LOCUS Exported 11487 bp ds-DNA circular SYN 13-OCT-2021 DEFINITION Expresses ERBB2. ACCESSION . VERSION . KEYWORDS pLX304-ERBB2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11487) AUTHORS Braunstein EM, Li R, Sobreira N, Marosy B, Hetrick K, Doheny K, Gocke CD, Valle D, Brodsky RA, Cheng L TITLE A germline ERBB3 variant is a candidate for predisposition to erythroid MDS/erythroleukemia. JOURNAL Leukemia. 2016 Nov;30(11):2242-2245. doi: 10.1038/leu.2016.173. Epub 2016 Jun 15. PUBMED 27416908 REFERENCE 2 (bases 1 to 11487) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11487 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 92..113 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 128..158 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 166..182 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 171..193 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 190..206 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 190..206 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 225..245 /label=T3 /note="T3 promoter, forward primer" promoter 227..245 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 273..499 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 500..680 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 727..852 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1345..1578 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1763..1807 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 1982..2482 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 2401..2419 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 2494..2889 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 2949..3066 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 3183..3486 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3487..3690 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3641..3661 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 3713..3737 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7503..7527) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 7529..7570 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7612..8200 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7665..7685) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8083..8094) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8272..8505 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8583..8704 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8620..8639) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8674..8693 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8744..8879 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8806..8825 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8899..8918) /label=T7 /note="T7 promoter, forward primer" promoter complement(8900..8918) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8928..8945) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8928..8944) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8937..8959) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9086..9541 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9173..9192) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9383..9404 /label=F1ori-F /note="F1 origin, forward primer" promoter 9567..9671 /gene="bla" /label=AmpR promoter CDS 9672..10532 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9890..9909) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10703..11291 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11192..11211 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11445..11462 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 181 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact 241 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc 301 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc 361 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg 421 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg 481 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc 541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 661 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca 721 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg 781 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg 841 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta 901 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta 961 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg 1021 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca 1081 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta 1141 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat 1201 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa 1261 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca 1321 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg 1381 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg 1441 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca 1501 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct 1561 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc 1621 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac 1681 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga 1741 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc 1801 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat 1861 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag 1921 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc 1981 cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg 2041 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag 2101 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc 2161 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc 2221 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc 2281 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga 2341 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg 2401 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg 2461 aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc tcaagaagaa 2521 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac 2581 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat 2641 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca 2701 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc 2761 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag 2821 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg 2881 tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat ggcagtattc 2941 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 3001 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 3061 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc 3121 cggggttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 3181 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 3241 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 3301 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 3361 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 3421 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 3481 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 3541 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 3601 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 3661 gtacggtggg aggtctatat aagcagagct ctctggctaa ctgtcgggat caacaagttt 3721 gtacaaaaaa gttggcacca tggagctggc ggccttgtgc cgctgggggc tcctcctcgc 3781 cctcttgccc cccggagccg cgagcaccca agtgtgcacc ggcacagaca tgaagctgcg 3841 gctccctgcc agtcccgaga cccacctgga catgctccgc cacctctacc agggctgcca 3901 ggtggtgcag ggaaacctgg aactcaccta cctgcccacc aatgccagcc tgtccttcct 3961 gcaggatatc caggaggtgc agggctacgt gctcatcgct cacaaccaag tgaggcaggt 4021 cccactgcag aggctgcgga ttgtgcgagg cacccagctc tttgaggaca actatgccct 4081 ggccgtgcta gacaatggag acccgctgaa caataccacc cctgtcacag gggcctcccc 4141 aggaggcctg cgggagctgc agcttcgaag cctcacagag atcttgaaag gaggggtctt 4201 gatccagcgg aacccccagc tctgctacca ggacacgatt ttgtggaagg acatcttcca 4261 