LOCUS       Exported                8511 bp ds-DNA     circular SYN 20-JUL-2021
DEFINITION  Mammalian expression of un-tagged human androgen receptor 
            phosphorylation mutant: AR-Ser256Ala (AR-S256A) .
ACCESSION   .
VERSION     .
KEYWORDS    pCIneo-AR-S256A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Chen S, Xu Y, Yuan X, Bubley GJ, Balk SP
  TITLE     Androgen receptor phosphorylation and stabilization in prostate 
            cancer by cyclin-dependent kinase 1.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15969-74. doi: 
            10.1073/pnas.0604193103. Epub 2006 Oct 16.
  PUBMED    17043241
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4180..4197)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    4215..4336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4226..4245)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4280..4299
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4522..4977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4609..4628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4819..4840
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5094..5451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(5117..5137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5302..5437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5364..5383
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5502..6296
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5556..5575)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6166..6185
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6360..6408
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6449..6468)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6568..6590
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6628..6646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6714..6818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6819..7679
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7037..7056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7850..8438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8339..8358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc gtttaaactt aagcttggta ccgagctcgg atccatggaa gtgcagttag
     1141 ggctgggaag ggtctaccct cggccgccgt ccaagaccta ccgaggagct ttccagaatc
     1201 tgttccagag cgtgcgcgaa gtgatccaga acccgggccc caggcaccca gaggccgcga
     1261 gcgcagcacc tcccggcgcc agtttgctgc tgctgcagca gcagcagcag cagcagcagc
     1321 agcagcagca gcagcaagag actagcccca ggcagcagca gcagcagcag ggtgaggatg
     1381 gttctcccca agcccatcgt agaggcccca caggctacct ggtcctggat gaggaacagc
     1441 aaccttcaca gccgcagtcg gccctggagt gccaccccga gagaggttgc gtcccagagc
     1501 ctggagccgc cgtggccgcc agcaaggggc tgccgcagca gctgccagca cctccggacg
     1561 aggatgactc agctgcccca tccacgttgt ccctgctggg ccccactttc cccggcttaa
     1621 gcagctgctc cgctgacctt aaagacatcc tgagcgaggc cagcaccatg caactccttc
     1681 agcaacagca gcaggaagca gtatccgaag gcagcagcag cgggagagcg agggaggcct
     1741 cgggggctcc cacttcctcc aaggacaatt acttaggggg cacttcgacc atttctgaca
     1801 acgccaagga gttgtgtaag gcagtgtcgg tgtccatggg cctgggtgtg gaggcgttgg
     1861 agcatctggc tccaggggaa cagcttcggg gggattgcat gtacgcccca cttttgggag
     1921 ttccacccgc tgtgcgtccc actccttgtg ccccattggc cgaatgcaaa ggttctctgc
     1981 tagacgacag cgcaggcaag agcactgaag atactgctga gtattcccct ttcaagggag
     2041 gttacaccaa agggctagaa ggcgagagcc taggctgctc tggcagcgct gcagcaggga
     2101 gctccgggac acttgaactg ccgtctaccc tgtctctcta caagtccgga gcactggacg
     2161 aggcagctgc gtaccagagt cgcgactact acaactttcc actggctctg gccggaccgc
     2221 cgccccctcc gccgcctccc catccccacg ctcgcatcaa gctggagaac ccgctggact
     2281 acggcagcgc ctgggcggct gcggcggcgc agtgccgcta tggggacctg gcgagcctgc
     2341 atggcgcggg tgcagcggga cccggttctg ggtcaccctc agccgccgct tcctcatcct
     2401 ggcacactct cttcacagcc gaagaaggcc agttgtatgg accgtgtggt ggtggtgggg
     2461 gtggtggtgg cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg
     2521 gcggcggcga ggcgggagct gtagccccct acggctacac tcggccccct caggggctgg
     2581 cgggccagga aagcgacttc accgcacctg atgtgtggta ccctggcggc atggtgagca
     2641 gagtgcccta tcccagtccc acttgtgtca aaagcgaaat gggcccctgg atggatagct
     2701 actccggacc ttacggggac atgcgtttgg agactgccag ggaccatgtt ttgcccattg
     2761 actattactt tccaccccag aagacctgcc tgatctgtgg agatgaagct tctgggtgtc
     2821 actatggagc tctcacatgt ggaagctgca aggtcttctt caaaagagcc gctgaaggga
     2881 aacagaagta cctgtgcgcc agcagaaatg attgcactat tgataaattc cgaaggaaaa
     2941 attgtccatc ttgtcgtctt cggaaatgtt atgaagcagg gatgactctg ggagcccgga
     3001 agctgaagaa acttggtaat ctgaaactac aggaggaagg agaggcttcc agcaccacca
     3061 gccccactga ggagacaacc cagaagctga cagtgtcaca cattgaaggc tatgaatgtc
     3121 agcccatctt tctgaatgtc ctggaagcca ttgagccagg tgtagtgtgt gctggacacg
     3181 acaacaacca gcccgactcc tttgcagcct tgctctctag cctcaatgaa ctgggagaga
     3241 gacagcttgt acacgtggtc aagtgggcca aggccttgcc tggcttccgc aacttacacg
     3301 tggacgacca gatggctgtc attcagtact cctggatggg gctcatggtg tttgccatgg
     3361 gctggcgatc cttcaccaat gtcaactcca ggatgctcta cttcgcccct gatctggttt
     3421 tcaatgagta ccgcatgcac aagtcccgga tgtacagcca gtgtgtccga atgaggcacc
     3481 tctctcaaga gtttggatgg ctccaaatca ccccccagga attcctgtgc atgaaagcac
     3541 tgctactctt cagcattatt ccagtggatg ggctgaaaaa tcaaaaattc tttgatgaac
     3601 ttcgaatgaa ctacatcaag gaactcgatc gtatcattgc atgcaaaaga aaaaatccca
     3661 catcctgctc aagacgcttc taccagctca ccaagctcct ggactccgtg cagcctattg
     3721 cgagagagct gcatcagttc acttttgacc tgctaatcaa gtcacacatg gtgagcgtgg
     3781 actttccgga aatgatggca gagatcatct ctgtgcaagt gcccaagatc ctttctggga
     3841 aagtcaagcc catctatttc cacacccagt gaagcattgg aaaccctatt tccccacccc
     3901 agctcatgcc ccctttcaga tgtcttctgc ctgttataac tctgcactac tcctctgcag
     3961 tgccttgggg aatttcctct attgatgtac agtctgtcat gaacatgttc ctgaattcta
     4021 tttgctgggc tttttttttc tctttctctc ctttcttttt cttcttccct ccctatctaa
     4081 ccctcccatg gcaccttcag actttgcttc ccattgtggc tcctatctgt gttttgaatg
     4141 gtgttgtatg cctttatcca gcacagtggc ggccgcttcc ctttagtgag ggttaatgct
     4201 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4261 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4321 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4381 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg
     4441 atcgatccgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4501 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4561 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4621 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4681 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4741 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4801 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4861 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4921 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     4981 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc
     5041 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
     5101 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5161 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5221 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5281 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5341 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5401 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat
     5461 tcttctgaca caacagtctc gaacttaagg ctagagccac catgattgaa caagatggat
     5521 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5581 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5641 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5701 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5761 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5821 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5881 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5941 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6001 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6061 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6121 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6181 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6241 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6301 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca
     6361 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga
     6421 taaggatccg cgtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6481 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6541 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6601 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     6661 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8461 tacggttcct ggccttttgc tggccttttg ctcacatggc tcgacagatc t
//
