LOCUS Exported 5110 bp ds-DNA circular SYN 25-OCT-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCFJ90 - Pmyo-2::mCherry::unc-54utr SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5110) AUTHORS Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, TITLE Single-copy insertion of transgenes in Caenorhabditis elegans PUBMED 18953339 REFERENCE 2 (bases 1 to 5110) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5110 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(71..91) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(99..116) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(99..115) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(108..130) /label=M13/pUC Forward /note="In lacZ gene" primer_bind 443..465 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(503..521) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 589..693 /gene="bla" /label=AmpR promoter CDS 694..1554 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(912..931) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1725..2313 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2214..2233 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2440..2462 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2459..2475 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2459..2475 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind 2495..2515 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 3495..3519 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4387..4411) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca 61 taactgtttc caactttatt atacatagtt gataattcac tggccgtcgt tttacaacgt 121 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 181 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 241 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 301 caccgcatat ggtgcactct cagtgcaatc tgctctgatg ccgcatagtt aagccagccc 361 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 421 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 481 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 541 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 601 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 661 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 721 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 781 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 841 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 901 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 961 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 1021 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 1081 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 1141 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 1201 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 1261 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 1321 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 1381 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggtca 1441 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 1501 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 1561 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 1621 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 1681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 1741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 1801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 1861 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 1921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 1981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 2041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2341 ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 2401 gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca atttcacaca 2461 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttgcattt 2521 tatatctgag tagtatcctt tgctttaaat gtccataaaa ctaattttat aatcaataaa 2581 acaacgtttg taaatcaact gagtttacaa gtagagacat tgagggatac tttcactatg 2641 ctaaagtgat aatcgaccaa ataataactt cactttggta tttattcctg tcttataaat 2701 gttatgtatg aattaaattc atatgcatat ggctcactct gacaattttt aataatcttc 2761 cagatcaata ttgactaccg atgcgggtgg tcttttgctt tgaattctgc tgaactttac 2821 accccgaaca gcaatgtgtg cttcagccta aaaaaaagta agtgtgttaa tcagtgcccc 2881 cgattcttca ttttttgccc ctctctcccg tttcgtcggc aaaagaagag aaaataaaga 2941 taagtctcaa gataggttgg taatcgctaa agtggttgtg tggataagag tagcaaaatg 3001 gcaggaagag cactttgcgc gcacacactg tactcattgt tctggataaa attctctcgt 3061 tgtttgccgt cggatgtctg cctctctgca ttgagccggc ttcttcacta tctttagtta 3121 acctaaaatg ccgtttcttt tctcgtatcc ccactatccc gttgaggttc tctgctctct 3181 tcgctcccta ccgccagcga gcaactatcc gtgggggcgc cttgctcgga agatgggggg 3241 gaagaaagaa gatttttgct atttgcactt gagaaagaga cttttcctgc gtcgatggtc 3301 agagaacagt gtgcagacac ttttcagcta cctagaatta caattggata tccccgcctc 3361 ccaatccacc cacccaggga aaaagaaggg ctcgccgaaa atcaaagtta tctccaggct 3421 cgcgcatccc accgagcggt tgacttctct ccaccacttt tcattttaac cctcgatcgt 3481 cagacacaga aatgacaagt ttgtacaaaa aagcaggctt aatggtctca aagggtgaag 3541 aagataacat ggcaattatt aaagagttta tgcgtttcaa ggtgcatatg gagggatctg 3601 tcaatgggca tgagtttgaa attgaaggtg aaggagaagg ccgaccatat gagggaacac 3661 aaaccgcaaa actaaaggta agtttaaaca tatatatact aactaaccct gattatttaa 3721 attttcaggt aactaaaggc ggaccattac cattcgcctg ggacatcctc tctccacagt 3781 tcatgtatgg aagtaaagct tatgttaaac atccggcaga tataccagat tatttgaaac 3841 tttcattccc ggagggtttt aagtgggaac gcgtaatgaa ttttgaagac ggaggagttg 3901 ttacagtgac gcaagactca aggtaagttt aaacagttcg gtactaacta accatacata 3961 tttaaatttt cagcctccaa gatggagaat ttatttataa agtcaaactt cgaggaacga 4021 atttcccctc ggatggacct gttatgcaga agaagactat gggatgggaa gcttcaagtg 4081 aaagaatgta ccctgaagac ggtgctctta agggagagat taaacaacgt cttaaattga 4141 aagatggagg acattacgat gctgaggtaa gtttaaacat gattttacta actaactaat 4201 ctgatttaaa ttttcaggtg aagacaactt acaaagccaa aaaaccagtt cagctgccag 4261 gagcgtacaa tgttaatatt aaactggata tcacctccca caacgaggat tacactatcg 4321 ttgagcaata tgaaagagct gaagggcggc actcgacagg tggcatggat gaattgtata 4381 agtagtaccc agctttcttg tacaaagtgg gtgatatctg agctccgcat cggccgctgt 4441 catcagatcg ccatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat 4501 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt 4561 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag 4621 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca 4681 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt 4741 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat 4801 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg 4861 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag 4921 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt 4981 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca 5041 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa 5101 tttgaaagtg //