LOCUS       Exported                7072 bp ds-DNA     circular SYN 16-AUG-2021
DEFINITION  mammalian expression of human Beta-catenin GFP.
ACCESSION   .
VERSION     .
KEYWORDS    Human Beta-catenin GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Han SP, Gambin Y, Gomez GA, Verma S, Giles N, Michael M, Wu SK, Guo 
            Z, Johnston W, Sierecki E, Parton RG, Alexandrov K, Yap AS
  TITLE     Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula 
            adherens.
  JOURNAL   J Biol Chem. 2014 Mar 14;289(11):7764-75. doi: 
            10.1074/jbc.M113.544478. Epub 2014 Jan 27.
  PUBMED    24469447
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      658..667
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             664..3006
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLASPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             664..3006
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=-beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLASPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     regulatory      3019..3028
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3025..3744
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3025..3741
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3070..3091)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3331..3350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3678..3699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3867..3988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3904..3923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3958..3977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3995..4450)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4132..4153)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4344..4363
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4477..4581
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4583..4940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4606..4626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4791..4926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4853..4872
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4975..5769
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5029..5048)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5639..5658
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5957..5976)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6001..6048
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6377..6965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6866..6885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcc
      661 accatggcta ctcaagctga tttgatggag ttggacatgg ccatggaacc agacagaaaa
      721 gcggctgtta gtcactggca gcaacagtct tacctggact ctggaatcca ttctggtgcc
      781 actaccacag ctccttctct gagtggtaaa ggcaatcctg aggaagagga tgtggatacc
      841 tcccaagtcc tgtatgagtg ggaacaggga ttttctcagt ccttcactca agaacaagta
      901 gctgatattg atggacagta tgcaatgact cgagctcaga gggtacgagc tgctatgttc
      961 cctgagacat tagatgaggg catgcagatc ccatctacac agtttgatgc tgctcatccc
     1021 actaatgtcc agcgtttggc tgaaccatca cagatgctga aacatgcagt tgtaaacttg
     1081 attaactatc aagatgatgc agaacttgcc acacgtgcaa tccctgaact gacaaaactg
     1141 ctaaatgacg aggaccaggt ggtggttaat aaggctgcag ttatggtcca tcagctttct
     1201 aaaaaggaag cttccagaca cgctatcatg cgttctcctc agatggtgtc tgctattgta
     1261 cgtaccatgc agaatacaaa tgatgtagaa acagctcgtt gtaccgctgg gaccttgcat
     1321 aacctttccc atcatcgtga gggcttactg gccatcttta agtctggagg cattcctgcc
     1381 ctggtgaaaa tgcttgcttc accagtggat tctgtgttgt tttatgccat tacaactctc
     1441 cacaaccttt tattacatca agaaggagct aaaatggcag tgcgtttagc tggtgggctg
     1501 cagaaaatgg ttgccttgct caacaaaaca aatgttaaat tcttggctat tacgacagac
     1561 tgccttcaaa ttttagctta tggcaaccaa gaaagcaagc tcatcatact ggctagtggt
     1621 ggaccccaag ctttagtaaa tataatgagg acctatactt acgaaaaact actgtggacc
     1681 acaagcagag tgctgaaggt gctatctgtc tgctctagta ataagccggc tattgtagaa
     1741 gctggtggaa tgcaagcttt aggacttcac ctgacagatc caagtcaacg tcttgttcag
     1801 aactgtcttt ggactctcag gaatctttca gatgctgcaa ctaaacagga agggatggaa
     1861 ggtctccttg ggactcttgt tcagcttctg ggttcagatg atataaatgt ggtcacctgt
     1921 gcagctggaa ttctttctaa cctcacttgc aataattata agaacaagat gatggtctgc
     1981 caagtgggtg gtatagaggc tcttgtgcgt actgtccttc gggctggtga cagggaagac
     2041 atcactgagc ctgccatctg tgctcttcgt catctgacca gccgacacca agaagcagag
     2101 atggcccaga atgcagttcg ccttcactat ggactaccag ttgtggttaa gctcttacac
     2161 ccaccatccc actggcctct gataaaggct actgttggat tgattcgaaa tcttgccctt
     2221 tgtcccgcaa atcatgcacc tttgcgtgag cagggtgcca ttccacgact agttcagttg
     2281 cttgttcgtg cacatcagga tacccagcgc cgtacgtcca tgggtgggac acagcagcaa
     2341 tttgtggagg gggtccgcat ggaagaaata gttgaaggtt gtaccggagc ccttcacatc
     2401 ctagctcggg atgttcacaa ccgaattgtt atcagaggac taaataccat tccattgttt
     2461 gtgcagctgc tttattctcc cattgaaaac atccaaagag tagctgcagg ggtcctctgt
     2521 gaacttgctc aggacaagga agctgcagaa gctattgaag ctgagggagc cacagctcct
     2581 ctgacagagt tacttcactc taggaatgaa ggtgtggcga catatgcagc tgctgttttg
     2641 ttccgaatgt ctgaggacaa gccacaagat tacaagaaac ggctttcagt tgagctgacc
     2701 agctctctct tcagaacaga gccaatggct tggaatgaga ctgctgatct tggacttgat
     2761 attggtgccc agggagaacc ccttggatat cgccaggatg atcctagcta tcgttctttt
     2821 cactctggtg gatatggcca ggatgccttg ggtatggacc ccatgatgga acatgagatg
     2881 ggtggccacc accctggtgc tgactatcca gttgatgggc tgccagatct ggggcatgcc
     2941 caggacctca tggatgggct gcctccaggt gacagcaatc agctggcctg gtttgatact
     3001 gacctggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3061 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3181 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3241 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3481 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3601 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3721 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     3781 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3841 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3961 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     4021 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4081 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4141 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4201 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4261 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4321 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4381 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4441 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4561 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4621 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4681 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4741 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4921 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4981 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5041 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5101 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     5161 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5221 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5281 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5341 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5401 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5461 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     5521 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5581 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5641 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5701 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5761 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5821 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5881 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5941 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     6001 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     6061 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     6121 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     6181 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     6241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gc
//
