LOCUS       Exported                2820 bp ds-DNA     linear   SYN 30-SEP-2021
DEFINITION  Bacterial expression of PARP1 lacking Zn1 domain but containing 
            L713F gain-of-function mutation.
ACCESSION   .
VERSION     .
KEYWORDS    pET28_6xHis-PARP1delZn1-L713F
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn
            MP, Unruh JR, Florens L, Conaway RC, Conaway JW
  TITLE     Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 
            10.1073/pnas.2107277118.
  PUBMED    34465625
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgaaaggcc aggatggaat tggtagcaag gcagagaaga ctctgggtga ctttgcagca
      121 gagtatgcca agtccaacag aagtacgtgc aaggggtgta tggagaagat agaaaagggc
      181 caggtgcgcc tgtccaagaa gatggtggac ccggagaagc cacagctagg catgattgac
      241 cgctggtacc atccaggctg ctttgtcaag aacagggagg agctgggttt ccggcccgag
      301 tacagtgcga gtcagctcaa gggcttcagc ctccttgcta cagaggataa ggaagccctg
      361 aagaagcagc tcccaggagt caagagtgaa ggaaagagaa aaggcgatga ggtggatgga
      421 gtggatgaag tggcgaagaa gaaatctaaa aaagaaaaag acaaggatag taagcttgaa
      481 aaagccctaa aggctcagaa cgacctgatc tggaacatca aggacgagct aaagaaagtg
      541 tgttcaacta atgacctgaa ggagctactc atcttcaaca agcagcaagt gccttctggg
      601 gagtcggcga tcttggaccg agtagctgat ggcatggtgt tcggtgccct ccttccctgc
      661 gaggaatgct cgggtcagct ggtcttcaag agcgatgcct attactgcac tggggacgtc
      721 actgcctgga ccaagtgtat ggtcaagaca cagacaccca accggaagga gtgggtaacc
      781 ccaaaggaat tccgagaaat ctcttacctc aagaaattga aggttaaaaa gcaggaccgt
      841 atattccccc cagaaaccag cgcctccgtg gcggccacgc ctccgccctc cacagcctcg
      901 gctcctgctg ctgtgaactc ctctgcttca gcagataagc cattatccaa catgaagatc
      961 ctgactctcg ggaagctgtc ccggaacaag gatgaagtga aggccatgat tgagaaactc
     1021 ggggggaagt tgacggggac ggccaacaag gcttccctgt gcatcagcac caaaaaggag
     1081 gtggaaaaga tgaataagaa gatggaggaa gtaaaggaag ccaacatccg agttgtgtct
     1141 gaggacttcc tccaggacgt ctccgcctcc accaagagcc ttcaggagtt gttcttagcg
     1201 cacatcttgt ccccttgggg ggcagaggtg aaggcagagc ctgttgaagt tgtggcccca
     1261 agagggaagt caggggctgc gctctccaaa aaaagcaagg gccaggtcaa ggaggaaggt
     1321 atcaacaaat ctgaaaagag aatgaaatta actcttaaag gaggagcagc tgtggatcct
     1381 gattctggac tggaacactc tgcgcatgtc ctggagaaag gtgggaaggt cttcagtgcc
     1441 acccttggcc tggtggacat cgttaaagga accaactcct actacaagct ccagcttctg
     1501 gaggacgaca aggaaaacag gtattggata ttcaggtcct ggggccgtgt gggtacggtg
     1561 atcggtagca acaaactgga acagatgccg tccaaggagg atgccattga gcacttcatg
     1621 aaattatatg aagaaaaaac cgggaacgct tggcactcca aaaatttcac gaagtatccc
     1681 aaaaagttct accccctgga gattgactat ggccaggatg aagaggcagt gaagaagctg
     1741 acagtaaatc ctggcaccaa gtccaagctc cccaagccag ttcaggacct catcaagatg
     1801 atctttgatg tggaaagtat gaagaaagcc atggtggagt atgagatcga ccttcagaag
     1861 atgcccttgg ggaagctgag caaaaggcag atccaggccg catactccat cttcagtgag
     1921 gtccagcagg cggtgtctca gggcagcagc gactctcaga tcctggatct ctcaaatcgc
     1981 ttttacaccc tgatccccca cgactttggg atgaagaagc ctccgctcct gaacaatgca
     2041 gacagtgtgc aggccaaggc ggaaatgctt gacaacctgc tggacatcga ggtggcctac
     2101 agtctgctca ggggagggtc tgatgatagc agcaaggatc ccatcgatgt caactatgag
     2161 aagctcaaaa ctgacattaa ggtggttgac agagattctg aagaagccga gatcatcagg
     2221 aagtatgtta agaacactca tgcaaccaca cacaatgcgt atgacttgga agtcatcgat
     2281 atctttaaga tagagcgtga aggcgaatgc cagcgttaca agccctttaa gcagcttcat
     2341 aaccgaagat tgctgtggca cgggtccagg accaccaact ttgctgggat cctgtcccag
     2401 ggtcttcgga tagccccgcc tgaagcgccc gtgacaggct acatgtttgg taaagggatc
     2461 tatttcgctg acatggtctc caagagtgcc aactactgcc atacgtctca gggagaccca
     2521 ataggcttaa tcctgttggg agaagttgcc cttggaaaca tgtatgaact gaagcacgct
     2581 tcacatatca gcaagttacc caagggcaag cacagtgtca aaggtttggg caaaactacc
     2641 cctgatcctt cagctaacat tagtctggat ggtgtagacg ttcctcttgg gaccgggatt
     2701 tcatctggtg tgaatgacac ctctctacta tataacgagt acattgtcta tgatattgct
     2761 caggtaaatc tgaagtatct gctgaaactg aaattcaatt ttaagacctc cctgtggtaa
//
