LOCUS Exported 11574 bp ds-DNA circular SYN 21-SEP-2021 DEFINITION Expresses GFP tagged full length TEM4 protein. ACCESSION . VERSION . KEYWORDS pEGFP TEM4 FL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11574) AUTHORS Mitin N, Rossman KL, Der CJ TITLE Identification of a novel actin-binding domain within the Rho guanine nucleotide exchange factor TEM4. JOURNAL PLoS One. 2012;7(7):e41876. doi: 10.1371/journal.pone.0041876. Epub 2012 Jul 24. PUBMED 22911862 REFERENCE 2 (bases 1 to 11574) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11574 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 547..556 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 553..1269 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 553..1269 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(598..619) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(859..878) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1206..1227 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 8302..8423 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8339..8358) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8393..8412 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(8430..8885) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8567..8588) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 8779..8798 /label=F1ori-R /note="F1 origin, reverse primer" promoter 8912..9016 /gene="bla" /label=AmpR promoter promoter 9018..9375 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(9041..9061) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 9226..9361 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9288..9307 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 9410..10204 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9464..9483) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 10074..10093 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(10392..10411) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 10436..10483 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 10812..11400 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11301..11320 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1261 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattcgc cactatggcg 1321 gacggggcac cccggcccca gctttaccgc agcgtctcgt tcaagctgct ggagcgctgg 1381 agcggcggcc ccgggctgag ggaggaggac acggacaccc ccggcttgag gcgacgcgcc 1441 tcgtgccggc cgaccacggc tgcccggggc cagccctctc ggcgcgtgtc caagctggcg 1501 tctgggcccc tggccgcccc cgcgcagccg cgcccgctcc gcagcctctc gccgtcggtt 1561 cgccagctct cccggcgctt cgacgcgccg cgtctggacg acggctccgc tgggacccga 1621 gacggaggcg tcttacccgc ggccgcggaa gaagcggccg agggcccagc gcgaggagcc 1681 tggcccagcg tcaccgagat gcgcaagctc ttcggcggtc ctggctccag gaggcccagc 1741 gccgactctg aatccccagg aacgcccagc cccgacggtg ccgcgtggga gcctccggct 1801 cgggagtcgc ggcagccacc gacgccaccc cctcggacat gcttccccct ggcgggtctg 1861 cgttcggcgc ggcccctgac cgggccggag accgaaggga ggctgcgccg gccgcagcag 1921 caacaggagc gggcgcagcg tccagcggat ggtttacatt cttggcatat cttctcccaa 1981 ccgcaggccg gggcccgggc ctcctgctcc tcctcctcca tcgccgcctc ctatcctgtc 2041 agccgcagtc gtgctgccag ctccagcgag gaggaagagg agggcccgcc gcagctgcct 2101 ggagcccaga gtccggccta ccacggcggc cactcctcgg gcagtgacga cgaccgagac 2161 ggtgagggcg gccaccgctg gggagggagg cccgggctca ggcctggaag ctccctattg 2221 gatcaggact gcaggcctga cagtgatggg ttaaatctaa gcagcatgaa ctcagcaggg 2281 gtttctggga gccctgagcc cccaacatct ccaagagccc ctagagaaga aggactccgg 2341 gagtggggta gtggctctcc gccctgcgtc ccaggtcccc aggagggact tcggcctatg 2401 tctgactctg tgggaggagc tttccgtgtg gccaaggtga gctttccctc gtacctggcc 2461 agccccgcag gctcccgcgg tagcagccgt tattccagca cggagaccct caaggacgac 2521 gacctatggt ctagtagggg ttctgggggc tggggcgtgt accgctcccc tagctttgga 2581 gctggggaag ggctcctgcg gtcccaggct cgaacccgtg ccaaaggacc tggaggcacc 2641 tctagggcat tgagggatgg aggatttgag cctgaaaaga gtcgacagcg gaagtccctg 2701 tcaaatccag atatcgcctc agagaccctg acgcttctca gtttcctgcg ctcagacctt 2761 tcagagctga gggtccgaaa acctggtggg agctccgggg accgtggaag caacccccta 2821 gatggcagag actcaccatc cgcaggtggc cctgtggggc aacttgaacc catacccatc 2881 ccagccccag catcacctgg cacgcgcccc acactcaagg acttgacagc cactctgcgg 2941 agagcaaagt cattcacctg ctctgagaag cccatggccc gccgcctgcc ccgcaccagt 3001 gctctgaagt ccagctcctc cgagctcctg ctcacaggcc ctggtgccga ggaggatccg 3061 ctgcccctca tcgtccagga ccaatatgtg caggaggccc gccaggtttt tgagaagatc 3121 cagcgcatgg gtgcccaaca agatgatgga agcgatgccc cccctggaag ccctgactgg 3181 gcaggggatg tgacccgagg gcagcggtcc caggaggagc tctcaggccc tgagtccagt 3241 ctgacagatg aaggcattgg ggcagaccct gagcctcctg ttgcagcatt ttgcggcctg 