LOCUS Exported 10964 bp ds-DNA circular SYN 10-SEP-2021 DEFINITION PiggyBac vector to stably express 3xFLAG-tagged AMBRA1. ACCESSION . VERSION . KEYWORDS pPB-AMBRA1-3xFLAG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10964) AUTHORS Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R, Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J TITLE The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D. JOURNAL Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7. Epub 2021 Apr 14. PUBMED 33854239 REFERENCE 2 (bases 1 to 10964) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 10, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10964 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 5..39 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" protein_bind 316..336 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 337..1515 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 568..1506 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1463..1483 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1515..1539 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5188..5253 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" protein_bind complement(5257..5281) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(5359..5378) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 5424..5479 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5478..5497) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" enhancer 5834..6213 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 6214..6417 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 6368..6388 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 6450..7244 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6504..6523) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7114..7133 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(7282..7299) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7288..7512 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 7862..7924 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" primer_bind complement(7990..8009) /label=T7 /note="T7 promoter, forward primer" promoter complement(7991..8009) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8016..8033) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8016..8032) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8025..8047) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8174..8629 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8261..8280) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8471..8492 /label=F1ori-F /note="F1 origin, forward primer" promoter 8655..8759 /gene="bla" /label=AmpR promoter CDS 8760..9620 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8978..8997) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9791..10379 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10280..10299 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10533..10550 /label=L4440 /note="L4440 vector, forward primer" protein_bind 10667..10688 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10703..10733 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10741..10757 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10746..10768 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10765..10781 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10765..10781 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 10802..10820 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc 61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga 121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc 181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg 241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct 301 tgttatagat atcatcaact ttgtatagaa aagttgggct ccggtgcccg tcagtgggca 361 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt 421 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 481 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 541 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc 601 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg 661 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa 721 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg 781 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa 841 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc 901 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg 961 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg 1021 gggtagtctc aagctggccg gcctgctctg gtgcctggtc tcgcgccgcc gtgtatcgcc 1081 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg 1141 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg 1201 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac 1261 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg 1321 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg 1381 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt 1441 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca 1501 tttcaggtgt cgtgacaagt ttgtacaaaa aagcaggctg ccaccatgaa ggttgtccca 1561 gaaaagaatg ctgtccggat actctggggg cgagaacggg gtgctcgggc catgggagct 1621 cagcggcttc tgcaggagct ggtagaggat aaaacccggt ggatgaaatg ggagggcaag 1681 agagtagaac tgccggattc accacgctct accttcttat tggccttcag cccagacagg 1741 actctcttag ccagcaccca tgtgaaccat aatatctata ttacggaggt gaagactggc 1801 aagtgtgttc attccctgat tggacaccgc cgtactccat ggtgtgtcac ttttcatccc 1861 accatctcag gccttattgc ttctggctgc ctagatgggg aggttaggat ttgggattta 1921 cacggtggca gtgaaagctg gttcacagat agcaacaatg ccattgcctc cctggctttc 1981 caccctacgg ctcagctcct gctgattgcc actgccaatg agatccactt ctgggactgg 2041 agtcgacggg aaccctttgc tgtggtgaag acagctagtg agatggaacg ggtccgtctg 2101 gtgagatttg atccacttgg acactactta ctcacagcaa ttgttaaccc ctctaatcaa 2161 cagggtgatg acgaaccaga gatccccata gatggaacag aattatccca ctaccgtcag 2221 cgtgccctcc tgcaatcaca gccagttcgc cggacgcctc tcctccacaa tttcctgcac 2281 atgctgtcct cccgctcttc tggcatccag accgagccct tccatccccc ggagcaggcc 2341 tcgtcaacgc agcaggacca gggcctcctg aaccggccgt ctgccttcag tacagtccag 2401 agcagcactg ccggcaacac gctccgcaac ctcagtctgg gtcctacccg ccgctctttg 2461 ggagggcctc tgtctagcca cccttctagg tatcaccgag aaatagctcc tgggttgaca 2521 ggatctgagt ggacccggac agtactcagt ctgaactccc gctctgaggc ggaatccatg 2581 cccccgccca gaaccagtgc ctcttcggtg agtttgctgt ctgtgctgag acagcaggaa 2641 ggtggctctc aggcatctgt gtacacttca gccacagaag ggaggggttt tccggcatca 2701 gggttggcaa ctgagtcaga tggagggaat ggctccagcc aaaacaactc gggcagcatt 2761 cgccatgagc ttcagtgtga cctgagacgc ttctttctgg agtatgaccg gcttcaggag 2821 ctggatcaga gcctgagtgg ggaagctccc cagacccaac aggcccagga aatgctcaac 2881 aataacattg aatctgagag gccaggccct tcccaccagc ccaccccaca cagcagtgag 2941 aacaactcca acctgtcccg tggccacctg aatcgctgtc gtgcttgcca caatctcctg 3001 accttcaaca acgataccct gcgctgggaa agaaccacac ctaactactc ctctggcgag 3061 gctagttcct cttggcaggt ccccagctcc tttgagagtg tgccatcaag tggcagccag 3121 ttgccacctc tcgagcggac tgagggccaa acgcccagct ccagcaggct ggagttgagc 3181 agctctgcta gtccgcagga ggagaggact gtgggggtgg cctttaacca ggagacaggc 3241 cactgggaaa gaatttacac ccagtccagc agatctggaa ctgtgtcaca ggaggcctta 3301 catcaggata tgcctgagga gagctctgag gaggattcac tcaggaggag gctgctggaa 3361 tcttccctca tttcattatc ccgttatgat ggagcaggat ccagagagca cccaatttac 3421 ccagacccag cgagattatc tcctgctgca tactacgccc agaggatgat ccagtatctc 3481 tcacggagag acagtattcg ccagcgctcc atgcgctacc aacagaaccg tctccgttct 3541 tccacctcct cctcttcctc agacaaccag ggtccatcag tagagggaac cgacttggaa 3601 tttgaggact ttgaggacaa tggtgacaga tccaggcacc gagctccacg caatgcccgg 3661 atgtctgcac cttcgcttgg acgctttgtc ccaaggcgtt tcttgctgcc tgagtacttg 3721 ccttatgctg ggatttttca tgaacgtgga