LOCUS       Exported               12629 bp ds-DNA     circular SYN 10-JUN-2021
DEFINITION  Eukaryotic expression of human SCN8A variant 1 isoform. This channel
            has been modified to be stably maintained in standard bacterial 
            strains..
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   DeKeyser JM , Thompson CH,  George Jr. AL.
  TITLE     Cryptic prokaryotic promoters explain instability of recombinant 
            neuronal sodium channels in bacteria
  JOURNAL   J Biol. Chem. (2021)
REFERENCE   2  (bases 1 to 12629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5164..5296
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    6984..7568
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7150..7167)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7377..7396
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7569..8264
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(7702..7720)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7970..7989
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8318..8347
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8363..8380
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8403..8420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8409..8633
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8679..9107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8766..8785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8976..8997
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9116..9136)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9121..9450
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9301..9436
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9363..9382
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9498..9545
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9564..9938
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10068..10189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10105..10124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10159..10178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10238..10254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10238..10254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10251..10273)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10262..10278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10286..10316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10331..10352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10469..10486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10640..11228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10720..10739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11399..12259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12022..12041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12260..12364)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12439..12458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc gccatggcag cgcggctgct tgcaccacca ggccctgata gtttcaagcc
      841 tttcacccct gagtcactgg caaacattga gaggcgcatt gctgagagca agctcaagaa
      901 accaccaaag gccgatggca gtcatcggga ggacgatgag gacagcaagc ccaagccaaa
      961 cagcgacctg gaagcaggga agagtttgcc tttcatctac ggggacatcc cccaaggcct
     1021 ggttgcagtt cccctggagg actttgaccc atactatttg acgcagaaaa cctttgtagt
     1081 attaaacaga gggaaaactc tcttcagatt tagtgccacg cctgccttgt acattttaag
     1141 tccttttaac ctgataagaa gaatagctat taaaattttg atacattcag tatttagcat
     1201 gatcattatg tgcactattt tgaccaactg tgtattcatg acttttagta accctcctga
     1261 ctggtcgaag aatgtggagt acacgttcac agggatttat acatttgaat cactagtgaa
     1321 aatcattgca agaggtttct gcatagatgg ctttaccttt ttacgggacc catggaactg
     1381 gttagatttc agtgtcatca tgatggcgta tataacagag tttgtaaacc taggcaatgt
     1441 ttcagctcta cgcactttca gggtactgag ggctttgaaa actatttcgg taatcccagg
     1501 cctgaagaca attgtgggtg ccctgattca gtctgtgaag aaactgtcag atgtgatgat
     1561 cctgacagtg ttctgcctga gtgtttttgc cttgatcgga ctgcagctgt tcatggggaa
     1621 ccttcgaaac aagtgtgttg tgtggcccat aaacttcaac gagagctatc ttgaaaatgg
     1681 caccaaaggc tttgattggg aagagtatat caacaataaa acaaatttct acacagttcc
     1741 tggcatgctg gaacctttac tctgtgggaa cagttctgat gctgggcaat gcccagaggg
     1801 ataccagtgt atgaaagcag gaaggaaccc caactatggt tacacaagtt ttgacacttt
     1861 tagctgggcc ttcttggcat tatttcgcct tatgacccag gactattggg aaaacttgta
     1921 tcaattgact ttacgagcag ccgggaaaac atacatgatc ttcttcgtct tggtcatctt
     1981 tgtgggttct ttctatctgg tgaacttgat cttggctgtg gtggccatgg cttatgaaga
     2041 acagaatcag gcaacactgg aggaggcaga acaaaaagag gctgaattta aagcaatgtt
     2101 ggagcaactt aagaagcaac aggaagaggc acaggctgct gcgatggcca cttcagcagg
     2161 aactgtctca gaagatgcca tagaggaaga aggtgaagaa ggagggggct cccctcggag
     2221 ctcttctgaa atctctaaac tcagctcaaa gagtgcaaag gaaagacgta acaggagaaa
     2281 gaagaggaag caaaaggaac tctctgaagg agaggagaaa ggggatcccg agaaggtgtt
     2341 taagtcagag tcagaagatg gcatgagaag gaaggccttt cggctgccag acaacagaat
     2401 agggaggaaa ttttccatca tgaatcagtc actgctcagc atcccaggct cgcccttcct
     2461 ctcccgccac aacagcaaga gcagcatctt cagtttcagg ggacctgggc ggttccgaga
     2521 cccgggctcc gagaatgagt tcgcggatga cgagcacagc acggtggagg agagcgaggg
     2581 ccgccgggac tccctcttca tccccatccg ggcccgcgag cgccggagca gctacagcgg
     2641 ctacagcggc tacagccagg gcagccgctc ctcgcgcatc ttccccagcc tgcggcgcag
     2701 cgtgaagcgc aacagcacgg tggactgcaa cggcgtggtg tccctcatcg gcggccccgg
     2761 ctcccacatc ggcgggcgtc tcctgccaga ggctacaact gaggtggaaa ttaagaagaa
     2821 aggccctgga tctcttttag tttccatgga ccaattagcc tcctacgggc ggaaggacag
