LOCUS Exported 5768 bp ds-DNA circular SYN 04-AUG-2021 DEFINITION mouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cells. ACCESSION . VERSION . KEYWORDS pcDNA3.1(+)-C-6His-mRplp1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5768) TITLE Bacterial expression plasmids for production of mEsrra protein JOURNAL Unpublished REFERENCE 2 (bases 1 to 5768) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 4, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5768 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1088..1105 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(1125..1142) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1131..1355 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1401..1829 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1488..1507) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1698..1719 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(1838..1858) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1843..2172 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2023..2158 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2085..2104 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2239..3033 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2293..2312) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2903..2922 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3207..3328 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3244..3263) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3298..3317 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3377..3393) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3377..3393) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3390..3412) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3401..3417 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3425..3455) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3470..3491 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3608..3625) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3779..4367) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3859..3878) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4538..5398) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5161..5180 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5399..5503) /gene="bla" /label=AmpR promoter primer_bind complement(5578..5597) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc gaattctgca gatatccagc 721 acagtggcgg ccgctcgaga tggcttccgt ctccgagctc gcttgcatct actccgccct 781 catcctgcac gacgacgagg tgacggtcac ggaggataag atcaatgccc tcattaaagc 841 agctggtgtc agcgtcgaac ctttctggcc tggcttgttt gccaaggctc tggccaatgt 901 caacattggg agcctcatct gcaatgtagg ggctggtggg cccgctccag cagctggagc 961 tgcgcctgct ggtggtgctg ctccatccac tgccgccgcc ccagctgagg agaagaaagt 1021 ggaggcaaag aaggaagagt ccgaggagtc tgaagatgac atgggcttcg gtctttttga 1081 cgggccccat catcaccatc accattgata aacccgctga tcagcctcga ctgtgccttc 1141 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 1201 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 1261 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 1321 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 1381 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 1441 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 1501 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 1561 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 1621 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 1681 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 1741 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 1801 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 1861 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 1921 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 1981 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 2041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 2101 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 2161 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 2221 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 2281 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 2341 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 2401 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 2461 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 2521 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 2581 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 2641 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 2701 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 2761 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 2821 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 2881 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 2941 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 3001 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 3061 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 3121 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 3181 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 3241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 3301 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 3361 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 3421 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 3481 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 3541 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 3601 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 3661 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 3721 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 3781 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 3841 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 3901 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 3961 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 4021 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 4081 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 4141 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 4201 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 4261 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 4321 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 4381 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 4441 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 4501 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 4561 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 4621 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 4681 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 4741 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 4801 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 4861 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 4921 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 4981 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 5041 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 5101 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 5161 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 5221 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 5281 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 5341 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 5401 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 5461 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 5521 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac 5581 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt 5641 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 5701 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata 5761 tacgcgtt //