3301 ggtaccacag ggatgtggcg acctctttcc tcatcctcgg cccagacgaa ccaccatggc 3361 cctgggactg aggacagtct gggcgggtgg gccctggtgt cgcctgagac ccctcccaca 3421 ccaggtgccc tccgccgacg acgcaaagtc ccaccttcag gttctggtgg gagcgaattt 3481 agcaatgggg aggcagggga ggcctacagg tccctgagtg acccaattcc tcagcgccac 3541 cgggctgcca cctctgaaga gcctactggg ttctctgtgg acagcaacct cctgggctca 3601 ctgagcccca agacagggct ccctgccacc tcagccatgg atgagggctt gaccagtggt 3661 cacagtgact ggtctgtggg cagtgaagag agcaagggat atcaggaggt tattcagagc 3721 atagttcagg ggcctggcac cctggggcgt gtggtggacg acaggattgc tggcaaagcc 3781 cccaagaaga aatccctgag tgaccccagc cgccgtgggg agctggctgg gcctggattc 3841 gagggccctg gaggggagcc catccgagaa gttgagccca tgctgcctcc atccagcagc 3901 gagcccatcc ttgtagagca gcgggcagag ccagaagaac ctggtgccac caggagccgg 3961 gcacagtctg aaagggccct acctgaggct ctgcctcccc ctgccactgc ccaccgaaac 4021 tttcaccttg accccaagct ggctgacatt ctgtccccga ggctaatccg ccgaggctcc 4081 aagaagcgcc cagctcggag tagtcaccag gagcttcgga gagacgaggg cagtcaggac 4141 cagactggca gcctgtctcg ggcccggccc tcctccagac acgttcgcca tgccagtgtg 4201 cccgccacat ttatgcctat tgtggtgcct gagccaccaa cttctgttgg tccccctgtg 4261 gctgtgccag aacccatagg cttccctacc cgagcccatc ccacgttgca ggcaccatcg 4321 ctcgaggacg tcaccaagca gtacatgctg aacctgcact ccggtgaggt ccctgcccca 4381 gtgccagtgg acatgccctg cttgcctctg gctgcaccgc cctctgctga ggccaagccc 4441 cctgaggcag ctcggcctgc agatgagcct acccctgcca gcaagtgctg cagcaagcca 4501 caggtggaca tgcggaagca cgtggccatg accctgctgg acacagagca gtcgtatgtg 4561 gagtcgctgc gcaccctgat gcagggctac atgcagccgc tgaagcagcc agagaactcc 4621 gtgctctgtg acccttcact ggtggacgag atcttcgacc agatccccga gctcctggag 4681 caccacgagc aattcctgga gcaggttcgg cactgcatgc agacctggca tgcccagcag 4741 aaggtgggag ccctgctcgt ccagtcgttc tccaaggatg tcctagtaaa catctattct 4801 gcctatatcg ataacttcct caatgcaaag gatgctgtgc gtgtggccaa ggaggcgagg 4861 cctgcctttc tcaagttcct agagcaaagc atgcgtgaga acaaggagaa gcaggcgctg 4921 tctgacctca tgatcaagcc tgtgcagcgg atcccacgct acgagcttct ggtgaaggac 4981 ctcctgaagc atacacctga ggaccacccg gaccatccac tcctgctgga ggcgcagcgg 5041 aacatcaagc aggtggctga gcgcatcaac aagggtgtgc ggagtgccga ggaggcggag 5101 cgccatgccc gtgtgctgca ggagatagag gctcacatcg agggcatgga ggatctccag 5161 gcccctctgc ggcggttcct gagacaggag atggtcattg aagtgaaggc gatcggtggc 5221 aagaaggacc ggtctctctt cctgttcacg gacctcatcg tctgcaccac tctgaagcga 5281 aagtcaggct ccctgcggcg cagctccatg agcctgtaca cggcagccag tgtcattgac 5341 acagccagca agtacaagat gctgtggaag ctgccgctgg aagacgcaga catcatcaaa 5401 ggggcatccc aagccaccaa tcgggagaac atccagaagg ccatcagccg ccttgatgag 5461 gacctcacca ccctgggcca aatgagcaag ctctctgaga gccttggttt cccccaccag 5521 agcctggacg atgcactgcg ggacctctca gctgccatgc accgggacct gtcggagaag 5581 caggcgctgt gctacgcgct ttccttcccg ccaaccaagc tggagctgtg cgccactcgg 5641 cccgagggca ccgactccta catttttgag ttccctcacc ctgacgcccg ccttggtttt 5701 gaacaggcct tcgatgaggc caagaggaag ctggcatcca gcaaaagctg tctagaccct 5761 gagttcctga aggccatccc catcatgaaa acccgcagtg gcatgcagtt ctcctgtgcg 5821 gctcccaccc tgaacagctg cccggagccc tcgcctgagg tatgggtctg caacagcgac 5881 ggctacgtgg gccaggtgtg cctgctgagc ctgcgcgccg agccggacgt ggaggcctgc 5941 atcgccgtct gttccgcccg catcctctgc atcggggcgg tgcccgggct gcagcctcgc 6001 tgccaccggg agcctcctcc gtcgctgagg agtcctccag agacggcacc ggagcccgcc 6061 gggccggagc tggacgtcga ggccgctgca gacgaggaag ccgcgacgct cgcggagccg 6121 gggccgcagc cctgccttca catctccatt gcaggctcgg gcttggagat gacgccgggc 6181 ctcggcgagg gtgacccccg cccagagctg gtgccctttg acagtgactc tgacgatgag 6241 tcttcgccca gcccctcggg gacgctgcag agccaggcca gccggtccac catctcctcc 6301 agctttggca atgaggagac cccgagttcc aaggaggcca cggcagagac caccagctca 6361 gaggaggagc aggagccagg cttcctgcca ctgtctggct cctttgggcc tggtggtccc 6421 tgcggcacca gcccaatgga tgggagagcc cttcgccgct ccagccacgg ctccttcacc 6481 cggggcagcc ttgaggacct gctgagtgtc gaccctgagg cctaccagag ctccgtgtgg 6541 ctgggcactg aggatggctg tgtccacgtg taccagtcct ccgacagcat ccgtgaccgc 6601 aggaacagca tgaagctcca gcatgcggcc tctgtgacct gcatcttgta tctgaataac 6661 caggtgtttg tgtctctggc caatggagag cttgtggtct accaaaggga agcaggccat 6721 ttctgggacc cccagaactt caaatcagtg accttgggca cccaggggag ccccatcacc 6781 aagatggtat ctgtgggtgg gcggctgtgg tgtggctgcc agaaccgagt ccttgtcctg 6841 agccctgaca cgctgcagct ggagcacatg ttttacgtgg gtcaggattc aagccgctgc 6901 gtggcttgca tggtggactc cagcctgggt gtgtgggtga cattgaaagg tagtgcccac 6961 gtgtgtctct accatccaga cacctttgag cagctggcag aagtagacgt cactcctccc 7021 gtgcacagga tgctggcagg ctcggatgcc atcatccggc agcacaaggc tgcctgtctg 7081 cgaatcacag cgctgctggt gtgtgaggag ctgctgtggg tgggcaccag tgctggtgtc 7141 gtcctcacca tgcccacttc gcccggtact