LOCUS       Exported               10145 bp ds-DNA     circular SYN 19-OCT-2021
DEFINITION  A block for the AdenoBuilder genome assembly system. The insert 
            consolidates the regions present in pAd5-B3 and pAd5-B4..
ACCESSION   .
VERSION     .
KEYWORDS    pAd5-B3/4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10145)
  TITLE     Adenobuilder
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4142..4153
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(7977..8007)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    8022..8043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8163..8180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8334..8922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8414..8433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9093..9953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9716..9735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9954..10058)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10126..10144
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tgttcgaagt tgatgtcttc cattctacaa aatagttaca ggaccaagcg
      421 agcgtgagag tccagacttt ttattttgat ttttccacat gcaacttgtt tttaatcagt
      481 gtctctgcgc ctgcaaggcc acggatgcaa ttccgggcac ggcgccaatc gccgcggcga
      541 tcagtggaat aaggaggggc aggataccgc cgcgcatgcg acggtgcgac gcgcgccgcc
      601 gccggtggtg cgcacgacgc atgccgcccg tcaggccgtg gccggccatg cccctcctac
      661 ggtgcattct tcctcggaat cccggcaccg ggaaacggag gcggcaggtg agggccatat
      721 ctgcaagaac cacaaagacc ggcttttaaa cgatgctggg gtggtagcgc gctgttggca
      781 gcaccagggt cctgcctcct tcgcgagcca ccctgcgcac ggaaatcggg gccagcacgg
      841 gctggcgacg gcgacggcgg cggcgggttc cagtggtggt tcggcgtcgg gtagttgctc
      901 gtcttctggg gcggtaggtg tagccacgat agccgggggt aggcgcaatg gaaggatgta
      961 gggcatattc gggcagtagc gcgctggcgg cgccgtactt cctcgaacgg cgcgggcgcc
     1021 ggggggctga aacgcgaaac atccacgggt ccgtttgcac ctccgtagag gtcttggacg
     1081 cggccgcagc gaccgcctgc accgcggcat ccgccaccgc tgaggcaacc ggggacgttt
     1141 gtgtctccat gccctctgtg gcggtggcaa tactggtgct actggtagtg ggtatctgaa
     1201 cgtccacggt ctgcacgccc agtcccggcg ccacctgctt gattggccgc acgcggacct
     1261 cgggctccag cccaggttcc acggtcattt tttccaagac atcttccagt cgctggcgct
     1321 tgggtaccat cagctgcacg gtgggtgcca agtcaccaga ctcgcgcttt aggccgcgct
     1381 tttcttcgga cggtgcaagc gcgggcagca cctgctgcag tgttacgggc tttaggctag
     1441 gtgttgggtt gccctcgtcc agcggcaacg ccagcatgtc cttatgccgc tttccgtagg
     1501 caaactcccc gaggcgctcg ttggcctgct caagcaggtc ctcgtcgccg tacacctcat
     1561 catacacgcg cttgtaggtg cgggtggagc gctcaccggg cgtaaagact acggtggtgc
     1621 cgggtcgcaa aacacgtttt acgcgtcgac ctttccactg tacccgtcgc ctgggcgcgg
     1681 tagcgtgcag cagttccacc tcgtcgtcaa gttcatcatc atcatctttc tttttctttt
     1741 tgacccgctt tagctttcgg ggcttgtaat cctgctcttc cttcttcggg gggccataga
     1801 tctccggcgc gatgacctgg agcatctctt ctttgatttt gcgcttggac atagcttcgt
     1861 tgcgcgccgc cgccgctgga tacatacaac agtacgagtc taagtagttt tttcttgcaa
     1921 tctagttgcg cggggggcgg gtgcgcacgg gcacgcgcag gccgctaacc gagtcgcgca
     1981 cccaatacac gttgcccctg cgaccctgag tcatagcact aatggccgcg gctgctgcgg
