LOCUS Exported 10039 bp ds-DNA circular SYN 20-AUG-2021 DEFINITION CRISPaint frame selector +2. ACCESSION . VERSION . KEYWORDS pCAS9-mCherry-Frame +2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10039) AUTHORS Schmid-Burgk JL, Honing K, Ebert TS, Hornung V TITLE CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. JOURNAL Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. PUBMED 27465542 REFERENCE 2 (bases 1 to 10039) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10039 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 67..536 /note="long terminal repeat from Moloney murine leukemia virus" CDS 1005..1421 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1351..1373 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1389..1405 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" promoter 1432..1672 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 1432..1452 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 1603..1622 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 1701..1776 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 1817..2196 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2197..2400 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2351..2371 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 2461..2470 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2470..2493 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 2494..3198 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE GRHSTGGMDELYK" primer_bind complement(2636..2654) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2904..2923 /label=mCherry-F /note="mCherry, forward primer" CDS 3223..3276 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3277..7380 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 7393..7413 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" LTR 7592..8061 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(8191..8208) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8362..8950) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8442..8461) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9110..9970) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9733..9752 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9971..36) /gene="bla" /label=AmpR promoter ORIGIN 1 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 61 tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 121 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 181 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt 241 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 301 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 361 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 421 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 481 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg 541 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggc 601 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat 661 gcgcctgcgt cggtactagc tagttagcta actagctctg tatctggcgg acccgtggtg 721 gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg 781 gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat 841 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc 901 tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt 961 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca 1021 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt 1081 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta 1141 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca 1201 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg 1261 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc 1321 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc 1381 gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggccggatc cgagggccta 1441 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga 1501 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa 1561 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc 1621 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc 1681 gggccagtac ccaaaaagcg gttttagagc tagaaatagc aagttaaaat aaggctagtc 1741 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttcgaattc cgatgtacgg 1801 gccagatata cgcgttgaca ttgattattg tctagttatt aatagtaatc aattacgggg 1861 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 1921 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1981 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 2041 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2101 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2161 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 2221 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 2281 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 2341 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 2401 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaagct tccgcgtacg 2461 gccaccatgg attataagga tgacgacgat aaggtgagca agggcgagga ggataacatg 2521 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 2581 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 2641 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 2701 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 2761 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 2821 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc 2881 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 2941 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 3001 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 3061 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 3121 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 3181 atggacgagc tgtacaaggt cgctaccatc gagggatccg gagagggcag aggaagtctg 3241 ctaacatgcg gtgacgtcga ggagaatcct ggcccaatgg acaagaagta cagcatcggc 3301 ctggacatcg gcaccaacag cgtgggctgg gccgtgatca ccgacgagta caaggtgccc 3361 agcaagaagt tcaaggtgct gggcaacacc gaccgccaca gcatcaagaa gaacctgatc 3421 ggcgccctgc tgttcgacag cggcgagacc gccgaggcca cccgcctgaa gcgcaccgcc 3481 cgccgccgct acacccgccg caagaaccgc atctgctacc tgcaggagat cttcagcaac 3541 gagatggcca aggtggacga cagcttcttc caccgcctgg aggagagctt cctggtggag 3601 gaggacaaga agcacgagcg ccaccccatc ttcggcaaca tcgtggacga ggtggcctac 3661 cacgagaagt accccaccat ctaccacctg cgcaagaagc tggtggacag caccgacaag 3721 gccgacctgc gcctgatcta cctggccctg gcccacatga tcaagttccg cggccacttc 3781 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg 3841 gtgcagacct acaaccagct gttcgaggag aaccccatca acgccagcgg cgtggacgcc 3901 aaggccatcc tgagcgcccg cctgagcaag agccgccgcc tggagaacct gatcgcccag 3961 ctgcccggcg agaagaagaa cggcctgttc ggcaacctga tcgccctgag cctgggcctg 4021 acccccaact tcaagagcaa cttcgacctg gccgaggacg