gtcagctgcc cacgggcacc actcagtccc 7201 acaggcctcg gccagggaca caccggccac gtccgcttct tggctgcagt ccagctgcca 7261 gatggcttca acctgctctg cccaacccca ccacctcccc cagacacagg ccccgagaag 7321 ctgccatcac tggagcaccg ggactcccct tggcaccgag gccccgcccc tgccaggcct 7381 aaaatgctgg ttatcagtgg aggtgatggc tatgaggact tccgactcag cagtgggggc 7441 ggcagcagca gtgagactgt gggtcgagac gacagcacaa accacctcct cctgtggagg 7501 gtgtgaccct gtctgccgtg gcccaggact cgcccgccca cctgccttca gcctgcttgc 7561 ctctccctag cccacacgca gactttgacc aggagtatcc agccaggggc acacatgtgc 7621 ctgcgtgggc tctgccttgt cttcgcggaa gcattcctga tggaacaccc actggccagc 7681 caggccatgg cttctcccga ccctctggct gccccggtgc ttccagtcat gatcgggtgg 7741 gggacatgtg ggctgaccag gacctctgac cctggagctt ctaccaaaga cacagctggg 7801 tctggacccc acggggctgg ggagggccat gtgcaatatt tggagggttt tctggagggc 7861 agcaggaagg ctggggaatt ccccatgtac agtatttatg tttcttttta gatgtgtacc 7921 ttcccaagca cttatttatg cagtgacctg gtcacctggg gtgggggtga tttgaggaaa 7981 tgacatgagg aaaagaaacc tattcctgcc ctggggacca ccctgggact ctaaccaagc 8041 cttcctggag ggacccatgc gcccctgagc cccattccat tcatacagac acacacgtac 8101 gcacactgca tgtccaaggc cctaaacatt gcccgttgac ataaactttc cagggcccca 8161 gcctgatggg gctgccctca gtcctctaga taactgatca taatcagcca taccacattt 8221 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 8281 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 8341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 8401 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt 8461 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 8521 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 8581 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 8641 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 8701 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 8761 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 8821 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 8881 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 8941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 9001 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 9061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 9121 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 9181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 9241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 9301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 9361 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 9421 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 9481 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 9541 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 9601 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 9661 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 9721 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 9781 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 9841 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 9901 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 9961 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 10021 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 10081 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 10141 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 10201 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 10261 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 10321 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 10381 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 10441 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 10501 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 10561 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 10621 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 10681 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 10741 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 10801 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 10861 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 10921 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 10981 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 11041 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 11101 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 11161 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 11221 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 11281 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 11341 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 11401 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 11461 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta 11521 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccg //