LOCUS       Exported               13989 bp ds-DNA     circular SYN 28-JUL-2021
DEFINITION  Lentiviral expression vector for human direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    pLL3.7-CAG-hMGT-Ef1a-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Garbutt TA, Zhou Y, Keepers B, Liu J, Qian L
  TITLE     An Optimized Protocol for Human Direct Cardiac Reprogramming.
  JOURNAL   STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100010. Epub
            2020 Jun 3.
  PUBMED    32728671
REFERENCE   2  (bases 1 to 13989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1427..1483
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2831..2884
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        4512..4723
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4736..5004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5031..5630
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5031..5050)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5527..5547
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5637..6225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5690..5710)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6108..6119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6299..6532
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6604..6725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6641..6660)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6695..6714
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6765..6900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6920..6939)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6921..6939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6962..7320)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7002..7023)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7214..7233
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7347..7451
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7476..7496)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7520..7730
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7542..7738
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7589..7724
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7773..8567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7827..7846)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8437..8456
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8755..8774)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8799..8846
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9175..9763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9664..9683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9938..10241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10243..10441
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10396..10416
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10459..10639
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10686..10811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11308..11541
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11726..11770
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12068..12185
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        12280..12659
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12661..12937
                     /label=chicken beta-actin promoter
     intron          12938..13954
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     13878..13900
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     13962..13981
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ttaattaacc atcgataccg ccatggggag aaaaaagatt cagattacga ggattatgga
       61 tgaacgtaac agacaggtga catttacaaa gaggaaattt gggttgatga agaaggctta
      121 tgagctgagc gtgctgtgtg actgtgagat tgcgctgatc atcttcaaca gcaccaacaa
      181 gctgttccag tatgccagca ccgacatgga caaagtgctt ctcaagtaca cggagtacaa
      241 cgagccgcat gagagccgga caaactcaga catcgtggag gcattgaaca agaaagaaaa
      301 caaaggctgt gaaagccccg atcccgactc ctcttatgca ctcaccccac gcactgaaga
      361 aaaatacaaa aaaattaatg aagaatttga taatatgatc aagagtcata aaattcctgc
      421 tgttccacct cccaacttcg agatgccagt ctccatccca gtgtccagcc acaacagttt
      481 ggtgtacagc aaccctgtca gctcactggg aaaccccaac ctattgccac tggctcaccc
      541 ttctctgcag aggaatagta tgtctcctgg tgtaacacat cgacctccaa gtgcaggtaa
      601 cacaggtggt ctgatgggtg gagacctcac gtctggtgca ggcaccagtg cagggaacgg
      661 gtatggcaat ccccgaaact caccaggtct gctggtctca cctggtaact tgaacaagaa
      721 tatgcaagca aaatctcctc ccccaatgaa tttaggaatg aataaccgta aaccagatct
      781 ccgagttctt attccaccag gcagcaagaa tacgatgcca tcagtgaatc aaaggataaa
      841 taactcccag tcggctcagt cattggctac cccagtggtt tccgtagcaa ctcctacttt
      901 accaggacaa ggaatgggag gatatccatc agccatttca acaacatatg gtaccgagta
      961 ctctctgagt agtgcagacc tgtcatctct gtctgggttt aacaccgcca gcgctcttca
     1021 ccttggttca gtaactggct ggcaacagca acacctacat aacatgccac catctgccct
     1081 cagtcagttg ggagcttgca ctagcactca tttatctcag agttcaaatc tctccctgcc
     1141 ttctactcaa agcctcaaca tcaagtcaga acctgtttct cctcctagag accgtaccac
     1201 caccccttcg agatacccac aacacacgcg ccacgaggcg gggagatctc ctgttgacag
     1261 cttgagcagc tgtagcagtt cgtacgacgg gagcgaccga gaggatcacc ggaacgaatt
     1321 ccactccccc attggactca ccagaccttc gccggacgaa agggaaagtc cctcagtcaa
     1381 gcgcatgcga ctttctgaag gatgggcaac agctagcggc agcggcgcca caaacttctc
     1441 tctgctaaag caagcaggtg atgttgaaga aaaccccggg cctactagta tgtatcagag
     1501 cttggccatg gccgccaacc acgggccgcc ccccggtgcc tacgaggcgg gcggccccgg
     1561 cgccttcatg cacggcgcgg gcgccgcgtc ctcgccagtc tacgtgccca caccgcgggt
     1621 gccctcctcc gtgctgggcc tgtcctacct ccagggcgga ggcgcgggct ctgcgtccgg
     1681 aggcgcctcg ggcggcagct ccggtggggc cgcgtctggt gcggggcccg ggacccagca
     1741 gggcagcccg ggatggagcc aggcgggagc cgacggagcc gcttacaccc cgccgccggt
     1801 gtcgccgcgc ttctccttcc cggggaccac cgggtccctg gcggccgccg ccgccgctgc
     1861 cgcggcccgg gaagctgcgg cctacagcag tggcggcgga gcggcgggtg cgggcctggc
     1921 gggccgcgag cagtacgggc gcgccggctt cgcgggctcc tactccagcc cctacccggc
     1981 ttacatggcc gacgtgggcg cgtcctgggc cgcagccgcc gccgcctccg ccggcccctt
