LOCUS       Exported                 887 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pENTR5'_ubi:loxP-EGFP-loxP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI
  TITLE     Ubiquitous transgene expression and Cre-based recombination driven 
            by the ubiquitin promoter in zebrafish.
  JOURNAL   Development. 2011 Jan . 138(1):169-77.
  PUBMED    21138979
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      13..22
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(29..62)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 cgcccttgct caccatggtg gcgaccggat aacttcgtat aatgtatgct atacgaagtt
       61 ataagcttgg atccctgtaa acaaattcaa agtaagatta gcaatcacac atttccattt
      121 aaaaaatact acaatgttct agccaataat gtatgttttg cacttctccc atgttaaacg
      181 agatagttca cccgaaaatg aaattttact ttgcatttgg tcacccttaa atgttacaaa
      241 cctttaaaat aatagaaaac tattgactat acttgtagag aaaaataaat actatccaaa
      301 acaatggctg acagtggact atagaatgca ttttacctgc agcaaaattg tccaaatgat
      361 cctatatgaa aatttagggt cttacctagt gaaatgatag ctcattttgc acaagctctg
      421 agatttggct ttgtgcatca tgaaatctaa ttatgtcatg catttccaaa gcattggcca
      481 ttttaaacaa actgcacatc tgtgcagttt acattgtttt aaaataaaaa ataaaaaatt
      541 aagaaataaa gaataataat gttccacaac attacattag gtagcagatt ggcagagaac
      601 caaagggtgg ggccagtaag gaaggacttc tacacagtaa ggaaacgctg aaattcaact
      661 taatcactca gtcatttaaa aataaatact tttatttttt ttatttttaa aggtctaatg
      721 gtttcattag ataagactgt tattccttga ctgggatcat ttagaaccat ttgaagccac
      781 atattgcatt ttggaatttt aaactaacaa gctccacaat atagagaaaa aaatcttgaa
      841 actttttcaa aaacaatttc ttcattaatg atgaaaaaaa gggatca
//
