LOCUS Exported 6692 bp ds-DNA circular SYN 25-JUN-2021 DEFINITION mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells. ACCESSION . VERSION . KEYWORDS pcDNA3.1(+)-C-6His-mEsrra SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6692) TITLE Bacterial expression plasmids for production of mEsrra protein JOURNAL Unpublished REFERENCE 2 (bases 1 to 6692) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6692 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2246..2263 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(2283..2300) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2289..2513 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2559..2987 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2646..2665) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2856..2877 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2996..3016) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3001..3330 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3181..3316 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3243..3262 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3397..4191 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3451..3470) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4061..4080 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4365..4486 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4402..4421) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4456..4475 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4535..4551) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4535..4551) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4548..4570) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4559..4575 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4583..4613) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4628..4649 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4766..4783) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4937..5525) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5017..5036) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5696..6556) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6319..6338 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6557..6661) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgaattc tgcagatatc cagcacagtg 961 gcggccgctc gagatgtcca gccaggtggt gggcatcgag cctctctaca tcaaggcaga 1021 gccagccagt cctgacagtc caaagggttc ctcagagact gagactgaac ccccggtgac 1081 cctggcctct ggtccagctc cagcccgctg ccttccaggg cacaaggagg aggaggatgg 1141 ggagggggca gggtctggtg agcagggcag tgggaagcta gtgctcagct ctctacccaa 1201 acgcctctgc ctggtctgtg gggatgtggc ctctggctac cactacggtg tggcatcctg 1261 tgaggcctgc aaagccttct tcaagaggac catccagggg agcatcgagt acagctgtcc 1321 ggcctccaat gagtgtgaga tcaccaagcg gagacgcaag gcctgtcagg cctgccgctt 1381 caccaagtgc ctgcgggtgg gcatgctcaa ggagggtgtg cgtctggacc gtgtccgcgg 1441 cggacggcag aagtacaaac ggcggccaga ggtggaccct ttgcctttcc cgggcccctt 1501 ccctgctgga cctctggcag tagctggagg acccaggaag acagccccag tgaacgctct 1561 ggtgtcgcat ctgctggtgg ttgaacctga gaagctgtac gccatgcctg acccagcaag 1621 ccccgatgga cacctccccg ctgtggccac tctctgtgac ctttttgatc gagagatagt 1681 ggtcaccatc agctgggcca agagcatccc aggcttctcc tcactgtcac tgtctgacca 1741 gatgtcagta ctgcagagtg tgtggatgga agtgctggtg ctgggtgtgg cccagcgctc 1801 actgccactg caggatgagc tggcctttgc tgaggacctg gtcctagatg aagagggggc 1861 acgggcagct ggcctggggg atctgggggc agccctgctg cagctggttc ggcgactgca 1921 agctcttcgg ctggagcggg aggagtacgt cctgctgaaa gctctggccc ttgccaattc 1981 tgactctgtg cacattgaag atgctgaggc tgtggagcag ctgcgcgaag ccctgcatga 2041 ggccctgctg gagtatgaag ctggccgggc tggccctgga gggggtgctg agcggaggcg 2101 tgcaggcagg ctgctgctta cgctgccact cctccgccag acagcaggca aagtcctggc 2161 ccatttctat ggggtgaagc tggagggcaa ggtgcccatg cacaagctgt ttttggaaat 2221 gcttgaggcc atgatggacg ggccccatca tcaccatcac cattgataaa cccgctgatc 2281 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 2341 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 2401 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 2461 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 2521 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 2581 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 2641 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 2701 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 2761 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 2821 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 2881 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 2941 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 3001 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 3061 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 3121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 3181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 3241 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 3301 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 3361 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 3421 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 3481 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 3541 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 3601 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 3661 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 3721 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 3781 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 3841 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 3901 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 3961 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 4021 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 4081 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 4141 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 4201 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 4261 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 4321 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 4381 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 4441 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 4501 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 4561 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 4621 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4681 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4741 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4801 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4861 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4921 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4981 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 5041 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 5101 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5161 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5221 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5281 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5341 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 5401 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5461 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5521 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5581 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5641 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5701 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5761 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 5821 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5881 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5941 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6001 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6061 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6121 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6181 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6241 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6301 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6361 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6421 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6481 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 6541 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 6601 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 6661 gcacatttcc ccgaaaagtg ccacctgacg tc //