     2041 cgacagcccg gtcctgcaca gcctgcccgg ccgggccaac ccggccgccc gacaccccaa
     2101 tctcgatatg tttgacgact tctcagaagg cagagagtgt gtcaactgtg gggctatgtc
     2161 caccccgctc tggaggcgag atgggacggg tcactatctg tgcaacgcct gcggcctcta
     2221 ccacaagatg aacggcatca accggccgct catcaagcct cagcgccggc tgtccgcctc
     2281 ccgccgagtg ggcctctcct gtgccaactg ccagaccacc accaccacgc tgtggcgccg
     2341 caatgcggag ggcgagcctg tgtgcaatgc ctgcggcctc tacatgaagc tccacggggt
     2401 ccccaggcct cttgcaatgc ggaaagaggg gatccaaacc agaaaacgga agcccaagaa
     2461 cctgaataaa tctaagacac cagcagctcc ttcaggcagt gagagccttc ctcccgccag
     2521 cggtgcttcc agcaactcca gcaacgccac caccagcagc agcgaggaga tgcgtcccat
     2581 caagacggag cctggcctgt catctcacta cgggcacagc agctccgtgt cccagacgtt
     2641 ctcagtcagt gcgatgtctg gccatgggcc ctccatccac cctgtcctct cggccctgaa
     2701 gctctcccca caaggctatg cgtctcccgt cagccagtct ccacagacca gctccaagca
     2761 ggactcttgg aacagcctgg tcttggccga cagtcacggg gacataatca ctgcgaagct
     2821 tggctccggc gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg
     2881 cccactcgac atggccgacg cagacgaggg ctttggcctg gcgcacacgc ctctggagcc
     2941 tgacgcaaaa gacctgccct gcgattcgaa acccgagagc gcgctcgggg cccccagcaa
     3001 gtccccgtcg tccccgcagg ccgccttcac ccagcagggc atggagggaa tcaaagtgtt
     3061 tctccatgaa agagaactgt ggctaaaatt ccacgaagtg ggcacggaaa tgatcataac
     3121 caaggctgga aggcggatgt ttcccagtta caaagtgaag gtgacgggcc ttaatcccaa
     3181 aacgaagtac attcttctca tggacattgt acctgccgac gatcacagat acaaattcgc
     3241 agataataaa tggtctgtga cgggcaaagc tgagcccgcc atgcctggcc gcctgtacgt
     3301 gcacccagac tcccccgcca ccggggcgca ttggatgagg cagctcgtct ccttccagaa
     3361 actcaagctc accaacaacc acctggaccc atttgggcat attattctaa attccatgca
     3421 caaataccag cctagattac acatcgtgaa agcggatgaa aataatggat ttggctcaaa
     3481 aaatacagcg ttctgcactc acgtctttcc tgagactgcg tttatagcag tgacttccta
     3541 ccagaaccac aagatcacgc aattaaagat tgagaataat ccctttgcca aaggatttcg
     3601 gggcagtgat gacatggagc tgcacagaat gtcaagaatg caaagtaaag aatatcccgt
     3661 ggtccccagg agcaccgtga ggcaaaaagt ggcctccaac cacagtcctt tcagcagcga
     3721 gtctcgagct ctctccacct catccaattt ggggtcccaa taccagtgtg agaatggtgt
     3781 ttccggcccc tcccaggacc tcctgcctcc acccaaccca tacccactgc cccaggagca
     3841 tagccaaatt taccattgta ccaagaggaa agaggaagaa tgttccacca cagaccatcc
     3901 ctataagaag ccctacatgg agacatcacc cagtgaagaa gattccttct accgctctag
     3961 ctatccacag cagcagggcc tgggtgcctc ctacaggaca gagtcggcac agcggcaagc
     4021 ttgcatgtat gccagctctg cgccccccag cgagcctgtg cccagcctag aggacatcag
     4081 ctgcaacacg tggccaagca tgccttccta cagcagctgc accgtcacca ccgtgcagcc
     4141 catggacagg ctaccctacc agcacttctc cgctcacttc acctcggggc ccctggtccc
     4201 tcggctggct ggcatggcca accatggctc cccacagctg ggagagggaa tgttccagca
     4261 ccagacctcc gtggcccacc agcctgtggt caggcagtgt gggcctcaga ctggcctgca
     4321 gtcccctggc acccttcagc cccctgagtt cctctactct catggcgtgc caaggactct
     4381 atcccctcat cagtaccact ctgtgcacgg agttggcatg gtgccagagt ggagcgacaa
     4441 tagctaatta attaaggccg gccctcgagg ttggccgcga aggatctgcg atcgctccgg
     4501 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4561 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4621 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4681 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     4741 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     4801 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     4861 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     4921 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     4981 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt
     5041 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     5101 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     5161 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     5221 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     5281 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     5341 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     5401 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5461 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5521 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5581 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc
     5641 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5701 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5761 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5821 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5881 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5941 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6001 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6061 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6121 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6181 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     6241 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6301 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6361 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6421 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6481 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6541 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6601 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6661 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6721 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6781 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6841 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6901 tagggacgta cccaattcgc cctatagtga gtcgtattag catgtgagca aaaggccagc