     2881 aatcaacagt ataatgagtg ttgttacaaa tacactagta gaagaactgg aagagtctca
     2941 gagaaagtgc ccgccatgct ggtataaatt tgccaacact ttcctcatct gggagtgcca
     3001 cccctactgg ataaaactga aagagattgt gaacttgata gttatggacc cttttgtgga
     3061 tttagccatc accatctgca tcgtcctgaa tacactgttt atggcaatgg agcaccatcc
     3121 tatgacacca caatttgaac atgtcttggc tgtaggaaat ctggtaagta tgaaattcag
     3181 ggatacggca tatttgccaa atagtggaaa tgtgaagtac tgacaaaact tttccctttt
     3241 tccctctcat aggttttcac tggaattttc acagcggaaa tgttcctgaa gctcatagcc
     3301 atggatccct actattattt ccaagaaggt tggaacattt ttgacggatt tattgtctcc
     3361 ctcagtttaa tggaactgag tctagcagac gtggaggggc tttcagtgct gcgatctttc
     3421 cgattgctcc gagtcttcaa attggccaaa tcctggccca ccctgaacat gctaatcaag
     3481 attattggaa attcagtggg tgccctgggc aacctgacac tggtgctggc cattattgtc
     3541 ttcatctttg ccgtggtggg gatgcaactc tttggaaaaa gctacaaaga gtgtgtctgc
     3601 aagatcaacc aggactgtga actccctcgc tggcatatgc atgacttttt ccattccttc
     3661 ctcattgtct ttcgagtgtt gtgcggggag tggattgaga ccatgtggga ctgcatggaa
     3721 gtggcaggcc aggccatgtg cctcattgtc tttatgatgg tcatggtgat tggcaacttg
     3781 gtggtgctga acctgtttct ggccttgctc ctgagctcct tcagtgcaga caacctggct
     3841 gccacagatg acgatgggga aatgaacaac ctccagatct cagtgatccg tatcaagaag
     3901 ggtgtggcct ggaccaaact aaaggtgcac gccttcatgc aggcccactt taagcagcgt
     3961 gaggctgatg aggtgaagcc tctggatgag ttgtatgaaa agaaggccaa ctgtatcgcc
     4021 aatcacaccg gtgcagacat ccaccggaat ggtgacttcc agaagaatgg caatggcaca
     4081 accagcggca ttggcagcag cgtggagaag tacatcattg atgaggacca catgtccttc
     4141 atcaacaacc ccaacttgac tgtacgggta cccattgctg tgggcgagtc tgactttgag
     4201 aacctcaaca cagaggatgt tagcagcgag tcggatcctg aaggcagcaa agataaacta
     4261 gatgacacca gctcctctga aggaagcacc attgatatca aaccagaagt agaagaggtc
     4321 cctgtggaac agcctgagga atacttggat ccagatgcct gcttcacaga aggttgtgtc
     4381 cagcggttca agtgctgcca ggtcaacatc gaggaagggc taggcaagtc ttggtggatc
     4441 ctgcggaaaa cctgcttcct catcgtggag cacaactggt ttgagacctt catcatcttc
     4501 atgattctgc tgagcagtgg cgccctggcc ttcgaggaca tctacattga gcagagaaag
     4561 accatccgca ccatcctgga atatgctgac aaagtcttca cctatatctt catcctggag
     4621 atgttgctca agtggacagc ctatggcttc gtcaagttct tcaccaatgc ctggtgttgg
     4681 ctggacttcc tcattgtggc tgtctcttta gtcagcctta tagctaatgc cctgggctac
     4741 tcggaactag gtgccataaa gtcccttagg accctaagag ctttgagacc cttaagagcc
     4801 ttatcacgat ttgaagggat gagggtggtg gtgaatgcct tggtgggcgc catcccctcc
     4861 atcatgaatg tgctgctggt gtgtctcatc ttctggctga ttttcagcat catgggagtt
     4921 aacttgtttg cgggaaagta ccactactgc tttaatgaga cttctgaaat ccgatttgaa
     4981 attgaagatg tcaacaataa gaccgaatgt gaaaagctta tggaggggaa caatacagag
     5041 atcagatgga agaacgtgaa gatcaacttt gacaatgttg gggcaggata cctggccctt
     5101 cttcaagtag caaccttcaa aggctggatg gacatcatgt atgcagctgt agattcccgg
     5161 aaggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     5221 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     5281 gcctttctct ccacagcctg atgagcagcc taagtatgag gacaatatct acatgtacat
     5341 ctattttgtc atcttcatca tcttcggctc cttcttcacc ctgaacctgt tcattggtgt
     5401 catcattgat aacttcaatc aacaaaagaa aaagttcgga ggtcaggaca tcttcatgac
     5461 cgaagaacag aagaagtact acaatgccat gaaaaagctg ggctcaaaga agccacagaa
     5521 acctattccc cgccccttga acaaaatcca aggaatcgtc tttgattttg tcactcagca
     5581 agcctttgac attgttatca tgatgctcat ctgccttaac atggtgacaa tgatggtgga
     5641 gacagacact caaagcaagc agatggagaa catcctctac tggattaacc tggtgtttgt
     5701 tatcttcttc acctgtgagt gtgtgctcaa aatgtttgcg ttgaggcact actacttcac
     5761 cattggctgg aacatcttcg acttcgtggt agtcatcctc tccattgtgg gaatgttcct
     5821 ggcagatata attgagaaat actttgtttc cccaacccta ttccgagtca tccgattggc
     5881 ccgtattggg cgcatcttgc gtctgatcaa aggcgccaaa gggattcgta ccctgctctt
     5941 tgccttaatg atgtccttgc ctgccctgtt caacatcggc cttctgctct tcctggtcat
     6001 gttcatcttc tccatttttg ggatgtccaa ttttgcatat gtgaagcacg aggctggtat
     6061 cgatgacatg ttcaactttg agacatttgg caacagcatg atctgcctgt ttcaaatcac
     6121 aacctcagct ggttgggatg gcctgctgct gcccatccta aaccgccccc ctgactgcag
     6181 cctagataag gaacacccag ggagtggctt taagggagat tgtgggaacc cctcagtggg
     6241 catcttcttc tttgtaagct acatcatcat ctctttccta attgtcgtga acatgtacat
     6301 tgccatcatc ctggagaact tcagtgtagc cacagaggaa agtgcagacc ctctgagtga
     6361 ggatgacttt gagaccttct atgagatctg ggagaagttc gaccccgatg ccacccagtt
     6421 cattgagtac tgtaagctgg cagactttgc agatgccttg gagcatcctc tccgagtgcc
     6481 caagcccaat accattgagc tcatcgctat ggatctgcca atggtgagcg gggatcgcat
     6541 ccactgcttg gacatccttt ttgccttcac caagcgggtc ctgggagata gcggggagtt
     6601 ggacatcctg cggcagcaga tggaagagcg gttcgtggca tccaatcctt ccaaagtgtc
     6661 ttacgagcca atcacaacca cactgcgtcg caagcaggag gaggtatctg cagtggtcct
     6721 gcagcgtgcc taccggggac atttggcaag gcggggcttc atctgcaaaa agacaacttc
     6781 taataagctg gagaatggag gcacacaccg ggagaaaaaa gagagcaccc catctacagc
     6841 ctccctcccg tcctatgaca gtgtaactaa acctgaaaag gagaaacagc agcgggcaga
     6901 ggaaggaaga agggaaagag ccaaaagaca aaaagaggtc agagaatcca agtgttgact
     6961 cgagcgatcg gcccttggat ccgcccctct ccctcccccc ccctaacgtt actggccgaa
     7021 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     7081 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     7141 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     7201 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     7261 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     7321 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     7381 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     7441 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
