LOCUS       Exported               13901 bp ds-DNA     circular SYN 11-JUN-2021
DEFINITION  Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish 
            larva. The plasmid is in Tol2 transposon backbone.
ACCESSION   .
VERSION     .
KEYWORDS    Tol2-elavl3-GCaMP6s
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   Vladimirov N, Mu Y, Kawashima T, Bennett DV, Yang CT, Looger LL, 
            Keller PJ, Freeman J, Ahrens MB
  TITLE     Light-sheet functional imaging in fictively behaving zebrafish.
  JOURNAL   Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3040.
  PUBMED    25068735
REFERENCE   2  (bases 1 to 13901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13901
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             9463..10815
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      9559..9568
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     9849..9870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9976..9997)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10237..10256)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    10858..10992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10882..10901)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10936..10955
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(11664..11680)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11664..11680)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11717..11737)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11717..11735)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11756..11772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11756..11772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11769..11791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11780..11796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11804..11834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11849..11870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11987..12004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12158..12746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12238..12257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12917..13777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13540..13559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13778..13882)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttcccg
      721 ggtgtcgacg aattctcgag ctctattcct aaagaccttg ggtgaccaaa atcttatttt
      781 aataaataaa actgtttatt aaaacttttt tgtttcaaag aaccatatgt atagtgaaat
      841 ttataaaaat atcaattttt aaaaagctgg tgtactcatt tatgttatga actctaaaac
      901 catatactga ctgcaagtga tgatgtatag agtgatgttt acgagtaaac atatttagtt
      961 gtatacatcc tactgagcac attttgatgt atgaaataac attacaagct ttatccaaat
     1021 taagccattt taaaacactg ccaattgaaa atacaaatcc tggaaaaaat cgtctttagc
     1081 gcagtcattt gagccatcct aatccgttac ctcagaccat aataagaagg gataacacta
     1141 gctgtagcaa tggaacacat ctgtttcaca caatcatatc tcctgcgccg gtgctaagca
     1201 gattcagcgt gatcataaca tgctttccac tcataaatgt aaatttacaa tttgcacatg
     1261 taaaacagac acttttgaga tattggataa aaaaacaaga gtatattgct tagtttcatc
     1321 caccagtcat ccccacagcg tttggaaggc cataaaaagt gtctaaaatc aatgatcatt
     1381 gaaagagcac aagagagact cttacgctgt aatgccactg gggacaaaag tgacagtctc
     1441 ttaatgggct cttctggagg ggctcctgaa cattaaaaat tatcagcgaa attaccgaaa
     1501 gagcttcaag caactggcat gcttgatcct ctgcgtcggg gcggtgaata ggtgcttcag
     1561 atgccctctt acccacgggc tggattcagc tgccccgcta ccagcggaga ccccctaatg
     1621 agcctctgca attaagttta ttcatgttaa gtgtgaacgg ggtgcgtgcg gaactgtggg
     1681 cagctaacag acctgggttc tttgtgccac aagtgctgcc tttattcggc tcacaaagca
     1741 gaaaacaaca cccgcaccta taatggcgcc ctcggctggg tctaagaaac gtggcgagtt
     1801 gacagagcag agtgggcggg gttaagacag actgacagcg ggacccatct ccatcctctt
     1861 attaacgctt aacgagtgcc ttcctcatgc aatattcatc gccactaata tcatccaagc
     1921 tctgagctga gctggccact tatgtaaggc aattatgtaa aatatcagac agggcccaca
     1981 ctcagaatct gactggggta gagacgcggg acgagaaccg agagcaagaa ctgaaagtga
     2041 aagtgaccac taaagggagg agaggacaga ggggcaggat gtgtcaagat taccagagaa
     2101 cacttggcca gaaatgcgca accattggag ctctccggat tacccaaagg ttaacgagtt
     2161 tgaacgcctc tgcccactcg cccatctctg atggtttccc aagaactcct caagcaaaat
     2221 atatataatt gtgtgtatta tgcacagaca cgagaaaatg ctgtttttct gatctgcatt
     2281 acagcacatt tgcccgccaa cgacaatacc acccactcgg tacctcgctg actcctgatg
     2341 cctgatacct gcgcggtgac tgtctacaat ctgcataatc aagagaagtt gtgttgaaga
     2401 cgagcgccac acaaccgttt ccacaaggtc acccaaggcc ggtgcagatg taggtgaggt
