LOCUS Exported 6020 bp ds-DNA circular SYN 14-SEP-2021 DEFINITION Expresses mouse syncytin A in mammalian cells. ACCESSION . VERSION . KEYWORDS pCMV-SynA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6020) AUTHORS Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae RK, Koonin EV, Zhang F TITLE Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery. JOURNAL Science. 2021 Aug 20;373(6557):882-889. doi: 10.1126/science.abg6155. PUBMED 34413232 REFERENCE 2 (bases 1 to 6020) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6020 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..204 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 155..175 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 201..225 /label=LNCX /note="Human CMV promoter, forward primer" intron 338..813 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" regulatory 881..890 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 2822..3216 /label=-beta-globin poly(A) /note="human beta-globin polyadenylation signal" primer_bind complement(3555..3572) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3726..4314) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3806..3825) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4485..5345) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5108..5127 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5346..5450) /gene="bla" /label=AmpR promoter primer_bind complement(5520..5539) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" enhancer 5641..6020 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat 241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt 301 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg 361 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag 421 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct 481 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc 541 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat 601 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg 661 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct 721 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta 781 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg 841 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgg ttcgtccttg 901 ggttttctgt ctcctcctgt tcccttgttc ctctgcctac tcggacagct ggatgcccct 961 ggtaaacctc actcaacacc tcctccagga ggctaactct tccttctctt ccaactgctg 1021 ggtctgctta tccatccaaa cccagcgctc tctagccatg ccagccccac taaggacttg 1081 gacagagaca cccatgaaac ttcgaatcat gtactcagcc cggaccctct ccggcccata 1141 ccctatcacc gaccttgaga ggcgcctcca gaatttccaa ccattgactc cccactcctc 1201 ttttgtcaac cctgaccagc gggccattgc tttccttcag atcaccagcg tgacaggcat 1261 acttcccata ctttctcgga tcacctcggt gagatacccc gatgaccacg tctatgaatc 1321 tgcccagcgc cccatatggg gctcactctc cacccagacg atcctcacct cccaggcccc 1381 tctctgcata tcccgcttct tcaagaattc aaaccatgcc accttcgtgg gcaaactccc 1441 tgcctctctt tgcaatcaca cctttcagct ctccccctct gccaaccacc aatccataga 1501 tctgtcctcc agctatgcat tcgccccatt aatggccatg ccagggtcta aatggagaaa 1561 ccccttacgc ttttcaggac ccccttccct gaactcaggg atgcctcact actcctgccc 1621 gatagatgac atccactgcc acacctaccc caccaccccc tggaggtcct gtccttcctt 1681 cccagctagc acctgctata atctcaccct attcgagccg gacaattcga gccaccctat 1741 taccctgtct gtggacacca catacttcaa gattaaactc cagggacaca aagaccccta 1801 tccactcttt cagtaccagc ccctcatggg ggcagccctc tctggacaat attcaatctg 1861 ggaatatgaa cccactgtta agaaaaacgg cggtatcact ccaaatatct tctcccacct 1921 tgtctcctta acatactcct tctgcctcaa ctcctctggt gttttcttcc tctgtggaaa 1981 ctcaacttat gtctgtctcc cggccaattg gtccggcgtc tgtacccttg tcttccaata 2041 cccggatatt gaactccttc ccaacaacca aaccatatct gtcccgcttt ttgctacagt 2101 tccctcctct gtccccgctt ctcgccggaa gcgagccctt cctctccttc ctctcctcgc 2161 gggcctgggc attgcttctg ccctggggtt aggcatcgcg ggtatcacca cctcaactgt 2221 gtatttccaa cagctttcca aggctctctc ggacagccta gatgaaatag ccacctccat 2281 catcagcctc caagaccaaa tagactcgct ggcgggtgtc gttctccaaa accgcagagc 2341 tctggacctc attgtggctg agaggggggg cacctgcctc ttcctccagg aagagtgctg 2401 cttctacata aaccagtccg gggtagtccg gcacgcggca aggaaacttc gagaaagggc 2461 ctcggaactc ggcacaagct cgagttcttg gatccagtgg ctggggctag gaccctggct 2521 gccctcttgg ttgacttccc tcatggctcc cattctcttt atcctggtac tgctggtttt 2581 caggccttgt cttcttaact gcctgactca ttctgtatcg cggcgaatga gttctttcat 2641 tcacaccacc accgaaggac acgtggacaa gatccttctg cttcgagagt cccagtacaa 2701 gagacttccc caagagcccc cggaggagga tgccgtctag gaattcaccc caccagtgca 2761 ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta 2821 agctcgcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta 2881 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat 2941 ttattttcat tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg 3001 tgggaggtca gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa 3061 tacactatat cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg 3121 caacagcccc tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt 3181 gatttggagg ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc 3241 tcatgaatgt cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact 3301 cagttctctt gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc 3361 gagatggttt ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt 3421 ctacttgaag aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct 3481 gcctccccca ctcacagtga cccggaatcc ctcgacatgg cagtctagca ctagtgcggc 3541 cgcagatctg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 3601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 3661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 3721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 3781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 3841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 3901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 3961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 4021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 4081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 4141 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 4201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 4261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 4321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 4381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 4441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 4501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 4561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 4621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 4681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 4741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 4801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 4861 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 4921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 4981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 5041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 5101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 5161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 5221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 5281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 5341 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5461 cgaaaagtgc cacctgacgt ggatcccctg agggggcccc catgggctag aggatccggc 5521 ctcggcctct gcataaataa aaaaaattag tcagccatga gcttggccca ttgcatacgt 5581 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt 5641 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 5701 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 5761 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 5821 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 5881 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 5941 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 6001 tagtcatcgc tattaccatg //