     7501 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     7561 ccacaaccat ggtgagcaag ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc
     7621 acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     7681 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct
     7741 cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcacc aagcaccccg
     7801 ccgacatccc cgactactat aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga
     7861 tgaacttcga ggacggcggc gccgtgaccg tgacccagga cacctccctg gaggacggca
     7921 ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc
     7981 agaagaagac aatgggctgg gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc
     8041 tgaagggcga cattaagatg gccctgcgcc tgaaggacgg aggccgctac ctggcggact
     8101 tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc
     8161 gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct
     8221 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaacct gcaggaattc
     8281 aagggcgaat tcgtttgctc tagagggccc gcggttcgaa caaaaactca tctcagaaga
     8341 ggatctgaat atgcataccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     8401 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     8461 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     8521 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     8581 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     8641 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     8701 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8761 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8821 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8881 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8941 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9001 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     9061 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     9121 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9181 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9241 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     9301 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9361 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     9421 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     9481 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     9541 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     9601 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     9661 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     9721 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     9781 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     9841 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     9901 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     9961 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    10021 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    10081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    10141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    10201 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    10261 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10321 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10381 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10441 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10501 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10561 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10621 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10681 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10741 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10801 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10861 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10921 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10981 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11041 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    11101 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11161 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11221 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11281 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11341 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11401 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11461 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11521 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11581 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11641 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11701 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11761 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11821 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11881 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11941 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12001 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    12061 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    12121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    12181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    12241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    12301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12361 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    12421 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    12481 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    12541 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    12601 tcgcgatgta cgggccagat atacgcgtt
//
