LOCUS Exported 11178 bp ds-DNA circular SYN 18-AUG-2021 DEFINITION pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.. ACCESSION . VERSION . KEYWORDS pBHM2403 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11178) TITLE Short-homology genome engineering in Cryptococcus neoformans JOURNAL Unpublished REFERENCE 2 (bases 1 to 11178) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11178 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 166..185 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 419..436 /label=L4440 /note="L4440 vector, forward primer" protein_bind 553..574 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 589..619 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 627..643 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 632..654 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 651..667 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 651..667 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 688..706 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 734..750 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 735..751 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS complement(1630..1656) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS complement(1657..1677) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(1684..1704) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(1720..4104) /codon_start=1 /product="C-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)" /label=Cas9(C) /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD" primer_bind complement(7129..7148) /label=T7 /note="T7 promoter, forward primer" promoter complement(7130..7148) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7155..7172) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7155..7171) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7164..7186) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7312..7767 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7399..7418) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7609..7630 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(7898..8701) /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(8702..8917) /gene="S. cerevisiae URA3" /label=URA3 promoter primer_bind complement(8941..8960) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9060..9082 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(9120..9138) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 9179..9682 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" promoter 9719..9823 /gene="bla" /label=AmpR promoter CDS 9824..10684 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10042..10061) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10855..265 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 61 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 421 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 481 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 541 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 601 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 661 ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt 721 accgggcccc ccctcgaggt cgacggtatc gatcgttcaa tctcatagtc ttctaaagat 781 cccccgttcc caaacatgac attcaacatc ttcgcttcga cttccacctc gaccaacccc 841 ttttcttcca aatccactcg acgtgcgcca tccccctctt cctgccccat ccctcctccg 901 tttgccccgc caactcttcc caggttgatg aagggggtgc cctggcccga caagcgtcga 961 ggcgaggtaa gtgtgtggct gagattcaag gcattaacga ggacgtgtga gcaggtggaa 1021 gtgcggttgc ggtaggaagg acgaggtcag ggatgcgtag agacatcggt aattctgtac 1081 atctgtttct tgcttctttc cccgttcttt ccatgcaaca aactattatt acaacatgaa 1141 ctctcttagc atgacagtcc acagaaccga gatggtcagt gcatgtcgag attatttcgt 1201 ggtgatctcc aggaacgact gaaaaaagga gtgacggatt aaggaagacg tgaccgacta 1261 aaggaaagga aggatggaag gaacgatatc aatgcgatgg tggcgacctg agaatgagta 1321 aatagttgaa agcactaaaa taaagcagca acgaaggtta ttccaagtta tagctctcgt 1381 gcaaatttca acagtgatgg ccattttatt gttgcaagag acgtcgtcag ccgagaaata 1441 ttcgatttcg cggacaacta tcgggaatcc aataacggac aaacgacatg gaatgatata 1501 tcacggcaat gaagattttg aagcgcagag gtatacatca gcaaataaga cacaaaacgt 1561 aagaagcaca agtagcaaca gtcatccgat gcatattacg cgtaagaagg gtcactacat 1621 aagtaattag gcgtagtcgg ggacgtcgta ggggtagacc tttcgcttct tcttgggggg 1681 gtagaccttt cgcttcttct tggggggacc ggaaccaccg tcaccaccga gctgggagag 1741 gtcgattcgg gtctcgtaga gaccggtgat ggactggtgg atgagggtgg cgtcgaggac 1801 ctccttggtg gaggtgtatc gctttcggtc gatggtggtg tcgaagtact tgaaggcggc 1861 gggggcaccg aggttggtga gggtgaagag gtggatgatg ttctcggcct gctctcggat 1921 gggcttgtct cggtgcttgt tgtaggcgga gaggaccttg tcgaggttgg cgtcggcgag 1981 gatgactcgc ttggagaact cggagatctg ctcgatgatc tcgtcgaggt agtgcttgtg 2041 ctgctcgacg aagagctgct tctgctcgtt gtcctcgggg gaacccttga gcttctcgta 2101 gtgggaggcg aggtagagga agttgacgta cttggagggg agggcgagct cgttaccctt 2161 ctggagctca ccggcggagg cgagcattcg ctttcgaccg ttctcgagct cgaagaggga 2221 gtacttgggg agcttgatga tgaggtcctt cttgacctcc ttgtaaccct