg
     4681 ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag gggtttgtga
     4741 gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa attttattct
     4801 gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact gttcacaact
     4861 cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag attcaggaga
     4921 gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg gttcactacc
     4981 ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt gggagatatc
     5041 gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc ccgttgggta
     5101 acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg ggaagcatat
     5161 gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg ccctcttgag
     5221 ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag cctcccgtag
     5281 tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc ttcagccaag
     5341 agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct gctccaggat
     5401 gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca actacagtca
     5461 gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc ccagttggca
     5521 agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg ggcagagatg
     5581 tcgtagtcag gtttagttcg tccggggcgg ggatcgatcc tctagagtcg acgctagcgg
     5641 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6301 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7621 aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7681 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7741 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7801 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7861 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7921 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     7981 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     8041 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     8101 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccrgg sggggsgggg
     8161 sggggsgrgg ggsggggsgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     8221 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     8281 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     8341 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     8401 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     8461 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     8521 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     8581 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     8641 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     8701 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     8761 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     8821 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     8881 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     8941 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     9001 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     9061 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     9121 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     9181 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     9241 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     9301 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     9361 gaattcgccc ttcaccatgg actacgactc gtaccagcac tatttctacg actatgactg
     9421 cggggaggat ttctaccgct ccacggcgcc cagcgaggac atctggaaga aattcgagct
     9481 ggtgccatcg ccccccacgt cgccgccctg gggcttgggt cccggcgcag gggacccggc
     9541 ccccgggatt ggtcccccgg agccgtggcc cggagggtgc accggagacg aagcggaatc
     9601 ccggggccac tcgaaaggct ggggcaggaa ctacgcctcc atcatacgcc gtgactgcat
     9661 gtggagcggc ttctcggccc gggaacggct ggagagagct gtgagcgacc ggctcgctcc
     9721 tggcgcgccc cgggggaacc cgcccaaggc gtccgccgcc ccggactgca ctcccagcct
     9781 cgaagccggc aacccggcgc ccgccgcccc ctgtccgctg ggcgaaccca agacccaggc
     9841 ctgctccggg tccgagagcc caagcgactc ggagaatgaa gaaattgatg ttgtgacagt
     9901 agagaagagg cagtctctgg gtattcggaa gccggtcacc atcacggtgc gagcagaccc
     9961 cctggatccc tgcatgaagc atttccacat ctccatccat cagcaacagc acaactatgc
    10021 tgcccgtttt cctccagaaa gctgctccca agaagaggct tcagagaggg gtccccaaga
    10081 agaggttctg gagagagatg ctgcagggga aaaggaagat gaggaggatg aagagattgt
    10141 gagtccccca cctgtagaaa gtgaggctgc ccagtcctgc caccccaaac ctgtcagttc
    10201 tgatactgag gatgtgacca agaggaagaa tcacaacttc ctggagcgca agaggcggaa
    10261 tgacctgcgt tcgcgattct tggcgctgag ggaccaggtg cccaccctgg ccagctgctc
    10321 caaggccccc aaagtagtga tcctaagcaa ggccttggaa tacttgcaag ccctggtggg
    10381 ggctgagaag aggatggcta cagagaaaag acagctccga tgccggcagc agcagttgca
    10441 gaaaagaatt gcatacctca ctggctacgg agatctcaaa attgtcgctc ctgtcaaaca
    10501 aactcttaac tttgatttac tcaaactggc tggggatgta gaaagcaatc caggtccact
    10561 catgaacaat tcgcccttca ccatgggctc cgtgtccaac cagcagtttg caggtggctg
    10621 cgccaaggcg gcagaagagg cgcccgagga ggcgccggag gacgcggccc gggcggcgga
    10681 cgagcctcag ctgctgcacg gtgcgggcat ctgtaagtgg ttcaacgtgc gcatggggtt
    10741 cggcttcctg tccatgaccg cccgcgccgg ggtcgcgctc gaccccccag tggatgtctt
    10801 tgtgcaccag agtaagctgc acatggaagg gttccggagc ttgaaggagg gtgaggcagt
    10861 ggagttcacc tttaagaagt cagccaaggg tctggaatcc atccgtgtca ccggacctgg
    10921 tggagtattc tgtattggga gtgagaggcg gccaaaagga aagagcatgc agaagcgcag
    10981 atcaaaagga gacaggtgct acaactgtgg aggtctagat catcatgcca aggaatgcaa
    11041 gctgccaccc cagcccaaga agtgccactt ctgccagagc atcagccata tggtagcctc
    11101 atgtccgctg aaggcccagc agggccctag tgcacaggga aagccaacct actttcgaga
    11161 ggaagaagaa gaaatccaca gccctaccct gctcccggag gcacagaatt gaaagggcga
    11221 attgcctgca gg
//