     2041 cggccgctcg tcgcctggac ctggggggca cagtgacaat acccgcggcc agccttcgag
     2101 cggcccgcat ggccgcccgt cggccggtgc gacgtgcgcg gttaagcagg gccgccgccg
     2161 cgcgttgggc ggcagtgccg ggtcggcggc ggtggcgacg tgctacgcgc ctccgccgtc
     2221 tcttcatttt agcatagcgc cgggctccgc gcaccacggt ctgaatggcc gcgtccactg
     2281 tggacactgg tggcggcgtg ggcgtgtagt tgcgcgcctc ctccaccacc gcgtcgatgg
     2341 cgtcatcgac ggtggtgcgc ccagtgcggc cgcgtttgtg cgcgccccag ggcgcgcggt
     2401 agtgcccgcg cacgcgcact gggtgttggt cggagcgctt cttggccccg ccaaacatct
     2461 tgcttgggaa gcgcaggccc cagcctgtgt tattgctggg cgatataagg atggacatgc
     2521 ttgctcaaaa agtgcggctc gataggacgc gcggcgagac tatgcccagg gccttgtaaa
     2581 cgtaggggca ggtgcggcgt ctggcgtcag taatggtcac tcgctggact cctccgatgc
     2641 tgttgcgcag cggtagcgtc ccgtgatctg tgagagcagg aacgttttca ctgacggtgg
     2701 tgatggtggg ggctggcggg cgcgccaaaa tctggttctc gggaaagcga ttgaacacgt
     2761 gggtcagaga ggtaaactgg cggatgagtt gggagtagac ggcctggtcg ttgtagaagc
     2821 tcttggagtg cacgggcaac agctcggcgc ccaccaccgg aaagttgctg atctggcgcg
     2881 tggagcggaa ggtcacgggg tcttgcatca tgtctggcaa cgaccagtag acctgctccg
     2941 agccgcaggt tacgtcagga gtgcaaagca gggtccatga gcggattccg gtctgagggt
     3001 cgccgtagtt gtatgcaagg taccagctgc ggtactgggt gaaggtgctg tcattgctta
     3061 ttaggttgta actgcgtttc ttgctgtcct ctgtcagggg tttgatcacc ggtttcttct
     3121 gaggcttctc gacctcgggt tgcgcagcgg gggcggcagc ttcggccgct gcttcggcct
     3181 cagcgcgctt ctcctcagcc cgtgtggcaa aggtgtcgcc gcgaatggca tgatcgttca
     3241 tgtcctccac cggctgcatt gccgcggctg ccgcgttgga gttctcttcc gcgccgctgc
     3301 cactgctgtt gctgccgcct gcgccacccc cgccctgttc ggtgtcatct ttcaagctcg
     3361 cctggtaggc gtccacatcc aacagtgcgg gaatgttacc accctccaga tcatcgtagg
     3421 tgatcctaaa gccctcctgg aagggttgcc gcttgcggat gcccaacaag ttgctcaggc
     3481 ggctgtgggt gaagtccacc ccgcatcctg gcagcaaaat gatgtctgga tggaaggctt
     3541 cgtttgtata taccccaggc atgacaagac cagtgacggg gtcaaacccc agtctgaagt
     3601 tgcgggtgtc aaactttacc ccgatgtcgc tttccagaac cccgttctgt ctgcccactt
     3661 tcaagtagtg ctccacgatc gcgttgttca taaggtctat ggtcatggtc tcggagtagt
     3721 tgccctcggg cagcgtgaac tccacccact cgtatttcag ctccacctga ttgtccttag
     3781 taggcaagcg cgacaccatc acccgcgcct taaacttatt ggtaaacatg aactcgttca
     3841 catttggcat gttggtatgc aggatggttt tcaggtcgcc gccccagtgc gaccggtcgt
     3901 caagattgat ggtctgtgtg cttgcctccc ccgggctgta gtcattgttt tgaatgaccg
     3961 tggtcagaaa gttgctgtgg tcgttctggt agttcaggga tgccacatcc gttgacttgt
     4021 tgtccaccag gtacacacgg gtggtgtcga ataggggtgc caactcagag taacggatgc
     4081 tgtttctccc cccggtaggc cgcaggtacc gcggaggcac aaacggcggg tccaggggag
     4141 catcgaaggg agaacccagc gccgccgcca ctggcgccgc gctcaccaca ctctcgtagg
     4201 agggaggagg accttcctca tacatcgccg cgcgccgcat actaagggga atacaagaaa
     4261 accaacgctc ggtgccatgg ccttggtgag ttttttattt tgcatcatgc tttttttttt
     4321 tttaaaacat tctccccagc ctggggcgaa ggtgcgcaaa cgggttgcca ctccctccca
     4381 aatccaggac gctgctgtcg tctgccgagt catcgtcctc ccacaccaga ccccgctgac
     4441 ggtcgtgcct ttgacgacgg gtgggcgggc gcgggcctgg cacgtccctg tgctcctgcg
     4501 cgtacgtctt ccatctactc atcttgtcca ctaggctctc tatcccgttg ttgggaaatg