     2461 ctccataaac agactgaaat aaacacatcc tccgctggga acaacaaccc cctcacgcct
     2521 catgcatttc cataagccta catgcatctc ttccaactta tggagactcg cacctaccaa
     2581 catccgcaca acaaagatat acagagcgcg ctccctcagg tcaaggccct gtgggggtct
     2641 gtgcagaaat aggtcatttg tcacacatca agtcctgggg caggagatgc attatagatg
     2701 agaccaaaca gcctgtctcg gtgagctcta cccactccct gagactagaa atgggggaag
     2761 ggagcttgag ataacaaccg ctgcaatcac tgtgtcgatg tttaatatca gcaccaaccg
     2821 ggaacaataa ggagatgggt gcattcatgt tcacatctta ccagtcaagt atcatcgaac
     2881 cggcttgata accacacctc gtgtaatagc tgagcagata gttgtcattt taaagcgttg
     2941 gcctttgtcg attatgtaat gcgcacattc aacacatggt aatatagaaa cggttatgtc
     3001 gaggttgttt tgtccagaga tgaccttcac acagttacag ccgctctgca tccacacaaa
     3061 tggaggactt aatcgtggac tgcattctta gaaatgatct acaaagacaa ataatgtgaa
     3121 atcaagaaag gacaaaattt aagtaagggg atgagggaga gagagaacga ggggcaagga
     3181 gaaagcatgg ctcctgtctt tttctgcacc catctgttcg gagtgcaggt ggagctctat
     3241 tcactcagct ctgcatgtgt gtttgggggg ggcaggaaga aagggagggc aaaaggaaga
     3301 gtggagagat ggtgggggct ggagggatgg ggggttctcg gtgatctctc ctgaagggga
     3361 taatgggaga gcagcgcttt gcaatggctg ccatgtagta ccctccctgc acaattagcc
     3421 aatcagcagc aagctctgcc agccagaagg acacataaaa gaagaacatt gcagcagagg
     3481 cacagaagga gcctgcgagg agctgggaaa tacacacaca acagcagaac cacaacaccc
     3541 tcccctggac acaccctact ggggatcact gcttttcttt ttttctgaac catcgcccac
     3601 gccacacgga gagaaatctc tctctcatca tcatcctgaa gaaaaccccc ttatcctcat
     3661 tttcacactg ctgaggaaaa cctacaatcg cacgggctga gatttcctgg cgaagactgt
     3721 cctttttcct ttttcttttt ttttcttttc ctttggaaac tgacatttgc atttctccat
     3781 tccaagccac ggcgtaataa tatctgcaat ccagcctgaa gacctgcaaa tcgaaggacc
     3841 tagatcacta tcatctttgt acgtcaagga tcctggttac tgtacgtata acctttcttt
     3901 cttttgctct gaccaatatg aaacactaaa atcgattcga gcagcctctc agcatcaatt
     3961 acagtgcgtg aaaaacattc aaatagaggc agcaaatatt acatgtgaaa atacaggctg
     4021 gctaaatcca agctaattta gaaatgtggt caaaacgcat actggcacgt ctaatcgcgg
     4081 attcagtaaa caagattaac gattagccca gtgtataagt catatgatac cggcatgcgc
     4141 gagagcatcg ctacacccga gctggcttca ttttcggagg aaaatcaaaa cattgctttc
     4201 tcctgccgtg cgaaccattc gtcataaacc gtaatacgca acatacattt attactacat
     4261 ccgttaatta gcgataatta gccgttatta acaaagagcg ctgaggaatt cctctcaaat
     4321 agcggaggtg cggcggaggc agaggggcgt aaaagggcac atgcgtgcct ggctcaaaaa
     4381 aggatgctcc agactgaaac tcagagacaa aacacgatgc ctcggaggct gagagcccat
     4441 cgagaggaaa ggcaaagaaa agggtcgagt cactcagagg gggaggggag atattgtgca
     4501 tccattggtt tgaattgaag caggcagaat aaaaatcctt gcgattatgc ttcgtgagag
     4561 atcgcgagag aggaaaaggc taaatgccac gtatgcaaat ggataaatgt catctatttc
     4621 ttcgcgagta gacattttgt ggacaacgag agtgtaaata agcgatatga ggggaataca
     4681 aaggccttga gggaaagcgt tttgggcgtg tcttgtcgaa aagaagacag cgatggcacg
     4741 cgctcagaac caccatcttc acgtagacaa tccgcgtgaa aacaataaca aaactgctca
     4801 ttttaacacg agcaggtgtt tgaaaccacc caaaaaagat tgtctaaaat attaagatta
     4861 attaatgacg tttaaatgtc caggcctgat ataaatgaag cgtgtagtaa gagtaatcgc
     4921 ctttgtttct tttcagagat acgttttcag agattcaggg aattatatct tcaataaaat
     4981 aatgtttgat ttcattattt taaagctttt taagaagtaa aaatacacat tattcattta
     5041 cattttattt tatttaaagg cctatggtct tgaaaataat aagtggaata tataggctat
     5101 atattcgtct aaggacagcc ggtgttattt tatagtgacc tcagacaaaa gccgagacaa
     5161 aacgaggcgc ttgcctttca ttttaatcct taatagacgg tgtgatgaat gaatgaatga
     5221 atgaataaat gaatgaatga cgggcccttc ggcatgttgt tccgcagtcc tccgtgagca
     5281 cgagtctcta ccgcagtgcc aagcacataa aaccgttgag tcagataacc ttctccaggc
     5341 gcgtatacac aattcaagga caagcacatc acaactatac acaacacaac acaaccgcct
     5401 aaacgcacga gctcatcaat cgcaatttaa aggcatgtca gtcaaagaaa aaggcggcgt
     5461 tatttaaaac caatgttcaa caatatttgt atgctgacta gcccgggcgc gcacgctata
     5521 agatcacaag gccttgtttt ttccctttat tcaaaatatc attaattatt acaggtcata
     5581 aaacaaaaca atgcgaatta caatgattta aagactattg atttaacaat aatcagttaa
     5641 ttatagaata atattataat attttacata caaatgaaag atgacattat ttgatgacat
     5701 tgtaattaaa atatatgcaa tatattttta cgtttttatt aattaggcta ttactattat
     5761 tataattatc attattttta ttattattat tattattatt aaatagtctt gcgaaataag
     5821 agtgaaggca gttgcaatgt tcattgttcc cggagagtcc cgcagcctct ggcctcaaca
     5881 caaagcgcta ctgtttacat taatattcat agtgctgcca tggtctctgt agaggaggaa
     5941 cgggtggctc ttttgttcgc tagtgtcaat acgtttattc agcaaaaagc agctcggatt
     6001 tgtgaatgcc caacaagcac acgcgcactc caatcaaagg acgagaggat aacgatttta
