LOCUS       Exported                7409 bp ds-DNA     circular SYN 16-AUG-2021
DEFINITION  AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV2/1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7409)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     111..133
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     125..142
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     126..142
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     152..171
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        152..170
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     195..211
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     196..212
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4201..4212
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4515..4531)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4515..4531)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4747..4767)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4747..4765)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4786..4802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4786..4802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4799..4821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4810..4826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4834..4864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5017..5034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5188..5776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5268..5287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5947..6807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6570..6589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6808..6912)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6938..7393)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7075..7096)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7287..7306
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
       61 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      121 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
      181 taccgggccc cccctcgagg tcgacggtat cgggggagct cgcagggtct ccattttgaa
      241 gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg attaaggtcc
      301 ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac tgggtggccg
      361 agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt gagcaggcac
      421 ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgc cgtgtgagta
      481 aggccccgga ggctcttttc tttgtgcaat ttgagaaggg agagagctac ttccacatgc
      541 acgtgctcgt ggaaaccacc ggggtgaaat ccatggtttt gggacgtttc ctgagtcaga
      601 ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg ccaaactggt
      661 tcgcggtcac aaagaccaga aatggcgccg gaggcgggaa caaggtggtg gatgagtgct
      721 acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg tggactaata
      781 tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg gtggcgcagc
      841 atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat cccaattctg
      901 atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc gggtggctcg
      961 tggacaaggg gattacctcg gagaagcagt ggatccagga ggaccaggcc tcatacatct
     1021 ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa
     1081 agattatgag cctgactaaa accgcccccg actacctggt gggccagcag cccgtggagg
     1141 acatttccag caatcggatt tataaaattt tggaactaaa cgggtacgat ccccaatatg
     1201 cggcttccgt ctttctggga tgggccacga aaaagttcgg caagaggaac accatctggc
     1261 tgtttgggcc tgcaactacc gggaagacca acatcgcgga ggccatagcc cacactgtgc
     1321 ccttctacgg gtgcgtaaac tggaccaatg agaactttcc cttcaacgac tgtgtcgaca
     1381 agatggtgat ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag
     1441 ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg gcccagatag
     1501 acccgactcc cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt gacgggaact
     1561 caacgacctt cgaacaccag cagccgttgc aagaccggat gttcaaattt gaactcaccc
     1621 gccgtctgga tcatgacttt gggaaggtca ccaagcagga agtcaaagac tttttccggt
     1681 gggcaaagga tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag ggtggagcca
     1741 agaaaagacc cgcccccagt gacgcagata taagtgagcc caaacgggtg cgcgagtcag
     1801 ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa ctacgcagac aggtaccaaa
     1861 acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga
     1921 gaatgaatca gaattcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct
     1981 ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct
     2041 acattcatca tatcatggga aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg
     2101 tggatttgga tgactgcatc tttgaacaat aaatgattta aatcaggtat ggctgccgat
     2161 ggttatcttc cagattggct cgaggacaac ctctctgagg gcattcgcga gtggtgggac
     2221 ttgaaacctg gagccccgaa gcccaaagcc aaccagcaaa agcaggacga cggccggggt
     2281 ctggtgcttc ctggctacaa gtacctcgga cccttcaacg gactcgacaa gggggagccc
     2341 gtcaacgcgg cggacgcagc ggccctcgag cacgacaagg cctacgacca gcagctcaaa
     2401 gcgggtgaca atccgtacct gcggtataac cacgccgacg ccgagtttca ggagcgtctg
     2461 caagaagata cgtcttttgg gggcaacctc gggcgagcag tcttccaggc caagaagcgg
     2521 gttctcgaac ctctcggtct ggttgaggaa ggcgctaaga cggctcctgg aaagaaacgt