     4561 ccggaggcag gtttttttcg cgctgcggct gcagcagcga gttgtttagg tactcctcct
     4621 cgcccagcag gcgcgggcgg gtggtgcgag tgctggtaag agaccctatc aagcttggaa
     4681 atgggctact agcatctgac cgcggggccg cagcgcctag atcggacaag ctgcttggcc
     4741 tgcggaagct ttcctttcgc agcgccgcct ctgcctgctc gcgctgttgc aactctagca
     4801 gggtctgcgg ttgcggggaa aacacgctgt cgtctatgtc gtcccagagg aatccatcgt
     4861 taccctcggg cacctcgaat cccccggtgt agaaaccagg gggcggtagc cagtgcgggt
     4921 tcaagatggc attggtgaaa tactcggggt tcacggcggc cgcgcgatgc aagtagtcca
     4981 ttaggcggtt gataaacggc cggtttgagg catacatgcc cggttccatg ttgcgcgcgg
     5041 tcatgtccag cgccacgctg ggcgttaccc cgtcgcgcat caggttaagg ctcacgctct
     5101 gctgcacgta gcgcaaaatg cgctcctcct cgctgtttaa actgtgcaac gaggggatct
     5161 tctgccgccg gttggtcagc aggtagttta gggttgcctc caggctgccc gtgtcctcct
     5221 gccccagcgc gcggctgaca cttgtaatct cctggaaagt atgctcgtcc acatgcgcct
     5281 gacctatggc ctcgcggtac agtgtcagca agtgacctag gtatgtgtcc cgggacacgc
     5341 tgccactgtc cgtgaagggc gctattagca gcagcaacag gcgcgagttg ggcgtcagca
     5401 agctagacac ggtcgcgcgg tcgcctgtgg gagcccgcac cccccacagc ccctgcaagt
     5461 ttttgaaagc ctggctcagg tttacggtct gcaggccttg tctactggtc tggaaaaaat
     5521 agtctggccc agactggtac acctcacttt gcggtgtctc agtcaccatt agccgcagtg
     5581 cgctcacaaa gttggtgtag tcctcctgtc cccgcggcac gttggcgggc tgtgtactca
     5641 ggaaggcgtt tagtgcaacc atggagccca ggttgccctg ctgctgcgcg cgctcacgct
     5701 gcgccacggc ctcgcgcaca tcccccacca gccggtccag gttggtctgc acgttgccgc
     5761 tgttgtaacg agccacgcgc tgaagcagcg cgtcgtagac caggccggcc tcgtcgggcc
     5821 ggatggccct gttttcggcc agcgcgttta cgatcgccag caccttctcg tgcgtggggt
     5881 ttgcgcgcgc cgggaccacc gcttccagaa ttgcggagag ccggttggcc tgcggctgct
     5941 gccggaacgc gtcaggattg cgcgcagtca gcgacatgat gcggtccatg acctggcgcc
     6001 agtcgtccgt ggagttaagg ccggacggct ggctctgcag cgccgcccgc accgccgggt
     6061 ccgttgcgtc ttgcatcatc tgatcagaaa catcaccgct tagtactcgc cgtcctctgg
     6121 ctcgtactca tcgtcctcgt catattcctc cacgccgccg acgttgccag cgcgcgcggg
     6181 tgccaccgcc agcccaggtc cggccccagc tgcctccagg gcgcgtcggc ttggggccca
     6241 gcgcaggtca gcgcccgcgt caaagtagga ctcggcctct ctatcgccgc tgcccgtgcc
     6301 agccagggcc ctttgcaggc tgtgcatcag ctcgcggtcg ctgagctcgc gccgccggct
     6361 cacgctcacg gccttgtgga tgcgctcgtt gcgataaacg cccaggtcgt cgctcaaggt
     6421 aagcaccttc agcgccatgc gcatgtagaa cccctcgatc tttacctcct tgtctatggg
     6481 aacgtaaggg gtatggtata tcttgcgggc gtaaaacttg cccaggctaa gcatggaata
     6541 gttgatggcg gccaccttgt cagccaggct caagctgcgc tcctgcacca ctatgctctg
     6601 caggatgttt atcaaatcga gcagccagcg gccctcgggc tctactatgt ttagcagcgc
     6661 atccctgaat gcctcgttgt ccctgctgtg ctgcactata aggaacagct gcgccatgag
     6721 cggcttgcta tttgggtttt gctccagcgc gcttacaaag tcccacagat gcatcagtcc
     6781 tatagccacc tcctcgcgcg ccacaagcgt acgcacgtgg ttgttaaagc ttttttgaaa
     6841 gttaatctcc tggttcaccg tctgctcgta tgcggttacc aggtcggcgg ccgccacgtg
     6901 tgcgcgcgcg ggactaatcc cggttcgcgc gtcgggctca aagtcctcct cgcgcagcaa
     6961 ccgctcgcga ttcaggccat gccgcagctc gcgccctgcg tggaactttc gatcccgcat
     7021 ctcctcgggc tcctctccct cgcggtcgcg aaacaggttc tgccgcggca cgtacgcctc