     6061 aacgattcgc aaacgtgttt atttgtaaag acgacatcaa gagcttaatg tccacttgaa
     6121 aagaaataaa tatgcccgcg gcgccaagga tattttgaat gtagtcgttc atggtgagcc
     6181 actcccgaat tcagcactct ggagagcgac caaagaggaa tagcggaata gcaactactt
     6241 gagccatttc tctccgtttg atggcgttca tgattaaaaa tataatcacg tgatttttta
     6301 tacatatata aatatataca taaagtaacg ctttcctttt gagaatacat aaatatttat
     6361 tcgcgtgata aatcaatgat cttatattta tttgcacgac cgaaattagc caaattcacc
     6421 aattaaaaaa aaaataagcc aacaaaaaag aagggtttat ccgttcaatt ttgacttgtg
     6481 tcctgttgtt ttacagctgc cactgtgatc ccttaagctg cagtgagagt gtggctaatg
     6541 ccttttgttt aagataatcc tgtaatctgt taccgaaacg gcctattgac aagccggcat
     6601 tcacatttca gtcaggaggc ccgtccagac atgtacattt atgaattacg aatgataaaa
     6661 ttaagatctg cattaggcgt cagaaatgtc acgaacaccc attcatttcc cacagccaat
     6721 aatggatcat gctgggaagc gctatcctcg cagtctcaaa attaatgttg acatgttgcg
     6781 tagagctaca gattagatca gccatatgct ttatgtgttt acctcagcgg atcttcagca
     6841 agatgtgttt attttaagaa aatggcttcc tcgctgcctg cacaggggca ttaaggaagt
     6901 gacgtgagcg tcctcacagt caaattactt tatctcatgc tgctttcagg cctctgctta
     6961 ttttattatt attattttaa attaggggga atcacggtgg cgttgtgggt agggtagcgc
     7021 gatcacctta cagcaagaag gtcgctggtt cgagctctgg ctgggtcagt tggcatttct
     7081 gtgtggagtt taaattgaat tgaaattgaa taaactaaat tggccctagt gtatgtgtgt
     7141 gaatgtaagt gtgcatgggt gtttcccagt gttgggtttg actggaagag catccgcttc
     7201 gtaaaacata tgctggataa gttggcggtt catttcactg tggtgaccgc tgattaataa
     7261 agggactaag ctgaaaatga aatgaattaa tacattttaa atgagctttt gctgacatgt
     7321 ttatgattac aaatagtgaa attgtatgtt aatttacgat aagtgcattg ttctaaattc
     7381 atttttatgt agcagctggt ttttcttcca tttgtgtcat tcaacagaac atttttctaa
     7441 aaaagtgaac ttaaattttc tcctccctct gacaaggctt gatggtaatt tcagccgact
     7501 ctaattacag actcactaaa aggaatcggt gcagtcattt atctttatca gcgcgctggt
     7561 gggaaggtaa taggttttgc ttatgagttg tttgcgtgcg agtctgggtc cttgtgtagg
     7621 cgatgctgat gcactgcatt aaatattgag ggagaagtcg ctcaaaaata gcaaagtaca
     7681 ggctggggca ggaacgtcaa agcccgctca gtggccacgt ttcttgtttc gtaagtccta
     7741 tttttaatag cgataattca ggctcagtag aaggaactcc agcactataa gcagtctctc
     7801 gcctgtctcg gcgagttatg tattttggga tgaagtgctc ttccacctct acctctgctg
     7861 tgctggcgcc agtatctggg ctgtaagata gtgtacgtgt cagtctgtgt ggccgaagag
     7921 cctgtgtgtt gtgtaactca ctacattcgt cgctgtggat ggctgacttg ccatggggag
     7981 gtttacttca gttcacgaat cacattcgcc acgatcttgt cactttgtgt caagactcgc
     8041 tctctgtgtg tcaacagctc tgctgtttga ggttgaaatt ctgaacttga gatatatccc
     8101 aggatatatt agggctactg ccaacggaaa aagcaaatgg tgccatggcc atttgattga
     8161 tcaaacaaac acccttttgc ttgggtgaca gtgaaatgtc agatccatgt ttgtactgct
     8221 ttaataactt gcctctctga gctttctcct tgtcagtgtc aatttgcctg agatataaat
     8281 tccggttgcc ccgaaagctt attttattaa aaaaaagcta acatgcactc atacaacgtg
     8341 cgcacataca tgtgaggctt gctaaaagtc aagaagcaag ccaatagcaa gagtcgtcag
     8401 actgctgtta cctgggcaac ctggttagag gtaaggctca aggcaagaat aaatcattcc
     8461 actgaagaga cacagcgtta cagcgagatg agagcaagag gcgatagtga agggagaaca
     8521 aaagaacagc ctacccacac tagtgcagat ctacctgttt gagctactga ttgttcagtt
     8581 tgattagata tcagacaatt ctgctatctc caattgcatt gctgcatgaa ttcctagaaa
     8641 tcattttaat caccaaaaac tagtagggac acttgtggcg tatatgattc tgcctgttga
     8701 ttgtggggat ccaacagaaa gatcatgaat gcattgttaa ttaagtcggt gagacaccct
     8761 gttccaccct aggggccgtt ctggggaaag agtgctttca gtcagctaaa gatgactaat
     8821 atgtgaacat atattactac acatgccaat ttgtacattc tggatagtta ccagccctgg
     8881 gaaaacatcg aaaaaaataa atcaacatag ataaaattgg caaatccctt ctggtacgat
     8941 gcagagtgct tgctctgaga acttgtgcat tgtgacagaa agcgaccgtt aaaaactgaa
     9001 agtagcatct acaacctgca tacatctgtt ctttgaaaat cccctgtagg ccaaattaag
     9061 catcacgctg tgctccacag caacacggat gaaccgaaaa cccagacaca aacgcacaca
     9121 cttttcacat aaacaaggtt tagttatgct agctatgctt tttcgtcctc cctttgtcat
     9181 cagcggcgag tgacgtaaca cagtttgacc ctggacagcg taaagggggg gttgggaatg
     9241 agtgaaagga ctgtgacatc tgcagggcca ggggctgatg gagagagctg aagtgaggga
     9301 ggtcagagga ggaggaaggg tgggatattg tcctcctcag gccctatccc tctcagacgc
     9361 attgattttt aacctctcag cgagaatgcc aagcattaca cccctcaaat tatggttcct
     9421 cacccaacct gttatatttt ccacctgcag ataattaccg gtatgggttc tcatcatcat
     9481 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
     9541 gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca
     9601 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc
     9661 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacat cgaggacggc
     9721 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     9781 ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag
     9841 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     9901 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
     9961 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
    10021 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
    10081 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
    10141 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
    10201 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
    10261 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
    10321 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg
    10381 actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat
    10441 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
    10501 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
    10561 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa
    10621 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag
    10681 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
    10741 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
    10801 atgacagcga agtgaaccgg tggatcctgg ccgtcgtttt actgtaccat cgatgatgat
    10861 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
    10921 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
    10981 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
    11041 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
    11101 ctagatcaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
    11161 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
    11221 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
    11281 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac
    11341 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt
    11401 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg
    11461 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag
    11521 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa
    11581 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc
    11641 ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga
    11701 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
    11761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    11821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    11881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    11941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    12001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    12061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    12121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    12181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    12241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    12301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    12361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    12421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    12481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    12541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    12601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    12661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    12721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    12781 ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca aaaaggatct
    12841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    12901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    12961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    13021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    13081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    13141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    13201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    13261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    13321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    13381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    13441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    13501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    13561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    13621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    13681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    13741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    13801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    13861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt g
//
