LOCUS Exported 6126 bp ds-DNA circular SYN 18-OCT-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pNeuLite SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6126) AUTHORS Xing X, Wang SC, Xia W, Zou Y, Shao R, Kwong KY, Yu Z, Zhang S, Miller S, Huang L, Hung MC TITLE The ets protein PEA3 suppresses HER-2/neu overexpression and inhibits tumorigenesis. JOURNAL Nat Med. 2000 Feb . 6(2):189-95. PUBMED 10655108 REFERENCE 2 (bases 1 to 6126) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6126 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 137..258 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS 887..2539 /codon_start=1 /gene="luc" /product="firefly luciferase" /label=luciferase /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" primer_bind complement(953..971) /label=LucNrev /note="Firefly luciferase, reverse primer" intron 2780..2845 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2975..2995 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 3420..3554 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3696..3713) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3867..4455) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3947..3966) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4626..5486) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5249..5268 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5487..5591) /gene="bla" /label=AmpR promoter rep_origin 5618..6073 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5705..5724) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5915..5936 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgtgga 61 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 121 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 181 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 241 actcatcaat gtatcttatg gtactgtaac tgagctaaca taacccggga ggtaccgagc 301 tcttacgcgt gctagctcga gatccggggg tcctggaagc cacgaggtaa acacaacaca 361 tccccctcct tgactatcaa ttttactaga ggatgtggtg ggaaaaccat tatttgatat 421 taaaacaaat aggcttggga tggagtagga tgcaagctcc ccaggaaagt ttaagataaa 481 acctgagact taaaagggtg ttaagagtgg cagcctaggg aatttatccc ggactccggg 541 ggagggggca gagtcaccag cctctgcatt tagggattct ccgaggaaaa gtgtgagaac 601 ggctgcaggc aacccaggcg tcccggcgct aggagggacg cacccaggcc tgcgcgaaga 661 gagggagaaa gtgaagctgg gagttgccac tcccagactt gttggaatgc agttggaggg 721 ggcgagctgg gagcgcgctt gctcccaatc acaggagaag gaggaggtgg aggaggaggg 781 ctgcttgagg aagtataaga atgaagttgt gaagctgaga ttcccctcca ttgggaccgg 841 agaaaccaga tctaagtaag cttggcattc cggtactgtt ggtaaaatgg aagacgccaa 901 aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg ctggagagca 961 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc 1021 acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc ggttggcaga 1081 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc 1141 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc 1201 gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc agcctaccgt 1261 agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat 1321 aatccagaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta 1381 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg taccagagtc 1441 ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta ctgggttacc 1501 taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc 1561 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca 1621 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat 1681 gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa ttcaaagtgc 1741 gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg acaaatacga 1801 tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag aagtcgggga 1861 agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc tcactgagac 1921 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt 1981 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa 2041 tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg taaacaatcc 2101 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta 2161 ctgggacgaa gacgaacact tcttcatagt tgaccgcttg aagtctttaa ttaaatacaa 2221 aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc ccaacatctt 2281 cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt 2341 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca 2401 agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg 2461 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg 2521 cggaaagtcc aaattgtaaa atgtaactgt attcagcgat gacgaaattc ttagctattg 2581 taatactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct 2641 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg 2701 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac 2761 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact 2821 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg 2881 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga 2941 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga 3001 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag 3061 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa 3121 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat 3181 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa 3241 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag 3301 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa 3361 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta 3421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 3481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 3541 atcatgtctg gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc 3601 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 3661 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 3721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 3781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 3841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 3901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 4021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 4081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 4141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 4201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 4261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 4321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 4381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 4441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 4501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 4561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 4621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 5041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 5101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 5161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 5221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 5281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 5341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 5401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 5461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 5521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 5581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 5641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 5701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 5761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 5821 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 5881 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 5941 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 6001 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 6061 acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6121 cgggcc //