     7081 acgcgtatca cgcttcagct gcacccttgg gtgccgctca ggagagggcg ctcctagccg
     7141 cgccaggccc tcgccctcct ccaagtccag gtagtgccgg gcccggcgcc gcgggggttc
     7201 gtaatcacca tctgctgccg cgtcaaccgc ggatgtcgcc cctcctgacg cggtaggagg
     7261 aggggagggt gccctgcatg tctgccgctg ctcttgctct tgccgctgct gaggaggggg
     7321 gcgcatctgc cgcagcaccg gatgcatctg ggaaaagcaa aaaaggggct cgtccctgtt
     7381 tccggaggaa tttgcaagcg gggtcttgca tgacggggag gcaaaccccc gttcgccgca
     7441 gtccggccgg tccgagactc gaaccggggg tcccgcgact caacccttgg aaaataaccc
     7501 tccggctaca gggagcgagc cacttaatgc tttcgttcga agatctttct agaagatctc
     7561 ctacaatatt ctcagctgcc atggaaaatc gatgttcttc ttttattctc tcaagatttt
     7621 caggctgtat attaaaactt atattaagaa ctatgctaac cacctcatca ggaaccgttg
     7681 taggtggcgt gggttttctt ggcaatcgac tctcatgaaa actacgagct aaatattcaa
     7741 tatgttcctc ttgaccaact ttattctgca ttttttttga acgaggttta gagcaagctt
     7801 caggaaactg agacaggaat tttattaaaa atttaaattt tgaagaaagt tcagggttaa
     7861 tagcatccat tttttgcttt gcaagttcct cagcattctt aacaaaagac gtctcttttg
     7921 acatgtttaa agtttaaacc tcctgtgtga aattattatc cgctcataat tccacacatt
     7981 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8041 ttaattgcgt tgcgctcact gccaattgct ttccagtcgg gaaacctgtc gtgccagctg
     8101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8221 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8281 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8341 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8401 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8461 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8521 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8581 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8641 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8701 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8761 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8821 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8881 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8941 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9001 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9061 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9121 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9181 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9241 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9301 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9361 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9421 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9481 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9541 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9601 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9661 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9721 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9781 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9841 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9901 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9961 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10021 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10081 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10141 aggcc
//
