LOCUS Exported 9795 bp ds-DNA circular SYN 23-JUN-2023 DEFINITION Mammalian expression of SpCas9 PEmax prime editor. ACCESSION . VERSION . KEYWORDS pCMV-PEmax SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9795) AUTHORS Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR TITLE Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. JOURNAL Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. PUBMED 34653350 REFERENCE 2 (bases 1 to 9795) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 23, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9795 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 54..433 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 434..637 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 588..608 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 679..698 /label=T7 /note="T7 promoter, forward primer" promoter 679..697 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 747..767 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 768..4868 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 4926..4946 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 7047..7067 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 7077..7103 /codon_start=1 /product="nuclear localization signal of human c-Myc proto-oncogene (Dang and Lee, 1988)" /label=c-myc NLS /translation="PAAKRVKLD" CDS 7112..7129 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(7152..7169) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7158..7382 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7453..7469) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7453..7469) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7466..7488) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7477..7493 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7501..7531) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7546..7567 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7684..7701) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7855..8443) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7935..7954) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8614..9474) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9237..9256 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9475..9579) /gene="bla" /label=AmpR promoter primer_bind complement(9658..9677) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg 61 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 421 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga 481 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca 541 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg 601 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg 661 ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga 721 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca 781 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca 841 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga 901 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga 961 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct 1021 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc 1081 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg 1141 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca 1201 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg 1261 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca 1321 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg 1381 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagaaagctg gaaaatctga 1441 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc 1501 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc 1561 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt 1621 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc 1681 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg 1741 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga 1801 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg 1861 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca 1921 ccgaggaact gctcgtgaag ctgaagagag aggacctgct gcggaagcag cggaccttcg 1981 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc 2041 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct 2101 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga 2161 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg 2221 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga 2281 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca 2341 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa 2401 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag 2461 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc 2521 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact 2581 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt 2641 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca 2701 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc 2761 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg 2821 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag 2881 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca 2941 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg 3001 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag 3061 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag 3121 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc 3181 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc 3241 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct 3301 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca 3361 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc 3421 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag 3481 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc 3541 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga 3601 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt 3661 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg 3721 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa 3781 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg 3841 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt 3901 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa 3961 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag 4021 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca 4081 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact 4141 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg 4201 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga 4261 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg 4321 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc 4381 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac 4441 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga 4501 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc 4561 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta 4621 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg 4681 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt 4741 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca 4801 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg 4861 gaggtgactc cggcggaagc tctggtggca gcaagcggac cgccgacggc tctgaattcg 4921 agagccctaa gaagaaaaga aaggtgagcg gaggctctag cggcggaagc accctgaaca 4981 ttgaagacga gtatagactg catgaaacaa gcaaggaacc cgacgtgtcc ctgggctcca 5041 cctggctgtc cgactttccc caggcctggg ccgagacagg aggaatgggc ctggccgtgc 5101 ggcaggcacc cctgatcatc cctctgaagg ccacctctac acccgtgagc atcaagcagt 5161 accctatgtc tcaggaggcc agactgggca tcaagcctca catccagagg ctgctggacc 5221 agggcatcct ggtgccatgc cagagcccct ggaacacacc actgctgccc gtgaagaagc 5281 caggcaccaa tgactataga cccgtgcagg atctgagaga ggtgaacaag agggtggagg 5341 atatccaccc caccgtgccc aacccttaca atctgctgtc cggcctgccc ccttctcacc 5401 agtggtatac agtgctggac ctgaaggatg ccttcttttg tctgagactg caccctacca 5461 gccagccact gttcgccttt gagtggaggg accctgagat gggcatctct ggccagctga 5521 cctggacacg cctgcctcag ggcttcaaga atagcccaac actgtttaac gaggccctgc 5581 accgcgacct ggcagatttc cggatccagc acccagatct gatcctgctg cagtacgtgg 5641 acgatctgct gctggccgcc accagcgagc tggattgcca gcagggaaca cgcgccctgc 5701 tgcagaccct gggaaacctg ggatataggg catccgccaa gaaggcccag atctgtcaga 5761 agcaggtgaa gtacctgggc tatctgctga aggagggcca gagatggctg acagaggcca 5821 ggaaggagac agtgatgggc cagccaacac ccaagacccc aagacagctg agggagttcc 5881 tgggcaaagc aggattttgc aggctgttca tcccaggatt cgcagagatg gcagcacctc 5941 tgtacccact gaccaagccg ggcaccctgt ttaattgggg ccctgaccag cagaaggcct 6001 atcaggagat caagcaggcc ctgctgacag caccagccct gggcctgcca gacctgacca 6061 agcctttcga gctgtttgtg gatgagaagc agggctacgc caagggcgtg ctgacccaga 6121 agctgggacc atggagacgg cccgtggcct atctgtccaa gaagctggac ccagtggcag 6181 caggatggcc accatgcctg aggatggtgg cagcaatcgc cgtgctgaca aaggatgccg 6241 gcaagctgac catgggacag ccactggtca tcctggcacc acacgcagtg gaggccctgg 6301 tgaagcagcc tccagatcgc tggctgtcta acgcccggat gacacactac caggccctgc 6361 tgctggacac cgatcgcgtg cagtttggcc ctgtggtggc cctgaatcca gccaccctgc 6421 tgcctctgcc agaggagggc ctgcagcaca actgtctgga catcctggca gaggcacacg 6481 gaacaaggcc agacctgacc gatcagcccc tgcctgacgc cgatcacaca tggtataccg 6541 atggaagctc cctgctgcag gagggccaga ggaaggcagg agcagcagtg accacagaga 6601 cagaagtgat ctgggccaag gccctgccag caggcacatc cgcccagcgg gccgagctga 6661 tcgccctgac ccaggccctg aagatggccg agggcaagaa gctgaacgtg tacacagact 6721 ccagatatgc cttcgccacc gcacacatcc acggagagat ctacaggcgc cggggctggc 6781 tgacctctga gggcaaggag atcaagaaca aggatgagat cctggccctg ctgaaggccc 6841 tgtttctgcc caagcggctg agcatcatcc actgtcctgg acaccagaag ggacactccg 6901 ccgaggcaag gggcaatcgg atggccgacc aggccgccag aaaggctgct attactgaaa 6961 ctcccgacac ttccactctg ctgattgaaa actcctcccc ttctggcggc tcaaaaagaa 7021 ccgccgacgg cagcgaattc gagtctccca agaagaagag gaaagtcggc tctggccctg 7081 ccgctaagag agtgaagctg gactaaccgg tcatcatcac catcaccatt gagtttaaac 7141 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 7201 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagaa 7261 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 7321 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 7381 ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt 7441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7501 caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact 7561 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 8041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 8101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 8161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 8221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 8281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8461 ttctacgggg tctgacactc agtggaacga aaactcacgt taagggattt tggtcatgag 8521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 9001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 9061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 9121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 9181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 9241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 9301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 9361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9601 acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac 9661 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt 9721 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 9781 catgaagaat ctgct //