LOCUS Exported 9326 bp ds-DNA circular SYN 16-AUG-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBS-CMV-gagpol SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9326) TITLE MLV retroviral system JOURNAL Unpublished REFERENCE 2 (bases 1 to 9326) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9326 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 96..475 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 476..679 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 630..650 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 676..700 /label=LNCX /note="Human CMV promoter, forward primer" CDS 837..1253 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1183..1205 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1221..1237 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 5613..5987 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 5925..5941 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" primer_bind complement(6122..6139) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6128..6352 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6426..6445) /label=T7 /note="T7 promoter, forward primer" promoter complement(6427..6445) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6452..6469) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6452..6468) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6461..6483) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6610..7065 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6697..6716) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6907..6928 /label=F1ori-F /note="F1 origin, forward primer" promoter 7091..7195 /gene="bla" /label=AmpR promoter CDS 7196..8056 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7414..7433) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8227..8815 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8716..8735 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8969..8986 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9103..9124 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9139..9169 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9177..9193 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9182..9204 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9201..9217 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9201..9217 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 9238..9256 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ataaatcaat attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat 61 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta 121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 661 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg 721 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccaagcttgg 781 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatatgggcc 841 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg 901 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat 961 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc 1021 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca 1081 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc 1141 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt 1201 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccaaaccta 1261 aacctcaagt tctttctgac agtggggggc cgctcatcga cctacttaca gaagaccccc 1321 cgccttatag ggacccaaga ccaccccctt ccgacaggga cggaaatggt ggagaagcga 1381 cccctgcggg agaggcaccg gacccctccc caatggcatc tcgcctacgt gggagacggg 1441 agccccctgt ggccgactcc actacctcgc aggcattccc cctccgcgca ggaggaaacg 1501 gacagcttca atactggccg ttctcctctt ctgaccttta caactggaaa aataataacc 1561 cttctttttc tgaagatcca ggtaaactga cagctctgat cgagtctgtc ctcatcaccc 1621 atcagcccac ctgggacgac tgtcagcagc tgttggggac tctgctgacc ggagaagaaa 1681 aacaacgggt gctcttagag gctagaaagg cggtgcgggg cgatgatggg cgccccactc 1741 aactgcccaa tgaagtcgat gccgcttttc ccctcgagcg cccagactgg gattacacca 1801 cccaggcagg taggaaccac ctagtccact atcgccagtt gctcctagcg ggtctccaaa 1861 acgcgggcag aagccccacc aatttggcca aggtaaaagg aataacacaa gggcccaatg 1921 agtctccctc ggccttccta gagagactta aggaagccta tcgcaggtac actccttatg 1981 accctgagga cccagggcaa gaaactaatg tgtctatgtc tttcatttgg cagtctgccc 2041 cagacattgg gagaaagtta gagaggttag aagatttaaa aaacaagacg cttggagatt 2101 tggttagaga ggcagaaaag atctttaata aacgagaaac cccggaagaa agagaggaac 2161 gtatcaggag agaaacagag gaaaaagaag aacgccgtag gacagaggat gagcagaaag 2221 agaaagaaag agatcgtagg agacatagag agatgagcaa gctattggcc actgtcgtta 2281 gtggacagaa acaggataga cagggaggag aacgaaggag gtcccaactc gatcgcgacc 2341 agtgtgccta ctgcaaagaa aaggggcact gggctaaaga ttgtcccaag aaaccacgag 2401 gacctcgggg accaagaccc cagacctccc tcctgaccct agatgactag ggaggtcagg 2461 gtcaggagcc cccccctgaa cccaggataa ccctcaaagt cggggggcaa cccgtcacct 2521 tcctggtaga tactggggcc caacactccg tgctgaccca aaatcctgga cccctaagtg 2581 ataagtctgc ctgggtccaa ggggctactg gaggaaagcg gtatcgctgg accacggatc 2641 gcaaagtaca tctagctacc ggtaaggtca cccactcttt cctccatgta ccagactgtc 2701 cctatcctct gttaggaaga gatttgctga ctaaactaaa agcccaaatc cactttgagg 2761 gatcaggagc tcaggttatg ggaccaatgg ggcagcccct gcaagtgttg accctaaata 2821 tagaagatga gtatcggcta catgagacct caaaagagcc agatgtttct ctagggtcca 2881 catggctgtc tgattttcct caggcctggg cggaaaccgg gggcatggga ctggcagttc 2941 gccaagctcc tctgatcata cctctgaaag caacctctac ccccgtgtcc ataaaacaat 3001 accccatgtc acaagaagcc agactgggga tcaagcccca catacagaga ctgttggacc 3061 agggaatact ggtaccctgc cagtccccct ggaacacgcc cctgctaccc gttaagaaac 3121 cagggactaa tgattatagg cctgtccagg atctgagaga agtcaacaag cgggtggaag 3181 acatccaccc caccgtgccc aacccttaca acctcttgag cgggctccca ccgtcccacc 3241 agtggtacac tgtgcttgat ttaaaggatg cctttttctg cctgagactc caccccacca 3301 gtcagcctct cttcgccttt gagtggagag atccagagat gggaatctca ggacaattga 3361 cctggaccag actcccacag ggtttcaaaa acagtcccac cctgtttgat gaggcactgc 3421 acagagacct agcagacttc cggatccagc acccagactt gatcctgcta cagtacgtgg 3481 atgacttact gctggccgcc acttctgagc tagactgcca acaaggtact cgggccctgt 3541 tacaaaccct agggaacctc gggtatcggg cctcggccaa gaaagcccaa atttgccaga 3601 aacaggtcaa gtatctgggg tatcttctaa aagagggtca gagatggctg actgaggcca 3661 