LOCUS       Exported               12531 bp ds-DNA     circular SYN 16-JUN-2021
DEFINITION  Lentiviral expression of hTERT, used to create immortalized cell 
            lines.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-hTERT-IRES-hygro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 12531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3387..6785
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(7043..7060)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7270..7289
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7451..8476
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    8500..9088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8553..8573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8971..8982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9295..9928
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10056..10072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10056..10072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10069..10091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10080..10096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10104..10134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10149..10170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10287..10304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10458..11046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10538..10557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11217..12077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11840..11859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12078..12182)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12230..12364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12267..12286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12321..12340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccatgc cgcgcgctcc ccgctgccga gccgtgcgct
     3421 ccctgctgcg cagccactac cgcgaggtgc tgccgctggc cacgttcgtg cggcgcctgg
     3481 ggccccaggg ctggcggctg gtgcagcgcg gggacccggc ggctttccgc gcgctggtgg
     3541 cccagtgcct ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc ccctccttcc
     3601 gccaggtgtc ctgcctgaag gagctggtgg cccgagtgct gcagaggctg tgcgagcgcg
     3661 gcgcgaagaa cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc gggggccccc
     3721 ccgaggcctt caccaccagc gtgcgcagct acctgcccaa cacggtgacc gacgcactgc
     3781 gggggagcgg ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg ctggttcacc
     3841 tgctggcacg ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac caggtgtgcg
     3901 ggccgccgct gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca cacgctagtg
     3961 gaccccgaag gcgtctggga tgcgaacggg cctggaacca tagcgtcagg gaggccgggg
     4021 tccccctggg cctgccagcc ccgggtgcga ggaggcgcgg gggcagtgcc agccgaagtc
     4081 tgccgttgcc caagaggccc aggcgtggcg ctgcccctga gccggagcgg acgcccgttg
     4141 ggcaggggtc ctgggcccac ccgggcagga cgcgtggacc gagtgaccgt ggtttctgtg
     4201 tggtgtcacc tgccagaccc gccgaagaag ccacctcttt ggagggtgcg ctctctggca
     4261 cgcgccactc ccacccatcc gtgggccgcc agcaccacgc gggcccccca tccacatcgc
     4321 ggccaccacg tccctgggac acgccttgtc ccccggtgta cgccgagacc aagcacttcc
     4381 tctactcctc aggcgacaag gagcagctgc ggccctcctt cctactcagc tctctgaggc
     4441 ccagcctgac tggcgctcgg aggctcgtgg agaccatctt tctgggttcc aggccctgga
     4501 tgccagggac tccccgcagg ttgccccgcc tgccccagcg ctactggcaa atgcggcccc
     4561 tgtttctgga gctgcttggg aaccacgcgc agtgccccta cggggtgctc ctcaagacgc
     4621 actgcccgct gcgagctgcg gtcaccccag cagccggtgt ctgtgcccgg gagaagcccc
     4681 agggctctgt ggcggccccc gaggaggagg acacagaccc ccgtcgcctg gtgcagctgc
     4741 tccgccagca cagcagcccc tggcaggtgt acggcttcgt gcgggcctgc ctgcgccggc
     4801 tggtgccccc aggcctctgg ggctccaggc acaacgaacg ccgcttcctc aggaacacca
     4861 agaagttcat ctccctgggg aagcatgcca agctctcgct gcaggagctg acgtggaaga
     4921 tgagcgtgcg gggctgcgct tggctgcgca ggagcccagg ggttggctgt gttccggccg
     4981 cagagcaccg tctgcgtgag gagatcctgg ccaagttcct gcactggctg atgagtgtgt
     5041 acgtcgtcga gctgctcagg tctttctttt atgtcacgga gaccacgttt caaaagaaca
     5101 ggctcttttt ctaccggaag agtgtctgga gcaagttgca aagcattgga atcagacagc
     5161 acttgaagag ggtgcagctg cgggagctgt cggaagcaga ggtcaggcag catcgggaag
     5221 ccaggcccgc cctgctgacg tccagactcc gcttcatccc caagcctgac gggctgcggc
     5281 cgattgtgaa catggactac gtcgtgggag ccagaacgtt ccgcagagaa aagagggccg
     5341 agcgtctcac ctcgagggtg aaggcactgt tcagcgtgct caactacgag cgggcgcggc
     5401 gccccggcct cctgggcgcc tctgtgctgg gcctggacga tatccacagg gcctggcgca
     5461 ccttcgtgct gcgtgtgcgg gcccaggacc cgccgcctga gctgtacttt gtcaaggtgg
     5521 atgtgacggg cgcgtacgac accatccccc aggacaggct cacggaggtc atcgccagca
     5581 tcatcaaacc ccagaacacg tactgcgtgc gtcggtatgc cgtggtccag aaggccgccc
     5641 atgggcacgt ccgcaaggcc ttcaagagcc acgtctctac cttgacagac ctccagccgt
     5701 acatgcgaca gttcgtggct cacctgcagg agaccagccc gctgagggat gccgtcgtca
     5761 tcgagcagag ctcctccctg aatgaggcca gcagtggcct cttcgacgtc ttcctacgct
     5821 tcatgtgcca ccacgccgtg cgcatcaggg gcaagtccta cgtccagtgc caggggatcc
     5881 cgcagggctc catcctctcc acgctgctct gcagcctgtg ctacggcgac atggagaaca
     5941 agctgtttgc ggggattcgg cgggacgggc tgctcctgcg tttggtggat gatttcttgt
     6001 tggtgacacc tcacctcacc cacgcgaaaa ccttcctcag gaccctggtc cgaggtgtcc
     6061 ctgagtatgg ctgcgtggtg aacttgcgga agacagtggt gaacttccct gtagaagacg
     6121 aggccctggg tggcacggct tttgttcaga tgccggccca cggcctattc ccctggtgcg
     6181 gcctgctgct ggatacccgg accctggagg tgcagagcga ctactccagc tatgcccgga
