LOCUS       Exported                5992 bp ds-DNA     circular SYN 16-AUG-2021
DEFINITION  Localization: Golgi Complex, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    mCherry-TGNP-N-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5992)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1938..1947
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1944..2654
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2089..2107)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2357..2376
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2777..2898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2814..2833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2868..2887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2905..3360)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3042..3063)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3254..3273
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3387..3491
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3493..3850
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3516..3536)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3701..3836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3763..3782
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3885..4679
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3939..3958)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4549..4568
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4867..4886)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4911..4958
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5287..5875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5776..5795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agctatgcca
      601 ccatgcggtt cgtggttgcc ttggtcctcc tgaacgtcgc agcggcggga gccgtgccgc
      661 tcttggccac cgaaagcgtc aagcaagaag aagctggagt acggccttct gcaggaaacg
      721 tctccaccca ccccagcttg agccaacggc ctggaggctc taccaagtcg catccggagc
      781 cgcagactcc aaaagacagc cctagcaagt cgagtgcgga ggcgcagacc ccagaagaca
      841 cccccaacaa gtcgggtgcg gaggcaaaga cccaaaaaga cagctccaac aagtcgggtg
      901 cggaggcaaa gacccaaaaa ggcagcacta gcaagtcggg ttcggaggcg cagaccacaa
      961 aagacagcac tagtaagtcg catccggagc tgcagactcc aaaagacagc actggcaaat
     1021 cgggtgcgga ggcgcagacc ccagaagaca gccccaacag gtcgggtgcg gaggcaaaga
     1081 cccaaaaaga cagccctagc aagtcaggtt cggaggcgca gaccacaaaa gatgtcccta
     1141 ataagtcggg tgcggacggc cagaccccaa aagacggctc cagcaagtcg ggtgcggagg
     1201 atcagacccc aaaagacgtc cctaacaagt cgggtgcgga gaagcagact ccaaaagacg
     1261 gctctaacaa gtccggtgca gaggagcagg gcccaataga cgggcccagc aagtcgggtg
     1321 cggaggagca gacctcaaaa gacagcccta acaaggtggt tccagagcag ccttcctgga
     1381 aagaccattc caagcccatc tccaaccctt ctgataacaa ggagctcccc aaggctgaca
     1441 caaaccagct tgctgacaaa gggaagcttt ctcctcatgc tttcaaaacc gaatctgggg
     1501 aggaaactga cctcatttct cccccgcagg aggaagttaa gtcttcagag cctactgagg
     1561 atgtggagcc caaagaggct gaagatgatg atacaggacc cgaggagggc tcaccgccca
     1621 aagaagagaa agaaaagatg tccggttctg cctccagtga gaaccgtgaa ggaacacttt
     1681 cggattccac gggtagcgag aaggatgacc tttatccgaa cggttctgga aatggcagcg
     1741 cggagagcag ccacttcttt gcatatctgg tgactgcagc cattcttgtg gctgtcctct
     1801 atatcgctca tcacaacaag cggaagatca ttgcttttgt cctggaagga aaaagatcta
     1861 aagtcacccg gcggccaaag gccagtgact accaacgttt ggaccagaag tccagctccg
     1921 gaggggatcc accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca
     1981 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     2041 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     2101 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     2161 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     2221 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     2281 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     2341 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     2401 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     2461 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     2521 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     2581 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     2641 agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     2701 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2761 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2881 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     2941 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3001 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3061 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3121 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3181 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3241 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3301 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3361 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3421 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3481 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     3541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3601 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3661 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3721 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3781 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3841 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     3901 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     3961 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4021 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     4081 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4141 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4201 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4261 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4321 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4381 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     4441 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4501 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4561 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4621 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4681 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4741 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4801 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     4861 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     4921 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     4981 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     5041 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     5101 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     5161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt ag
//