     2581 ccggtagagc agtcgccaca agagccagac tcctcctcgg gcatcggcaa gacaggccag
     2641 cagcccgcta aaaagagact caattttggt cagactggcg actcagagtc agtccccgat
     2701 ccacaacctc tcggagaacc tccagcaacc cccgctgctg tgggacctac tacaatggct
     2761 tcaggcggtg gcgcaccaat ggcagacaat aacgaaggcg ccgacggagt gggtaatgcc
     2821 tcaggaaatt ggcattgcga ttccacatgg ctgggcgaca gagtcatcac caccagcacc
     2881 cgcacctggg ccttgcccac ctacaataac cacctctaca agcaaatctc cagtgcttca
     2941 acgggggcca gcaacgacaa ccactacttc ggctacagca ccccctgggg gtattttgat
     3001 ttcaacagat tccactgcca cttttcacca cgtgactggc agcgactcat caacaacaat
     3061 tggggattcc ggcccaagag actcaacttc aaactcttca acatccaagt caaggaggtc
     3121 acgacgaatg atggcgtcac aaccatcgct aataacctta ccagcacggt tcaagtcttc
     3181 tcggactcgg agtaccagct tccgtacgtc ctcggctctg cgcaccaggg ctgcctccct
     3241 ccgttcccgg cggacgtgtt catgattccg caatacggct acctgacgct caacaatggc
     3301 agccaagccg tgggacgttc atccttttac tgcctggaat atttcccttc tcagatgctg
     3361 agaacgggca acaactttac cttcagctac acctttgagg aagtgccttt ccacagcagc
     3421 tacgcgcaca gccagagcct ggaccggctg atgaatcctc tcatcgacca atacctgtat
     3481 tacctgaaca gaactcaaaa tcagtccgga agtgcccaaa acaaggactt gctgtttagc
     3541 cgtgggtctc cagctggcat gtctgttcag cccaaaaact ggctacctgg accctgttat
     3601 cggcagcagc gcgtttctaa aacaaaaaca gacaacaaca acagcaattt tacctggact
     3661 ggtgcttcaa aatataacct caatgggcgt gaatccatca tcaaccctgg cactgctatg
     3721 gcctcacaca aagacgacga agacaagttc tttcccatga gcggtgtcat gatttttgga
     3781 aaagagagcg ccggagcttc aaacactgca ttggacaatg tcatgattac agacgaagag
     3841 gaaattaaag ccactaaccc tgtggccacc gaaagatttg ggaccgtggc agtcaatttc
     3901 cagagcagca gcacagaccc tgcgaccgga gatgtgcatg ctatgggagc attacctggc
     3961 atggtgtggc aagatagaga cgtgtacctg cagggtccca tttgggccaa aattcctcac
     4021 acagatggac actttcaccc gtctcctctt atgggcggct ttggactcaa gaacccgcct
     4081 cctcagatcc tcatcaaaaa cacgcctgtt cctgcgaatc ctccggcgga gttttcagct
     4141 acaaagtttg cttcattcat cacccaatac tccacaggac aagtgagtgt ggaaattgaa
     4201 tgggagctgc agaaagaaaa cagcaagcgc tggaatcccg aagtgcagta cacatccaat
     4261 tatgcaaaat ctgccaacgt tgattttact gtggacaaca atggacttta tactgagcct
     4321 cgccccattg gcacccgtta ccttacccgt cccctgtaat tacgtgttaa tcaataaacc
     4381 ggttgattcg tttcagttga actttggtct cctgtccttc ttatcttatc ggttaccatg
     4441 gttatagctt acacattaac tgcttggttg cgcttcgcga taaaagactt acgtcatcgg
     4501 gttaccccta gggggatcca ctagttctag aggtcctgta ttagaggtca cgtgagtgtt
     4561 ttgcgacatt ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca
     4621 gggtctccat tttgaagcgg gaggtttgaa cgcgcagccg ccaagccgaa ttctgcagat
     4681 atccatcaca ctggcggccg ctcgactaga gcggccgcca ccgcggtgga gctccagctt
     4741 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     4801 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4861 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4921 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4981 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5041 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5101 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5161 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5221 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5281 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5461 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5521 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5581 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5641 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5701 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5881 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5941 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6001 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6061 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6121 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6181 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6241 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6301 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6361 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6421 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6481 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6541 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6601 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6661 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6721 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6781 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6841 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6901 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     6961 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     7021 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     7081 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     7141 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     7201 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     7261 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     7321 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     7381 ctacagggcg cgtcccattc gccattcag
//
