LOCUS Exported 3637 bp ds-DNA circular SYN 13-SEP-2021 DEFINITION Entry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADM. ACCESSION . VERSION . KEYWORDS pME-MmCavin4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3637) AUTHORS Rae J, Ferguson C, Ariotti N, Webb RI, Cheng HH, Mead JL, Riches JD, Hunter DJ, Martel N, Baltos J, Christopoulos A, Bryce NS, Cagigas ML, Fonseka S, Sayre ME, Hardeman EC, Gunning PW, Gambin Y, Hall TE, Parton RG TITLE A robust method for particulate detection of a genetic tag for 3D electron microscopy. JOURNAL Elife. 2021 Apr 27;10. pii: 64630. doi: 10.7554/eLife.64630. PUBMED 33904409 REFERENCE 2 (bases 1 to 3637) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3637 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" regulatory 669..678 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(1762..1861) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1878..1897) /label=T7 /note="T7 promoter, forward primer" promoter complement(1879..1897) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1902..1918) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1902..1918) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2020..2039) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2031..2840 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2098..2117) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2987..3575 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3476..3495 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctgc caccatggaa cacaacggat cagcttcaaa tgctggtaaa atccaccaga 721 atcggttgtc aagtgtgacg gaagatgaag accaagacgc agccctcacc attgtgaccg 781 tgctggacag agtggccagt gtcgtggaca gtgtgcaggc aagccagaag agaattgagg 841 agagacacag agaaatgggt aacgcgataa agtctgtcca aatagacctg ctgaagctct 901 cacagtcaca cagcaatacg ggctacgttg tcaacaagct gtttgagaag acccggaaag 961 tcagcgctca cattaaggat gtcaaggccc gggtagagaa gcaacaggtt cgtgtaacca 1021 aagtcgaaac caagcaagaa gaaataatga agaaaaacaa attccgagtg gtaatcttcc 1081 aggaggacat tccctgcccc gcatccctgt ctgttgttaa agacagaagc ctgcccgaga 1141 accaggagga ggccgaagaa gtcttcgatc ccccaataga gctctcctcg gatgaggagt 1201 attatgttga agaaagcaga tctgccaggc ttaggaagtc aggcaaagag cacattgacc 1261 acatcaagaa agccttttcc agagaaaaca tgcagaagac acggcagact cttgacaaga 1321 aagtgagtgg gattagaact aggatagtca ctcctgagag gagagagagg ctgaggcagt 1381 cgggagagag gctgaggcag tcaggagaga ggctgaggca gtcgggggaa agatttaaga 1441 aatcgatttc cagtgccgct ccctcaaagg aagcttttaa gattcggagc cttaggaaag 1501 cgaaggatcc caaggccgag ggccaggagg tagacagagg gatgggcgtg gacatcatct 1561 caggtagcct ggctctgggg cccatccatg agttccactc tgatgagttc agtgaaacag 1621 aaaaggaggt gaccaaagga gggtacagtc cccaagaagg aggggacccc ccaacgcctg 1681 agcctttgaa ggtgactttt aaaccccagg tgagggtaga ggatgacgag tcactcctgt 1741 tggagctaaa gcagtcctca tacccagctt tcttgtacaa agttggcatt ataagaaagc 1801 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt 1861 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc 1921 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat 1981 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata 2041 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 2101 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga 2161 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 2221 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 2281 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag 2341 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 2401 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 2461 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 2521 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 2581 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 2641 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 2701 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 2761 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 2821 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta 2881 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt 2941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 3001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 3061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 3121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 3181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 3241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 3301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 3361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3601 ggttcctggc cttttgctgg ccttttgctc acatgtt //