LOCUS Exported 1995 bp ds-DNA linear SYN 07-OCT-2021 DEFINITION DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh beta-strand of GFP added to the C-terminus in yeast plasmid with. ACCESSION . VERSION . KEYWORDS p406 – TDH3-ER-FlucDM-GFP11-Ura SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1995) TITLE ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function JOURNAL Unpublished REFERENCE 2 (bases 1 to 1995) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 7, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1995 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 169..1818 /codon_start=1 /product="firefly luciferase" /label=luciferase /note="synthetic luc2 version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDQDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFQVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" CDS 1855..1881 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 1921..1968 /codon_start=1 /product="11th beta-strand of superfolder GFP" /label=GFP11 /note="associates with GFP(1-10), consisting of the first 10 beta-strands, to reconstitute fluorescent GFP (Cabantous et al., 2005; Kamiyama et al., 2016)" /translation="RDHMVLHEYVNAAGIT" ORIGIN 1 atgtttttca acagactaag cgctggcaag ctgctggtac cactctccgt ggtcctgtac 61 gcccttttcg tggtaatatt acctttacag aattctttcc actcctccaa tgttttagtt 121 agaggtttgg atcctgactt aggtactact tattccttgc tagctgtgat ggaagatgcc 181 aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag 241 cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac 301 gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca 361 gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat 421 agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca 481 gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc 541 gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg 601 atcatacaaa agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg 661 tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag 721 agcttcgacc aggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg 781 cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac 841 cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac 901 cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttca agtcgtgctc 961 atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct 1021 gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac 1081 gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt 1141 gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa 1201 acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag 1261 gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg 1321 aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac 1381 cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc 1441 tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac 1501 aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc 1561 ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc 1621 gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc 1681 caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa 1741 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag 1801 ggcggcaaga tcgccgtgat ccccggggga tcgggtgacg gtccggtcgc cacctaccca 1861 tacgatgttc cagattacgc tggtcgacgg atcggtggtg gttctggagg cggttctatg 1921 agagatcata tggttttgca tgaatatgtt aatgctgctg gtattactaa aaagacccac 1981 gacgaattgt agtaa //