tggcctcgag 2281 gaagtcgatg gggttcttct cgaaggagga tcgctccatg atggtgatac cgaggagctc 2341 cttgacggac ttgagcttct tggacttacc cttctcgacc ttggcgacga cgaggacgga 2401 gtaggcgacg gtgggggagt cgaaaccacc gtacttcttg gggtcccagt ccttctttcg 2461 ggcgatgagc ttgtcggagt ttcgcttggg gaggatggac tccttggaga aaccaccggt 2521 ctggacctcg gtcttcttga cgatgttgac ctggggcatg gagaggacct ttcggacggt 2581 ggcgaagtct cgacccttgt cccagacgat ctcaccggtc tcaccgttgg tctcgatgag 2641 gggtcgcttt cggatctcac cgttggcgag ggtgatctcg gtcttgaaga agttcatgat 2701 gttggagtag aagaagtact tggcggtggc cttaccgatc tcctgctcgg acttggcgat 2761 catctttcgg acgtcgtaga ccttgtagtc accgtagacg aactcggact cgagcttggg 2821 gtacttcttg atgagggcgg taccgacgac ggcgttgagg taggcgtcgt gggcgtggtg 2881 gtagttgttg atctctcgga ccttgtagaa ctggaagtcc tttcggaagt cggagacgag 2941 cttggacttg agggtgatga ccttgacctc tcggatgagc ttgtcgttct cgtcgtactt 3001 ggtgttcatt cgggagtcga ggatctgggc gacgtgcttg gtgatctgtc gggtctcgac 3061 gagctgtcgc ttgatgaaac cggccttgtc gagctcggag agaccacctc gctcggcctt 3121 ggtgaggttg tcgaactttc gctgggtgat gagcttggcg ttgaggagct gtcgccagta 3181 gttcttcatc ttcttgacga cctcctcgga ggggacgttg tcggacttac ctcggttctt 3241 gtcggatcgg gtgaggacct tgttgtcgat ggagtcgtcc ttgaggaagg actgggggac 3301 gatgtggtcg acgtcgtagt cggagagtcg gttgatgtcg agctcctggt cgacgtacat 3361 gtctcgaccg ttctggaggt agtagaggta gagcttctcg ttctggagct gggtgttctc 3421 gacggggtgc tccttgagga tctgggaacc gagctccttg ataccctcct cgattcgctt 3481 cattcgctct cgggagttct tctgaccctt ctgggtggtc tggttctctc gggccatctc 3541 gatgacgatg ttctcgggct tgtgtcgacc catgaccttg acgagctcgt cgacgacctt 3601 gacggtctgg aggataccct tcttgatggc gggggaaccg gcgaggttgg cgatgtgctc 3661 gtggagggag tcaccctgac cggagacctg ggccttctgg atgtcctcct tgaaggtgag 3721 ggagtcgtcg tggatgagct gcatgaagtt tcggttggcg aaaccgtcgg acttgaggaa 3781 gtcgaggatg gtcttaccgg actgcttgtc tcggataccg ttgatgagct ttcgggagag 3841 tcgaccccaa ccggtgtatc gtcgtcgctt gagctgcttc atgaccttgt cgtcgaagag 3901 gtgggcgtag gtcttgagtc gctcctcgat catctctcgg tcctcgaaga gggtgagggt 3961 gaggacgatg tcctcgagga tgtcctcgtt ctcctcgttg tcgaggaagt ccttgtcctt 4021 gatgatcttg aggaggtcgt ggtaggtacc gagggaggcg ttgaatcggt cctcgacacc 4081 ggagatctcg acggagtcga aacactcgat cttcttgaag tagtcctcct tgagctgctt 4141 gacggtgacc tttcggttgg tcttgaagag gaggtcgacg atggccttct tctgctcacc 4201 ggagaggaag gcgggctttc gcataccctc ggtgacgtac ttgaccttgg tgagctcgtt 4261 gtagacggtg aagtactcgt agaggaggga gtgcttgggg aggaccttct cgttggggag 4321 gttcttgtcg aagttggtca ttcgctcgat gaaggactgg gcggaggcac ccttgtcgac 4381 gacctcctcg aagttccagg gggtgatggt ctcctcggac tttcgggtca tccaggcgaa 4441 tcgggagtta cctcgggcga ggggaccgac gtagtagggg attcggaagg tgaggatctt 4501 ctcgatcttc tctcggttgt ccttgaggaa ggggtagaag tcctcctgtc gtcggaggat 4561 ggcgtggagc tcaccgaggt ggatctggtg ggggatggaa ccgttgtcga aggttcgctg 4621 ctttcggagg aggtcctctc ggttgagctt gacgaggagc tcctcggtac cgtccatctt 4681 ctcgaggatg ggcttgatga acttgtagaa ctcctcctgg gaggcaccac cgtcgatgta 4741 accggcgtaa ccgttcttgg actggtcgaa gaagatctcc tgtcgggaga tgttagtgga 4801 gtaacatgtt gcgggaaacg gggttacata ccttgtactt ctcggggagc tgctgtcgga 4861 cgagggcctt gaggagggtg aggtcctggt ggtgctcgtc gtatcgcttg atcatggagg 4921 cggagagggg ggccttggtg atctcggtgt tgactcggag gatgtcggag aggaggatgg 4981 cgtcggagag gttcttggcg gcgaggaaga ggtcggcgta ctggtcaccg atctgggcga 5041 ggaggttgtc gaggtcgtcg tcgtaggtgt ccttggagag ctggagcttg gcgtcctcgg 5101 cgaggtcgaa gttggacttg aagttggggg tgagaccgag ggagagggcg atgaggttac 5161 cgaagagacc gttcttcttc tcaccgggga gctgggcgat gaggttctcg agtcgtcggg 5221 acttggagag tcgggcggag aggatggcct tggcgtcgac accggaggcg ttgatggggt 5281 tctcctcgaa gagctggttg taggtctgga cgagctggat gaagagcttg tcgacgtcgg 5341 agttgtcggg gttgaggtca ccctcgatga ggaagtgacc tcggaacttg atcatgtggg 5401 cgagggcgag gtagatgagt cggaggtcgg ccttgtcggt ggagtcgacg agcttctttc 5461 ggaggtggta gatggtgggg tacttctcgt ggtaggcgac ctcgtcgacg atgttaccga 5521 agatggggtg tcgctcgtgc ttcttgtcct cctcgacgag gaaggactcc tcgagtcggt 5581 ggaagaagga gtcgtcgacc ttggccatct cgttggagaa gatctcctgg aggtaacaga 5641 ttcggttctt tcgtcgggtg tatcgtcgtc gggcggttcg cttgagtcgg gtggcctcgg 5701 cggtctcacc ggagtcgaag aggagggcac cgatgaggtt cttcttgatg gagtgtcggt 5761 cggtgttacc gaggaccttg aacttcttgg aggggacctt gtactcgtcg gtgatgacgg 5821 cccaaccgac ggagttggta ccgatgtcga gaccgatgga gtacttcttg tccattttga 5881 agttttctgt ggagatcgtt agcatgtgcg aagaggcaat gataggacaa taaagtgtta 5941 gggcgttgtg agttggaagg gcatgtatcc gatgggttga aaattttcaa gagccaccaa 6001 atttttttct tcaccacaca caagcccatg ttgagctcag tagtggcaaa acagtactct 6061 gcagtcgacg caacagatga gggcaaaagc atggaagaca tcgccaaaaa caagacagaa 6121 tgatgtgggt ggtcgaccat gctcagctcc actctgcccc gacttcacct gcagaagcca 6181 ccagcgagaa ttgttttacg tgccctacga ctggcgcgga ttttcaaagg tgtcaaaaga 6241 ctttgggtac tcacctaggt gttttggtta aagaatgtag agataaagaa ggtggtggga 6301 gaaaggaatg tgcgagggat ttaattcttt ccggttgatg ggcagggcgg attctcggat 6361 taattcagaa aatcccacaa tttttttcga aacctccaga atgtgaaaag ctcactggaa 6421 atttcatccg tatcaaactc