LOCUS Exported 6716 bp ds-DNA circular SYN 16-AUG-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS -1748 human cyclin D1 promoter pGL3Basic SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6716) AUTHORS Tetsu O, McCormick F TITLE Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. JOURNAL Nature. 1999 Apr 1;398(6726):422-6. PUBMED 10201372 REFERENCE 2 (bases 1 to 6716) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6716 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 1931..1940 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1937..3589 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(2003..2021) /label=LucNrev /note="Firefly luciferase, reverse primer" polyA_signal 3630..3751 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3641..3660) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3695..3714 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(3999..4016) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4170..4758) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4250..4269) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4929..5789) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5552..5571 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5790..5894) /gene="bla" /label=AmpR promoter rep_origin 5921..6376 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6008..6027) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6218..6239 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 6507..6555 /note="synthetic polyadenylation signal" misc_feature 6569..6660 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 6609..6628 /label=RVprimer3 /note="pGL3 vector, forward primer" ORIGIN 1 cccctgggcc gcccttgtgc gcgggctgat gctctgaggc ttggctatgc gggggccaac 61 gcgattgtgg gtgctcgggg agtggggggg ggcacgaccg taggtgctcc ctgctggggc 121 aacccatcgc tccccatgcg gaatccgggg gtaattaccc cccaggaccc ggaatattag 181 taatcctaat tcccggcggg ggagggggcg cgggaggaat tcaccctgaa aggtgggggt 241 ggggggggtc gcatcttgct gtgagcaccc tggcgaaggg gagagggctt tttctatcag 301 ttttctttga gcttttactg ttaagagggt acggtggttt gatgacactg aactatattc 361 aaaaggaagt aaatgaacag ttttcttaat ttggggcagg tactgtaaaa ataaaaacaa 421 aagttaagac agtaaaatgt ccttttattt tttaatgcac caaagagaca gaacctgtaa 481 ttttaaaaac tgtgtatttt aatttacatc tgcttaagtt tgcgataata ttggggaccc 541 tctcatgtaa ccacgaacac ctatcgattt tgctaaaaat cagatcagta cactcgtttg 601 tttaattgat aattgttctg aattatgccg gctcctgcca gccccctcac gctcacgaat 661 tcagtcccag ggcaaattct aaaggtgaag ggacgtctac acccccaaca aaaccaatta 721 ggaaccttcg gtggtcttgt cccaggcaga ggggactaat atttccagca atttaatttc 781 ttttttaatt aaaaaaaatg agtcagaatg gagatcactg tttctcagct ttccattcag 841 aggtgtgttt ctcccggtta aattgccggc acgggaaggg agggggtgca gttggggacc 901 cccgcaagga ccgactggtc aaggtaggaa ggcagcccga agagtctcca ggctagaagg 961 acaagatgaa ggaaatgctg gccaccatct tgggctgctg ctggaatttt cgggcattta 1021 ttttatttta ttttttgagc gagcgcatgc taagctgaaa tccctttaac ttttagggtt 1081 acccccttgg gcatttgcaa cgacgcccct gtgcgccgga atgaaacttg cacaggggtt 1141 gtgtgcccgg tcctccccgt ccttgcatgc taaattagtt cttgcaattt acacgtgtta 1201 atgaaaatga aagaagatgc agtcgctgag attctttggc cgtctgtccg cccgtgggtg 1261 ccctcgtggc gttcttggaa atgcgcccat tctgccggct tggatatggg gtgtcgccgc 1321 gccccagtca ccccttctcg tggtctcccc aggctgcgtg tggcctgccg gccttcctag 1381 ttgtccccta ctgcagagcc acctccacct caccccctaa atcccggggg acccactcga 1441 ggcggacggg gccccctgca cccctcttcc ctggcgggga gaaaggctgc agcggggcga 1501 tttgcatttc tatgaaaacc ggactacagg ggcaactccg ccgcagggca ggcgcggcgc 1561 ctcagggatg gcttttgggc tctgcccctc gctgctcccg gcgtttggcg cccgcgcccc 1621 ctccccctgc gcccgccccc gcccccctcc cgctcccatt ctctgccggg ctttgatctt 1681 tgcttaacaa cagtaacgtc acacggacta caggggagtt ttgttgaagt tgcaaagtcc 1741 tggagcctcc agagggctgt cggcgcagta gcagcgagca gcagagtccg cacgctccgg 1801 cgaggggcag aagagcgcga gggagcgcgg ggcagcagaa gcgagagccg agcgcggacc 1861 cagccaggac ccacagccct ccccaggggt tcgaaatcga taagcttggc attccggtac 1921 tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta 1981 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct 2041 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga 2101 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa 2161 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc 2221 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct 2281 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa 2341 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa 2401 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg 2461 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat 2521 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc 2581 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac 2641 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata 2701 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag 2761 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt 2821 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg 2881 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg 2941 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg 3001 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga 3061 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc 3121 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga 3181 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt 3241 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga 3301 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga 3361 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga 3421 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg 3481 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat 3541 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg 3601 gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac 3661 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 3721 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 3781 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 3841 taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 3901 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 3961 aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc 4021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 4081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 4141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 4201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 4261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 4321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 4381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 4441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 4501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 4561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 4621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 4681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 4741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 4801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 4861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 4921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 4981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 5041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 5101 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 5161 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 5221 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 5281 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 5341 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 5401 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 5461 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 5521 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 5581 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 5641 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 5701 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 5761 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 5821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 5881 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 5941 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 6001 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 6061 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 6121 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 6181 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 6241 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 6301 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 6361 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 6421 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag 6481 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg 6541 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac 6601 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct 6661 atcgataggt accgagctct tacgcgtgct agcccgggct cgagatcccc gggtac //