LOCUS       Exported                8606 bp ds-DNA     circular SYN 16-JUN-2021
DEFINITION  Control lentiviral vector expressing Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti.PGK.blast-Renilla_Luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    40..61
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        76..106
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    114..130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     119..141
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     138..154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     138..154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     173..193
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        175..193
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        221..447
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             448..628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    675..800
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1293..1526
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1711..1755
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2022..2139
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2185..2695
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2614..2632
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    2750..2774
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2780..3715
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(3718..3742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3773..4361
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3826..3846)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4244..4255)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4412..4432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4417..4746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4597..4732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4794..4841
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4860..5258
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5345..5578
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5650..5771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5687..5706)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5741..5760
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5811..5946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5966..5985)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5967..5985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5995..6012)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5995..6011)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6004..6026)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6153..6608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6240..6259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6450..6471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6634..6738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6739..7599
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6957..6976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7770..8358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8259..8278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8512..8529
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
       61 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      121 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      181 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
      241 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
      301 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
      361 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
      421 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
      481 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      541 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      601 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      661 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
      721 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
      781 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      841 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
      901 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
      961 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1021 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1081 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1141 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1201 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1261 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1321 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1381 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1441 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1501 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1561 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1621 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1681 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     1741 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     1801 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     1861 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     1921 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     1981 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     2041 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2101 aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga gactagcctc
     2161 gagaagcttg atatcgaatt ccacggggtt ggggttgcgc cttttccaag gcagccctgg
     2221 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
     2281 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
     2341 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
     2401 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
     2461 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
     2521 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
     2581 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
     2641 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga
     2701 tccaccggtt cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
     2761 caaaaaagca ggcttcacca tgacttcgaa agtttatgat ccagaacaaa ggaaacggat
     2821 gataactggt ccgcagtggt gggccagatg taaacaaatg aatgttcttg attcatttat
     2881 taattattat gattcagaaa aacatgcaga aaatgctgtt atttttttac atggtaacgc
     2941 ggcctcttct tatttatggc gacatgttgt gccacatatt gagccagtag cgcggtgtat
     3001 tataccagac cttattggta tgggcaaatc aggcaaatct ggtaatggtt cttataggtt
     3061 acttgatcat tacaaatatc ttactgcatg gtttgaactt cttaatttac caaagaagat
     3121 catttttgtc ggccatgatt ggggtgcttg tttggcattt cattatagct atgagcatca
     3181 agataagatc aaagcaatag ttcacgctga aagtgtagta gatgtgattg aatcatggga
     3241 tgaatggcct gatattgaag aagatattgc gttgatcaaa tctgaagaag gagaaaaaat
     3301 ggttttggag aataacttct tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt
     3361 agaaccagaa gaatttgcag catatcttga accattcaaa gagaaaggtg aagttcgtcg
     3421 tccaacatta tcatggcctc gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt
     3481 acaaattgtt aggaattata atgcttatct acgtgcaagt gatgatttac caaaaatgtt
     3541 tattgaatcg gacccaggat tcttttccaa tgctattgtt gaaggtgcca agaagtttcc
     3601 taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg cacctgatga
     3661 aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac aataaatacc
     3721 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc
     3781 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3841 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3901 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3961 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4021 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4081 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4141 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4201 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4261 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4321 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc
     4381 cggttagtaa tgagtttgga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4441 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4501 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4561 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4621 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4681 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4741 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     4801 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     4861 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     4921 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     4981 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     5041 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     5101 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     5161 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     5221 gtgaattgct gccctctggt tatgtgtggg agggctaagc acaattcgag ctcggtacct
     5281 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5341 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5401 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5461 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5521 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5581 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5641 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5701 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5761 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     5821 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5881 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5941 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6001 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6061 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6121 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6181 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6241 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6301 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6361 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6421 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6481 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6541 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6601 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6781 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6841 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6901 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6961 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7021 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7081 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7141 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7201 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7261 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7321 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7381 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7441 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7501 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7561 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7621 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8461 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8521 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8581 attcattaat gcagctggca cgacag
//