cagcctggct tggctactca ctcttctgtt 3781 aacagggtcc tggcaggggc agtgatcggt gatggacagt ctgctgtggc cagtaacatt 3841 gccaatacta cctaccggct ccagtggtgg gacttcacta agtttgacct ccctgaaatc 3901 agtaatgctt ccgtgaatgt gctggtgcag aactgcaaga tctacaatga tgccagctgt 3961 gacatttctg cagatggcca gctcctggca gctttcatcc ccagcagcca gaggggcttt 4021 cctgatgaag gcatcctggc agtgtactcc ctggcccccc ataacctggg cgaaatgctc 4081 tacaccaagc gatttggtcc caatgccatt tcggtgagcc tgtccccaat gggcagatat 4141 gtaatggtgg gcttggcctc acgaaggatc ctgctgcacc cctccacaga gcacatggtg 4201 gcccaggtct tcaggctgca acaggcccat ggtggagaga cctccatgag gagagttttc 4261 aacgtccttt atcccatgcc tgccgaccag cggagacatg tcagtatcaa ctctgcccgt 4321 tggctgcctg agccagggct tggcttggcc tatggtacta acaaaggaga cctggtgatc 4381 tgccgaccag aggccttaaa ctctggtgtt gagtactact gggaccagct gaacgagacg 4441 gtcttcactg tccattccaa cagcaggagc agcgagcggc ctggaaccag cagagccaca 4501 tggaggacag acagagacat ggggctgatg aatgccattg ggcttcagcc ccggaaccct 4561 gccacctcag tgacatctca gggcacccag actctggccc ttcagctgca gaatgccgaa 4621 acacagactg agagggaggt gccggagcca gggacagccg cctcaggtcc tggtgaaggt 4681 gagggttcag agtatggtgc cagtggagaa gatgcgctca gcaggatcca gaggctgatg 4741 gcggagggcg gcatgacagc cgtggtgcag cgggagcaga gcaccaccat ggcctccatg 4801 ggcggcttcg gcaacaacat catcgtcagc caccgcattc accgcagctc tcagacgggc 4861 actgaacctg gtgccgccca cacctcctca ccccagccct ccacctctcg gggactgctc 4921 ccagaggccg ggcaactggc agagcgaggc ctaagccccc ggacagcttc ctgggaccag 4981 cctggtaccc ctgggcggga gccaacccag ccaaccctgc cctcttcctc ccctgtcccc 5041 attcctgttt cccttcccag cgctgaggga ccaaccctcc actgcgagtt gaccaataac 5101 aaccaccttc tggatggtgg cagcagcagg ggggacgctg caggccctag gggagaacca 5161 cggaacaggg gcagcggcag cggcagcgac tacaaagacc atgacggtga ttataaagat 5221 catgatatcg attacaagga tgacgatgac aagtagaccc agctttcttg tacaaagtgg 5281 tgatcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc 5341 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc 5401 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg 5461 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca 5521 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa 5581 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt 5641 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt 5701 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc 5761 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc 5821 caagcttcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca 5881 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 5941 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 6001 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 6061 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 6121 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 6181 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 6241 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 6301 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 6361 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc 6421 tggctaacta gagaacccac tgcgccacca tgattgaaca agatggattg cacgcaggtt 6481 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 6541 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 6601 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg 6661 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 6721 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 6781 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 6841 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 6901 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 6961 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 7021 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 7081 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 7141 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 7201 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgactcgag tctagagggc 7261 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 7321 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 7381 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 7441 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 7501 tgggctctat ggctcgagtt aattaacgag agcataatat tgatatgtgc caaagttgtt 7561 tctgactgac taataagtat aatttgtttc tattatgtat aggttaagct aattacttat 7621 tttataatac aacatgactg tttttaaagt acaaaataag tttatttttg taaaagagag 7681 aatgtttaaa agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata 7741 aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat 7801 aataaaactt aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac 7861 acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct 7921 agggttaaat aatagtttct aattttttta ttattcagcc tgctgtcgtg aataccgagc 7981 tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt 8041 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 8101 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 8161 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 8221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 8281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 8341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 8401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 8461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 8521 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 8581 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt 8641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 8701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 8761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 8821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 8881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 8941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 9001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 9061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 9121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 9181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 9241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 9301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 9361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 9421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 9481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 9541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 9601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 9661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 9721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 9781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 9841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 9901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 9961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 10021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 10081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 10141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 10201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 10261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 10321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 10381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 10441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 10501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 10561 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 10621 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 10681 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 10741 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg 10801 aaattaaccc tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc 10861 tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc 10921 tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc tttt //