LOCUS Exported 7082 bp ds-DNA circular SYN 30-JUN-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS shCTNNB1 # 2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7082) AUTHORS Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI, Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA, Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, Goessling W, Hahn WC TITLE beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex for Survival and Tumorigenesis. JOURNAL Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026. Epub 2012 Dec 13. PUBMED 23245941 REFERENCE 2 (bases 1 to 7082) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7082 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 128..147 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 381..398 /label=L4440 /note="L4440 vector, forward primer" protein_bind 515..536 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 551..581 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 589..605 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 594..616 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 613..629 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 613..629 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 648..668 /label=T3 /note="T3 promoter, forward primer" promoter 650..668 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 696..922 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 923..1103 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1150..1275 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1768..2001 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2186..2230 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2528..2768 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 2528..2548 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 2699..2718 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_feature 2872..2989 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 3038..3548 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 3467..3485 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 3570..4169 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3570..3589) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4066..4086 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 4297..4530 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4602..4723 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4639..4658) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4693..4712 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4763..4898 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4825..4844 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4918..4937) /label=T7 /note="T7 promoter, forward primer" promoter complement(4919..4937) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4947..4964) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4947..4963) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4956..4978) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5105..5560 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5192..5211) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5402..5423 /label=F1ori-F /note="F1 origin, forward primer" promoter 5586..5690 /gene="bla" /label=AmpR promoter CDS 5691..6551 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5909..5928) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6722..227 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 61 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 481 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 541 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 601 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc 661 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta 721 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg 781 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac 841 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa 901 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct 961 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 1021 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 1081 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa 1141 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag 1201 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat 1261 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg 1321 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag 1381 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata 1441 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat 1501 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct 1561 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct 1621 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata 1681 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt 1741 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc 1801 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc 1861 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt 1921 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata 1981 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac 2041 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac 2101 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat 2161 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg 2221 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat 2281 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa 2341 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg 2401 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat 2461 tcgattagtg aacggatctc gacggtatcg atcacgagac tagcctcgag cggccgcccc 2521 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2581 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2641 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2701 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2761 gaaaggacga aacaccgggc ttggaatgag actgctgatc tcgagatcag cagtctcatt 2821 ccaagctttt taattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag 2881 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 2941 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta 3001 cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc 3061 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca 3121 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct 3181 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag 3241 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta 3301 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg 3361 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg 3421 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct 3481 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct 3541 ctccccaggg ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg 3601 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 3661 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 3721 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 3781 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 3841 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 3901 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 3961 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 4021 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 4081 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 4141 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg 4201 aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg 4261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 4321 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 4381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 4441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 4501 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 4561 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 4621 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 4681 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 4741 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4861 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc 4921 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa 4981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 5041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 5101 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 5161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 5221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 5281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 5341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 5401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 5461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 5521 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 5581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 5641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 5701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 5761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 5821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 5881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 5941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 6001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 6061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 6121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 6181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 6241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 6301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 6361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 6421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 6481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 6541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 6601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 6661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 6721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 6781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 6841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 6901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 6961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 7021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 7081 cc //