cgtttttcac cgagtgtggc acatgggact taggaggcaa 6481 gaaatgtgag gaggatggag cgaccgcaac aacggagaaa tttaaagtgg aggtcgttcc 6541 caggcacgtc ttcgcaccaa aaattcaacg ggtggcaaga ggcccctgcc gtctaaacat 6601 gtgggagtgg ctcatcctca caccgagcag gccaatgcat atgataagag tcgtccagaa 6661 gccgacgtgc atgaatggag cttcacaaaa tgcgaaatta gaagaagacg agatgcccat 6721 gatcttctgg gaagggggcg aggcaacttg cttggcgaat ggggctgaat attggggcgt 6781 tcgctaatgg ccatgacgct tgttcatggc agggtagaac acgacattat tgcttgtgcg 6841 tgggggcttc tggctgcttt ttgtacattg cgcgaagggt tgccatgatg agactgctgt 6901 attatgctct tattcttttg tttaaaggga gtgatacttg acataccgat gatagatagt 6961 taagaagcgc ccgcaggtgg gtcaaccaca cttgcgacca ttgttaatca ttcagaacgg 7021 tcgacaaggt cagtgtaaga tcattcgcta tggagcttgc atagtagcca caatcttgta 7081 ccgtatagca tagaatggcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag 7141 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 7201 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 7261 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct 7321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 7381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 7441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 7501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 7561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 7621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 7681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 7741 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg 7801 cggtatttca caccgcatag ggtaataact gatataatta aattgaagct ctaatttgtg 7861 agtttagtat acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc 7921 tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc 7981 cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat 8041 ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg 8101 tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc 8161 cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag 8221 tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg 8281 ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg 8341 cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga 8401 ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg 8461 ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat 8521 gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg 8581 tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata 8641 gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca 8701 tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc 8761 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct 8821 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa 8881 atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag gtggtatggt gcactctcag 8941 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 9001 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 9061 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 9121 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgga 9181 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt 9241 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag 9301 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa 9361 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc 9421 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac 9481 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat 9541 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt 9601 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat 9661 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg 9721 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 9781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 9841 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 9901 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 9961 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 10021 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 10081 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 10141 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 10201 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 10261 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 10321 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 10381 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 10441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 10501 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 10561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 10621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 10681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 10741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 10801 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 10861 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 10921 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 10981 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 11041 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 11101 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 11161 gcggtcgggc tgaacggg //