caagaacaac cagctggctc tcacactgat agacaccaac cgctctcggg cctgccaccc 4321 ctgttctccg atgtgtaagg gctcccgctg ctggggagag agttctgagg attgtcagag 4381 cctgacgcgc actgtctgtg ccggtggctg tgcccgctgc aaggggccac tgcccactga 4441 ctgctgccat gagcagtgtg ctgccggctg cacgggcccc aagcactctg actgcctggc 4501 ctgcctccac ttcaaccaca gtggcatctg tgagctgcac tgcccagccc tggtcaccta 4561 caacacagac acgtttgagt ccatgcccaa tcccgagggc cggtatacat tcggcgccag 4621 ctgtgtgact gcctgtccct acaactacct ttctacggac gtgggatcct gcaccctcgt 4681 ctgccccctg cacaaccaag aggtgacagc agaggatgga acacagcggt gtgagaagtg 4741 cagcaagccc tgtgcccgag tgtgctatgg tctgggcatg gagcacttgc gagaggtgag 4801 ggcagttacc agtgccaata tccaggagtt tgctggctgc aagaagatct ttgggagcct 4861 ggcatttctg ccggagagct ttgatgggga cccagcctcc aacactgccc cgctccagcc 4921 agagcagctc caagtgtttg agactctgga agagatcaca ggttacctat acatctcagc 4981 atggccggac agcctgcctg acctcagcgt cttccagaac ctgcaagtaa tccggggacg 5041 aattctgcac aatggcgcct actcgctgac cctgcaaggg ctgggcatca gctggctggg 5101 gctgcgctca ctgagggaac tgggcagtgg actggccctc atccaccata acacccacct 5161 ctgcttcgtg cacacggtgc cctgggacca gctctttcgg aacccgcacc aagctctgct 5221 ccacactgcc aaccggccag aggacgagtg tgtgggcgag ggcctggcct gccaccagct 5281 gtgcgcccga gggcactgct ggggtccagg gcccacccag tgtgtcaact gcagccagtt 5341 ccttcggggc caggagtgcg tggaggaatg ccgagtactg caggggctcc ccagggagta 5401 tgtgaatgcc aggcactgtt tgccgtgcca ccctgagtgt cagccccaga atggctcagt 5461 gacctgtttt ggaccggagg ctgaccagtg tgtggcctgt gcccactata aggaccctcc 5521 cttctgcgtg gcccgctgcc ccagcggtgt gaaacctgac ctctcctaca tgcccatctg 5581 gaagtttcca gatgaggagg gcgcatgcca gccttgcccc atcaactgca cccactcctg 5641 tgtggacctg gatgacaagg gctgccccgc cgagcagaga gccagccctc tgacgtccat 5701 catctctgcg gtggttggca ttctgctggt cgtggtcttg ggggtggtct ttgggatcct 5761 catcaagcga cggcagcaga agatccggaa gtacacgatg cggagactgc tgcaggaaac 5821 ggagctggtg gagccgctga cacctagcgg agcgatgccc aaccaggcgc agatgcggat 5881 cctgaaagag acggagctga ggaaggtgaa ggtgcttgga tctggcgctt ttggcacagt 5941 ctacaagggc atctggatcc ctgatgggga gaatgtgaaa attccagtgg ccatcaaagt 6001 gttgagggaa aacacatccc ccaaagccaa caaagaaatc ttagacgaag catacgtgat 6061 ggctggtgtg ggctccccat atgtctcccg ccttctgggc atctgcctga catccacggt 6121 gcagctggtg acacagctta tgccctatgg ctgcctctta gaccatgtcc gggaaaaccg 6181 cggacgcctg ggctcccagg acctgctgaa ctggtgtatg cagattgcca aggggatgag 6241 ctacctggag gatgtgcggc tcgtacacag ggacttggcc gctcggaacg tgctggtcaa 6301 gagtcccaac catgtcaaaa ttacagactt cgggctggct cggctgctgg acattgacga 6361 gacagagtac catgcagatg ggggcaaggt gcccatcaag tggatggcgc tggagtccat 6421 tctccgccgg cggttcaccc accagagtga tgtgtggagt tatggtgtga ctgtgtggga 6481 gctgatgact tttggggcca aaccttacga tgggatccca gcccgggaga tccctgacct 6541 gctggaaaag ggggagcggc tgccccagcc ccccatctgc accattgatg tctacatgat 6601 catggtcaaa tgttggatga ttgactctga atgtcggcca agattccggg agttggtgtc 6661 tgaattctcc cgcatggcca gggaccccca gcgctttgtg gtcatccaga atgaggactt 6721 gggcccagcc agtcccttgg acagcacctt ctaccgctca ctgctggagg acgatgacat 6781 gggggacctg gtggatgctg aggagtatct ggtaccccag cagggcttct tctgtccaga 6841 ccctgccccg ggcgctgggg gcatggtcca ccacaggcac cgcagctcat ctaccaggag 6901 tggcggtggg gacctgacac tagggctgga gccctctgaa gaggaggccc ccaggtctcc 6961 actggcaccc tccgaagggg ctggctccga tgtatttgat ggtgacctgg gaatgggggc 7021 agccaagggg ctgcaaagcc tccccacaca tgaccccagc cctctacagc ggtacagtga 7081 ggaccccaca gtacccctgc cctctgagac tgatggctac gttgcccccc tgacctgcag 7141 cccccagcct gaatatgtga accagccaga tgttcggccc cagccccctt cgccccgaga 7201 gggccctctg cctgctgccc gacctgctgg tgccactctg gaaaggccca agactctctc 7261 cccagggaag aatggggtcg tcaaagacgt ttttgccttt gggggtgccg tggagaaccc 7321 cgagtacttg acaccccagg gaggagctgc ccctcagccc caccctcctc ctgccttcag 7381 cccagccttc gacaacctct attactggga ccaggaccca ccagagcggg gggctccacc 7441 cagcaccttc aaagggacac ctacggcaga gaacccagag tacctgggtc tggacgtgcc 7501 agtgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc tcctcggtct 7561 cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct 7621 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 7681 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 7741 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 7801 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 7861 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 7921 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 7981 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt 8041 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 8101 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 8161 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga 8221 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 8281 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag 8341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 8401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 8461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt 8521 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 8581 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 8641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 8701 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc 8761 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 8821 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 8881 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 8941 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 9001 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 9061 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 9121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 9181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 9241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 9301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 9361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 9421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 9481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 9541 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 9601 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 9661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 9721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 9781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 9841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 9901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 9961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 10021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 10081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 10141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 10201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 10261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 10321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 10381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 10441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 10501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 10561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 10621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 10681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 10741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 10801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 10861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 10921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 10981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 11041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 11101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 11161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 11221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 11281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 11341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 11401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 11461 aggaagcgga agagcgccca atacgca //