LOCUS Exported 9022 bp ds-DNA circular SYN 16-AUG-2021 DEFINITION Retroviral expression vector for murine direct cardiac reprogramming. ACCESSION . VERSION . KEYWORDS pmx-MiGT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9022) AUTHORS Wang L, Huang P, Near D, Ravi K, Xu Y, Liu J, Qian L TITLE Isoform Specific Effects of Mef2C during Direct Cardiac Reprogramming. JOURNAL Cells. 2020 Jan 22;9(2). pii: cells9020268. doi: 10.3390/cells9020268. PUBMED 31979018 REFERENCE 2 (bases 1 to 9022) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9022 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1094..1510 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1440..1462 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1478..1494 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1520..1894 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1832..1848 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" CDS 3339..3395 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 4740..4793 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" LTR 6527..6996 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(7126..7143) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7297..7885) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7377..7396) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8049..8909) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8672..8691 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8910..9014) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa 1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac 1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa 1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc 1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt 1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc 1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc 1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa 1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca 1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca 1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg 1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac 1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc 1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat 1921 ccgccatggg gagaaaaaag attcagatta cgaggataat ggatgagcgt aacagacagg 1981 tgacttttac gaagaggaaa tttggattga tgaagaaggc ttatgagctg agcgtgctgt 2041 gtgactgtga gattgcactg atcatcttca acagcaccaa caagctgttc cagtacgcca 2101 gcactgacat ggataaggtg ttgctcaagt acaccgagta caacgagccg cacgagagcc 2161 ggacaaactc agacattgtg gagacattga gaaagaaggg cctcaatggc tgtgacagcc 2221 cagatcccga tgcagacgat tcagtaggtc acagccctga gtctgaggac aagtacagga 2281 aaattaacga agatattgat ctaatgatca gcaggcaaag attgtgtgct gttccacctc 2341 ccagctttga gatgccagtt accatcccag tgtccagcca taacagtttg gtgtacagca 2401 atcctgtcag cacactggga aaccccaatc ttctgccact ggcccacccg tctctgcaga 2461 ggaatagtat gtctcctggt gtaacacata gacctccaag tgcaggtaac acaggcggtc 2521 tgatgggcgg agatctgaca tccggtgcag gcaccagcgc agggaatgga tacggcaacc 2581 cccggaactc accaggcctg ctggtctcac ctggtaacct gaacaagaat atacaagcca 2641 aatctcctcc ccctatgaat ctaggaatga ataatcgtaa gccagatctc cgcgttctta 2701 tcccacctgg cagcaagaac acgatgccat cagtgaatca aaggataaat aactcccagt 2761 cggctcagtc attggctacc ccggtggttt ccgtagcaac tcctacttta ccaggacaag 2821 gaatgggagg atatccatca gccatttcaa caacatatgg tactgagtac tctctgagta 2881 gcgcagatct gtcatctctg tctggcttca acactgccag tgcgctccac ctcggctctg 2941 taactggctg gcagcagcag cacctacata acatgccgcc atctgccctc agtcagttgg 3001 gagcttgcac tagcactcat ttatctcaga gttcaaatct ctccctgcct tctactcaaa 3061 gcctcagcat caagtcagaa cctgtttctc ctcctagaga ccgtaccacc accccttcga 3121 gatacccaca acacaccacg cgccacgagg cggggaggtc tcctgttgac agcttgagca 3181 gctgtagcag ttcctacgat gggagcgacc gagaggatca ccggaacgaa ttccactccc 3241 ccattggact caccagacct tcgccggacg aaagggaaag tccttcagtc aagcgcatgc 3301 gactctctga aggatgggca acagctagcg gcagcggcgc cacgaacttc tctctgttaa 3361 agcaagcagg agatgttgaa gaaaaccccg ggcctgcatg catgtaccaa agcctggcca 3421 tggccgccaa ccacggcccc ccgcccggcg cctacgaagc aggtggccct ggcgccttca 3481 tgcacagcgc gggcgccgcg tcctcgcccg tctacgtgcc cactccgcgg gtgccgtcct 3541 ctgtgctggg cctgtcctac ctgcagggcg gtggcagtgc cgctgcagct ggaaccacct 3601 cgggtggcag ctccggggcc ggcccgtcgg gtgcagggcc tgggacccag cagggtagcc 3661 ctggctggag ccaagctgga gccgagggag ccgcctacac cccgccgccc gtgtccccgc 3721 gcttctcttt cccggggact actgggtccc tggcggccgc tgccgccgct gccgcagccc 3781 gggaagctgc agcctacggc agtggcggcg gggcggcggg cgctggtctg gctggccgag 3841 agcagtacgg gcgtccgggc ttcgccggct cctactccag cccctaccca gcctacatgg 3901 ccgacgtggg agcatcctgg gccgcagccg ctgccgcctc tgccggcccc ttcgacagcc 3961 cagtcctgca cagcctgcct ggacgggcca accctggaag acaccccaat ctcgatatgt 