ccaagctgca gctgagcaag 4081 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac 4141 ctgttcctgg ccgccaagaa cctgagcgac gccatcctgc tgagcgacat cctgcgcgtg 4201 aacaccgaga tcaccaaggc ccccctgagc gccagcatga tcaagcgcta cgacgagcac 4261 caccaggacc tgaccctgct gaaggccctg gtgcgccagc agctgcccga gaagtacaag 4321 gagatcttct tcgaccagag caagaacggc tacgccggct acatcgacgg cggcgccagc 4381 caggaggagt tctacaagtt catcaagccc atcctggaga agatggacgg caccgaggag 4441 ctgctggtga agctgaaccg cgaggacctg ctgcgcaagc agcgcacctt cgacaacggc 4501 agcatccccc accagatcca cctgggcgag ctgcacgcca tcctgcgccg ccaggaggac 4561 ttctacccct tcctgaagga caaccgcgag aagatcgaga agatcctgac cttccgcatc 4621 ccctactacg tcggccccct ggcccgcggc aacagccgct tcgcctggat gacccgcaag 4681 agcgaggaga ccatcacccc ctggaacttc gaggaggtgg tggacaaggg cgccagcgcc 4741 cagagcttca tcgagcgcat gaccaacttc gacaagaacc tgcccaacga gaaggtgctg 4801 cccaagcaca gcctgctgta cgagtacttc accgtgtaca acgagctgac caaggtgaag 4861 tacgtgaccg agggcatgcg caagcccgcc ttcctgagcg gcgagcagaa gaaggccatc 4921 gtggacctgc tgttcaagac caaccgcaag gtgaccgtga agcagctgaa ggaggactac 4981 ttcaagaaga tcgagtgctt cgacagcgtg gagatcagcg gcgtggagga ccgcttcaac 5041 gccagcctgg gcacctacca cgacctgctg aagatcatca aggacaagga cttcctggac 5101 aacgaggaga acgaggacat cctggaggac atcgtgctga ccctgaccct gttcgaggac 5161 cgcgagatga tcgaggagcg cctgaagacc tacgcccacc tgttcgacga caaggtgatg 5221 aagcagctga agcgccgccg ctacaccggc tggggccgcc tgagccgcaa gctgatcaac 5281 ggcatccgcg acaagcagag cggcaagacc atcctggact tcctgaagag cgacggcttc 5341 gccaaccgca acttcatgca gctgatccac gacgacagcc tgaccttcaa ggaggacatc 5401 cagaaggccc aggtgagcgg ccagggcgac agcctgcacg agcacatcgc caacctggcc 5461 ggcagccccg ccatcaagaa gggcatcctg cagaccgtga aggtggtgga cgagctggtg 5521 aaggtgatgg gccgccacaa gcccgagaac atcgtgatcg agatggcccg cgagaaccag 5581 accacccaga agggccagaa gaacagccgc gagcgcatga agcgcatcga ggagggcatc 5641 aaggagctgg gcagccagat cctgaaggag caccccgtgg agaacaccca gctgcagaac 5701 gagaagctgt acctgtacta cctgcagaac ggccgcgaca tgtacgtgga ccaggagctg 5761 gacatcaacc gcctgagcga ctacgacgtg gaccacatcg tgccccagag cttcctgaag 5821 gacgacagca tcgacaacaa ggtgctgacc cgcagcgaca agaaccgcgg caagagcgac 5881 aacgtgccca gcgaggaggt ggtgaagaag atgaagaact actggcgcca gctgctgaac 5941 gccaagctga tcacccagcg caagttcgac aacctgacca aggccgagcg cggcggcctg 6001 agcgagctgg acaaggccgg cttcatcaag cgccagctgg tggagacccg ccagatcacc 6061 aagcacgtgg cccagatcct ggacagccgc atgaacacca agtacgacga gaacgacaag 6121 ctgatccgcg aggtgaaggt gatcaccctg aagagcaagc tggtgagcga cttccgcaag 6181 gacttccagt tctacaaggt gcgcgagatc aacaactacc accacgccca cgacgcctac 6241 ctgaacgccg tggtgggcac cgccctgatc aagaagtacc ccaagctgga gagcgagttc 6301 gtgtacggcg actacaaggt gtacgacgtg cgcaagatga tcgccaagag cgagcaggag 6361 atcggcaagg ccaccgccaa gtacttcttc tacagcaaca tcatgaactt cttcaagacc 6421 gagatcaccc tggccaacgg cgagatccgc aagcgccccc tgatcgagac caacggcgag 6481 accggcgaga tcgtgtggga caagggccgc gacttcgcca ccgtgcgcaa ggtgctgagc 6541 atgccccagg tgaacatcgt gaagaagacc gaggtgcaga ccggcggctt cagcaaggag 6601 agcatcctgc ccaagcgcaa cagcgacaag ctgatcgccc gcaagaagga ctgggacccc 6661 aagaagtacg gcggcttcga cagccccacc gtggcctaca gcgtgctggt ggtggccaag 6721 gtggagaagg gcaagagcaa gaagctgaag agcgtgaagg agctgctggg catcaccatc 6781 atggagcgca gcagcttcga gaagaacccc atcgacttcc tggaggccaa gggctacaag 6841 gaggtgaaga aggacctgat catcaagctg cccaagtaca gcctgttcga gctggagaac 6901 ggccgcaagc gcatgctggc cagcgccggc gagctgcaga agggcaacga gctggccctg 6961 cccagcaagt acgtgaactt cctgtacctg gccagccact acgagaagct gaagggcagc 7021 cccgaggaca acgagcagaa gcagctgttc gtggagcagc acaagcacta cctggacgag 7081 atcatcgagc agatcagcga gttcagcaag cgcgtgatcc tggccgacgc caacctggac 7141 aaggtgctga gcgcctacaa caagcaccgc gacaagccca tccgcgagca ggccgagaac 7201 atcatccacc tgttcaccct gaccaacctg ggcgcccccg ccgccttcaa gtacttcgac 7261 accaccatcg accgcaagcg ctacaccagc accaaggagg tgctggacgc caccctgatc 7321 caccagagca tcaccggcct gtacgagacc cgcatcgacc tgagccagct gggcggcgac 7381 agccgcgccg accccaagaa gaagcgcaag gtgtgagcgg ccgcatcgat aaaataaaag 7441 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag 7501 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag 7561 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg 7621 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca 7681 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc 7741 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc 7801 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc 7861 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc 7921 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca 7981 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta 8041 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt 8101 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg 8161 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 8221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 8281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 8341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 8401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 8461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 8521 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 8581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 8641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 8701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 8761 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 8821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 8881 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 8941 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 9001 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 9061 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 9121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 9181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 9241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 9301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 9361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 9421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 9481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 9541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 9601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 9661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 9721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 9781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 9841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 9901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 9961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 10021 tgagcggata catatttga //