     6241 cctccatcag agccagtctc accttcaacc gcggcttcaa ggctgggagg aacatgcgtc
     6301 gcaaactctt tggggtcttg cggctgaagt gtcacagcct gtttctggat ttgcaggtga
     6361 acagcctcca gacggtgtgc accaacatct acaagatcct cctgctgcag gcgtacaggt
     6421 ttcacgcatg tgtgctgcag ctcccatttc atcagcaagt ttggaagaac cccacatttt
     6481 tcctgcgcgt catctctgac acggcctccc tctgctactc catcctgaaa gccaagaacg
     6541 cagggatgtc gctgggggcc aagggcgccg ccggccctct gccctccgag gccgtgcagt
     6601 ggctgtgcca ccaagcattc ctgctcaagc tgactcgaca ccgtgtcacc tacgtgccac
     6661 tcctggggtc actcaggaca gcccagacgc agctgagtcg gaagctcccg gggacgacgc
     6721 tgactgccct ggaggccgca gccaacccgg cactgccctc agacttcaag accatcctgg
     6781 actgagaatt cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa
     6841 ctaacttaag ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac
     6901 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     6961 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     7021 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     7081 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     7141 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     7201 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     7261 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     7321 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     7381 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     7441 cgataatacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     7501 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     7561 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     7621 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     7681 gcttgacatt ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7741 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7801 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     7861 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     7921 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     7981 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     8041 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     8101 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     8161 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     8221 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     8281 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     8341 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     8401 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     8461 gagggcaaag gaatagcccg ggcggggcgc gtctggaaca atcaacctct ggattacaaa
     8521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8881 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     8941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9061 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     9121 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     9181 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     9241 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     9301 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     9361 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     9421 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     9481 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     9541 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     9601 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     9661 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     9721 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     9781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9901 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     9961 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
    10021 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    10081 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    10141 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    10201 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10261 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10441 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10861 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11101 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    11161 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11221 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11281 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11341 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11401 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11461 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11521 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11581 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11641 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11701 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11761 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11821 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11881 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11941 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    12001 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12061 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    12121 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12181 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
    12241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    12301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12361 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    12421 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12481 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
