LOCUS Exported 5988 bp ds-DNA circular SYN 23-AUG-2021 DEFINITION Expresses BMP-2 in mammalian cells. ACCESSION . VERSION . KEYWORDS pCAG-Bmp2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5988) AUTHORS Aihara S, Fujimoto S, Sakaguchi R, Imai T TITLE BMPR-2 gates activity-dependent stabilization of primary dendrites during mitral cell remodeling. JOURNAL Cell Rep. 2021 Jun 22;35(12):109276. doi: 10.1016/j.celrep.2021.109276. PUBMED 34161760 REFERENCE 2 (bases 1 to 5988) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5988 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 5..384 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" intron 663..1679 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1603..1625 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1687..1706 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 1734..1743 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 1738..1747 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(3010..3029) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 3075..3130 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3129..3148) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(3491..3507) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3491..3507) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3504..3526) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3515..3531 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3539..3569) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3584..3605 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3664..3859 /label=SV40 promoter /note="SV40 early promoter" rep_origin 3710..3845 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3772..3791 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 3865..3999 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3902..3921) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3956..3975 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4067..4084) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4238..4826) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4318..4337) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4997..5857) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5620..5639 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5858..5962) /gene="bla" /label=AmpR promoter ORIGIN 1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggnsgcg cgccrggsgg ggsggggsgg ggsgrggggs 541 ggggsggggs gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc 721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg 841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt 901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc 961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg 1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg 1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc 1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc 1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg 1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg 1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg 1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca 1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg 1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc 1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg 1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc 1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgccacca 1741 tggtggccgg gacccgctgt cttctagtgt tgctgcttcc ccaggtcctc ctgggcggcg 1801 cggccggcct cattccagag ctgggccgca agaagttcgc cgcggcatcc agccgaccct 1861 tgtcccggcc ttcggaagac gtcctcagcg aatttgagtt gaggctgctc agcatgtttg 1921 gcctgaagca gagacccacc cccagcaagg acgtcgtggt gcccccctat atgctagatc 1981 tgtaccgcag gcactcaggc cagccaggag cgcccgcccc agaccaccgg ctggagaggg 2041 cagccagccg cgccaacacc gtgcgcagct tccatcacga agaagccgtg gaggaacttc 2101 cagagatgag tgggaaaacg gcccggcgct tcttcttcaa tttaagttct gtccccagtg 2161 acgagtttct cacatctgca gaactccaga tcttccggga acagatacag gaagctttgg 2221 gaaacagtag tttccagcac cgaattaata tttatgaaat tataaagcct gcagcagcca 2281 acttgaaatt tcctgtgacc agactattgg acaccaggtt agtgaatcag aacacaagtc 2341 agtgggagag cttcgacgtc accccagctg tgatgcggtg gaccacacag ggacacacca 2401 accatgggtt tgtggtggaa gtggcccatt tagaggagaa cccaggtgtc tccaagagac 2461 atgtgaggat tagcaggtct ttgcaccaag atgaacacag ctggtcacag ataaggccat 2521 tgctagtgac ttttggacat gatggaaaag gacatccgct ccacaaacga gaaaagcgtc 2581 aagccaaaca caaacagcgg aagcgcctca agtccagctg caagagacac cctttgtatg 2641 tggacttcag tgatgtgggg tggaatgact ggatcgtggc acctccgggc tatcatgcct 2701 tttactgcca tggggagtgt ccttttcccc ttgctgacca cctgaactcc actaaccatg 2761 ccatagtgca gactctggtg aactctgtga attccaaaat ccctaaggca tgctgtgtcc 2821 ccacagagct cagcgcaatc tccatgttgt acctagatga aaatgaaaag gttgtgctaa 2881 aaaattatca ggacatggtt gtggagggct gcgggtgtcg ttaggcggcc gcactcctca 2941 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata 3001 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca 3061 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt 3121 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta 3181 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct 3241 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa 3301 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac 3361 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact 3421 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg 3481 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 3541 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 3601 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga 3661 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 3721 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 3781 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 3841 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa 3901 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3961 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc 4021 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 4081 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 4201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 4261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 4321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 4381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 4441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 4501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 4561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 4621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 4681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 4981 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 5041 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 5101 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 5161 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 5221 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 5281 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 5341 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 5401 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 5461 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 5521 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 5581 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 5641 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5701 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5761 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 5821 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 5881 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5941 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg //