LOCUS Exported 6891 bp ds-DNA circular SYN 28-JUL-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBABEpuro U3-hTR-500 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6891) AUTHORS Wong JM, Collins K TITLE Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita. JOURNAL Genes Dev. 2006 Oct 15. 20(20):2848-58. PUBMED 17015423 REFERENCE 2 (bases 1 to 6891) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 28, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6891 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..470 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 535..734 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 935..1351 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1281..1303 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1319..1335 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(1409..1429) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1414..1743 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1594..1729 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1656..1675 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 1753..2352 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(1753..1772) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 2249..2269 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(3825..3841) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3825..3841) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(3873..3892) /label=T7 /note="T7 promoter, forward primer" promoter complement(3874..3892) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3899..3916) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3899..3915) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3908..3930) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(4144..4163) /label=F1ori-R /note="F1 origin, reverse primer" LTR 4378..4847 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4977..4994) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5148..5736) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5228..5247) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5896..6756) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6519..6538 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6757..6861) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat 301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg 361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct 421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct 481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg 541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct 601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga 661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg 721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct 781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg 841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg 901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag 961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt 1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg 1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc 1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc 1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc 1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct 1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg 1381 aattcgccag cacagtggtc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc 1441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 1501 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 1561 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 1621 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 1681 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 1741 aaaaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 1801 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 1861 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 1921 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 1981 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 2041 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 2101 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 2161 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 2221 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 2281 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 2341 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc 2401 catgcatcga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac 2461 cccacctgta ggtttggcaa gctagacgga tcgggagatc ttcgcaaaac gctgggattc 2521 ccggattaca ggcgggcgca ccacaccagg agcaaacact tccggtttta aaaattcagt 2581 ttgtgattgg ctgtcattca gtattatgct aattaagcat gcccggtttt aaacctctta 2641 aaacaacttt taaaattacc tttccaccta aaacgttaaa atttgtcaag tgataatatt 2701 cgaaaagctg ttattgccaa actattttcc tatttgtttc ctaatggcat cggaactagc 2761 gaaagtttct cgccatcagt taaaagtttg cggcagatgt agacctagca gaggtgtgcg 2821 aggagaagct tgggttgcgg agggtgggcc tgggaggggt ggtggccatt ttttgtctaa 2881 ccctaactga gaagggcgta ggcgccgtgc ttttgctccc cgcgcgctgt ttttctcgct 2941 gactttcagc gggcggaaaa gcctcggcct gccgccttcc accgttcatt ctagagcaaa 3001 caaaaaatgt cagctgctgg cccgttcgcc cctcccgggg acctgcggcg ggtcgcctgc 3061 ccagcccccg aaccccgcct ggaggccgcg gtcggcccgg ggcttctccg gaggcaccca 3121 ctgccaccgc gaagagttgg gctctgtcag ccgcgggtct ctcgggggcg agggcgaggt 3181 tcaggccttt caggccgcag gaagaggaac ggagcgagtc cccgcgcgcg gcgcgattcc 3241 ctgagctgtg ggacgtgcac ccaggactcg gctcacacat gcagttcgct ttcctgttgg 3301 tggggggaac gccgatcgtg cgcatccgtc acccctcgcc ggcagtgggg gcttgtgaac 3361 ccccaaacct gactgactgg gccagtgtgc tgcaaattgg caggagacgt gaaggcacct 3421 ccaaagtcgg ccaaaatgaa tgggcagtga gccggggttg cctggagccg ttcctgcgtg 3481 ggttctcccg tcttccgctt tttgttgcct tttatggttg tattacaact tagttcctgc 3541 tctgcagatt ttgttgaggt ttttgcttct cccaaggtag atctcgacca gtcccctcaa 3601 cggggtgtgg gagaacagtc attttttttt gagagatcat ttaacattta atgaatattt 3661 aattagaaga tctaaatgaa cattggaaat tgtgttcctt taatggtcat cggtttatgc 3721 cagaggttag aagtttcttt tttgaaaaat tagaccttgg cgatgacctt gagcagtagg 3781 atataacccc cacaagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag 3841 agcggccgcc accgcggtgg agctccaatt cgccctatag tgagtcgtat tacaattcac 3901 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 3961 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 4021 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 4081 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4141 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4201 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4261 aaaaacttga ttagggtgat ggttcactag cttaagtaac gccattttgc aaggcatgga 4321 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg 4381 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa 4441 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 4501 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga 4561 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac 4621 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa 4681 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac 4741 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg 4801 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta 4861 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa 4921 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 4981 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5041 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5101 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5161 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5221 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5281 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5341 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 5401 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 5461 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 5521 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 5581 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 5641 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 5701 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5761 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5821 aaaaggatct tcacctagat ccttttgcgg ccgcaaatca atctaaagta tatatgagta 5881 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 5941 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 6001 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 6061 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 6121 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 6181 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 6241 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 6301 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 6361 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 6421 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 6481 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 6541 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 6601 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 6661 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 6721 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 6781 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 6841 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcag c //