4021 ttgatgactt ctcagaaggc agagagtgtg tcaattgtgg ggccatgtcc accccactct 4081 ggaggcgaga tgggacggga cactacctgt gcaatgcctg tggcctctat cacaagatga 4141 acggcatcaa ccggcccctc attaagcctc agcgccgcct gtccgcttcc cgccgggtag 4201 gcctctcctg tgccaactgc cagactacca ccaccacgct gtggcgtcgt aatgccgagg 4261 gtgagcctgt atgtaatgcc tgcggcctct acatgaagct ccatggggtt cccaggcctc 4321 ttgcaatgcg gaaggagggg attcaaacca gaaaacggaa gcccaagaac ctgaataaat 4381 ctaagacgcc agcaggtcct gctggtgaga ccctccctcc ctccagtggt gcctccagcg 4441 gtaactccag caatgccact agcagcagca gcagcagtga agagatgcgc cccatcaaga 4501 cagagcccgg gctgtcatct cactatgggc acagcagctc catgtcccag acattcagta 4561 ctgtgtccgg ccacgggccc tccatccatc cagtgctgtc tgctctgaag ctgtccccac 4621 aaggctatgc atctcctgtc actcagacat cgcaggccag ctccaagcag gactcttgga 4681 acagcctggt cctggctgac agtcatgggg acataatcac cgcggcatgc ggctccggcg 4741 agggcagggg aagtcttcta acatgcgggg acgtggagga aaatcccggc ccactcgaga 4801 tggccgatac agatgagggc tttggcctgg cgcgcacgcc tctggagcct gattccaaag 4861 acaggtcttg cgattcgaaa cctgagagtg ctctgggggc tcccagcaag tctccatcat 4921 ccccgcaggc tgccttcacc cagcagggca tggaaggaat caaggtgttt cttcatgaac 4981 gtgaactgtg gctgaagttc cacgaagtgg gcacagagat gatcatcacc aaggcaggga 5041 ggagaatgtt tcctagttac aaagtgaagg tgactggcct taatcccaaa acgaagtata 5101 ttcttctcat ggatattgtt cccgcagacg accacagata taaatttgct gataacaaat 5161 ggtccgtaac tggcaaagca gagcctgcca tgccggggcg cctttacgtg cacccggact 5221 ccccagcaac cggagcccac tggatgcgac aacttgtctc cttccagaag ctcaaactca 5281 ccaacaacca cctggacccg tttggacaca ttatcctgaa ctccatgcac aaataccagc 5341 cccgattaca catcgtgaaa gcagacgaaa ataatgggtt cggttcaaag aacactgcgt 5401 tttgcaccca cgtcttcccg gagacagctt ttatcgctgt gacttcgtac cagaatcaca 5461 agatcacaca gctgaaaatt gagaacaacc ccttcgccaa aggctttcgg ggcagtgatg 5521 acctggagtt acacaggatg tctcggatgc aaagtaaaga gtatcctgtg gttcccagga 5581 gcacagtgag gcacaaagtc acctccaacc acagcccctt cagcagcgag acccgagctc 5641 tctccacctc atccaattta gggtcccagt accagtgtga gaatggtgtc tctggcccct 5701 cccaggacct tctgccccca cctaacccat acccactggc ccaggagcac agccaaattt 5761 accactgtac caagaggaaa gatgaggaat gttccagcac ggagcacccc tataagaagc 5821 cgtacatgga gacatccccc agcgaggaag acaccttcta tcgctcgggc tacccccagc 5881 agcagggcct gagtacctct tacaggacag agtcggccca gcggcaggcc tgcatgtatg 5941 ccagctccgc tccccccagc gagcccgtgc ctagcctgga ggacatcagc tgtaacacat 6001 ggcccagcat gccctcctat agcagctgta ccgtcaccac cgtgcagccc atggaccgtc 6061 ttccctacca gcacttctcc gctcatttca cctcggggcc cctggtccct cggttggctg 6121 gcatggccaa ccatggttct ccccagctcg gcgaagggat gtttcagcac cagacctcag 6181 tggcccatca gcctgtggtc aggcagtgcg ggcctcagac tggccttcag tctccgggcg 6241 gcctccagcc cccagagttt ctctacactc acggcgtgcc caggaccctg tccccccatc 6301 agtatcactc ggtacacggc gtcggcatgg tgccagagtg gagtgagaat agctaagtcg 6361 acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc 6421 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa 6481 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca 6541 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca 6601 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca 6661 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg 6721 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag 6781 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa 6841 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca 6901 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct 6961 ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat caaaattaat 7021 ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca 7081 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 7741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7921 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7981 cacctagatc cttttgcggc cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg 8041 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 8101 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 8161 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 8221 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 8281 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 8341 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 8401 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 8461 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 8521 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 8581 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 8641 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 8701 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 8761 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 8821 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 8881 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 8941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 9001 ataggggttc cgcgcacatt tc //