gaaaagagac tgtgatgggg cagcctactc cgaagacccc tcgacaacta agggagttcc 3721 tagggacggc aggcttctgt cgcctctgga tccctgggtt tgcagaaatg gcagccccct 3781 tgtaccctct caccaaaacg gggactctgt ttaattgggg cccagaccaa caaaaggcct 3841 atcaagaaat caagcaagct cttctaactg ccccagccct ggggttgcca gatttgacta 3901 agccctttga actctttgtc gacgagaagc agggctacgc caaaggtgtc ctaacgcaaa 3961 aactgggacc ttggcgtcgg ccggtggcct acctgtccaa aaagctagac ccagtagcag 4021 ctgggtggcc cccttgccta cggatggtag cagccattgc cgtactgaca aaggatgcag 4081 gcaagctaac catgggacag ccactagtca ttctggcccc ccatgcagta gaggcactag 4141 tcaaacaacc ccccgaccgc tggctttcca acgcccggat gactcactat caggccttgc 4201 ttttggacac ggaccgggtc cagttcggac cggtggtagc cctgaacccg gctacgctgc 4261 tcccactgcc tgaggaaggg ctgcaacaca actgccttga tatcctggcc gaagcccacg 4321 gaacccgacc cgacctaacg gaccagccgc tcccagacgc cgaccacacc tggtacacgg 4381 atggaagcag tctcttacaa gagggacagc gtaaggcggg agctgcggtg accaccgaga 4441 ccgaggtaat ctgggctaaa gccctgccag ccgggacatc cgctcagcgg gctgaactga 4501 tagcactcac ccaggcccta aagatggcag aaggtaagaa gctaaatgtt tatactgata 4561 gccgttatgc ttttgctact gcccatatcc atggagaaat atacagaagg cgtgggttgc 4621 tcacatcaga aggcaaagag atcaaaaata aagacgagat cttggcccta ctaaaagccc 4681 tctttctgcc caaaagactt agcataatcc attgtccagg acatcaaaag ggacacagcg 4741 ccgaggctag aggcaaccgg atggctgacc aagcggcccg aaaggcagcc atcacagaga 4801 ctccagacac ctctaccctc ctcatagaaa attcatcacc ctacacctca gaacattttc 4861 attacacagt gactgatata aaggacctaa ccaagttggg ggccatttat gataaaacaa 4921 agaagtattg ggtctaccaa ggaaaacctg tgatgcctga ccagtttact tttgaattat 4981 tagactttct tcatcagctg actcacctca gcttctcaaa aatgaaggct ctcctagaga 5041 gaagccacag tccctactac atgctgaacc gggatcgaac actcaaaaat atcactgaga 5101 cctgcaaagc ttgtgcacaa gtcaacgcca gcaagtctgc cgttaaacag ggaactaggg 5161 tccgcgggca tcggcccggc actcattggg agatcgattt caccgagata aagcccggat 5221 tgtatggcta taaatatctt ctagttttta tagatacctt ttctggctgg atagaagcct 5281 tcccaaccaa gaaagaaacc gccaaggtcg taaccaagaa gctactagag gagatcttcc 5341 ccaggttcgg catgcctcag gtattgggaa ctgacaatgg gcctgccttc gtctccaagg 5401 tgagtcagac agtggccgat ctgttgggga ttgattggaa attacattgt gcatacagac 5461 cccaaagctc aggccaggta gaaagaatga atagaaccat caaggagact ttaactaaat 5521 taacgcttgc aactggctct agagactggg tgctcctact ccccttagcc ctgtaccgag 5581 cccgcaacac gccgggcccc catggcctca ccccatatga gatcttatat ggggcacccc 5641 cgccccttgt aaacttccct gaccctgaca tgacaagagt tactaacagc ccctctctcc 5701 aagctcactt acaggctctc tacttagtcc agcacgaagt ctggagacct ctggcggcag 5761 cctaccaaga acaactggac cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag 5821 tgtgggtccg ccgacaccag actaagaacc tagaacctcg ctggaaagga ccttacacag 5881 tcctgctgac cacccccacc gccctcaaag tagacggcat cgcagcttgg atacacgccg 5941 cccacgtgaa ggctgccgac cccgggggtg gaccatcctc tagactgaca tggcgcgttc 6001 aacgctctca aaacccctta aaaataaggt tgacgcgtca acccgcgagg ccccctaatc 6061 cccttaattc ttctgatgct cagaggggtc agtatcgaat tcctgcagcc ccgctgatca 6121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 6181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 6241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 6301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 6361 gcggaaagaa ccagctgggg ctcgatcctc tagagcggcc gccaccgcgg tggagctcca 6421 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact 6481 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 6541 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 6601 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 6661 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 6721 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 6781 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 6841 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 6901 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 6961 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 7021 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 7081 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 7141 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 7201 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 7261 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 7321 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 7381 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 7441 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 7501 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 7561 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 7621 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 7681 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 7741 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 7801 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 7861 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 7921 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 7981 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 8041 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 8101 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 8161 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 8221 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 8281 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 8341 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 8401 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 8461 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 8521 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 8581 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 8641 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 8701 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 8761 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 8821 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 8881 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 8941 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 9001 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 9061 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 9121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 9181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat 9241 taaccctcac taaagggaac aaaagctggt accgggcccc ccctcgagat ccggccatta 9301 gccatattat tcattggtta tatagc //