     6961 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7021 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7081 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     7141 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     7201 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     7261 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     7321 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7381 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7441 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     7501 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7561 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7621 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7681 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     7741 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     7801 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7861 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7921 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     7981 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     8041 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8101 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8161 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     8221 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8281 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     8341 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8401 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8461 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8521 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8581 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8641 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8701 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     8761 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     8821 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     8881 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     8941 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     9001 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     9061 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9841 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
     9901 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     9961 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
    10021 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
    10081 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
    10141 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
    10201 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
    10261 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
    10321 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
    10381 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
    10441 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10501 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10561 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    10621 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
    10681 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
    10741 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
    10801 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
    10861 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
    10921 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
    10981 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
    11041 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
    11101 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
    11161 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    11221 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    11281 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    11341 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    11401 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    11461 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
    11521 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
    11581 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
    11641 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    11701 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    11761 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    11821 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    11881 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
    11941 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
    12001 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
    12061 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    12121 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    12181 attttcgggt ttattacagg gacagcagag atccagtttg gttagtaccg ggcccgctct
    12241 agccctagat ttaacccaac agaaggctcg acgcgtgtcg acattgatta ttgactagtt
    12301 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    12361 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    12421 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    12481 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    12541 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    12601 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    12661 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
    12721 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
    12781 ncgcgcgcca ggcggggcgg ggsggggsgr ggggsggggs ggggcgaggc ggagaggtgc
    12841 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
    12901 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
    12961 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
    13021 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
    13081 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
    13141 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
    13201 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
    13261 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
    13321 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
    13381 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
    13441 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
    13501 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
    13561 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
    13621 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
    13681 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
    13741 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
    13801 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
    13861 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
    13921 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
    13981 ggtttaaac
//
