LOCUS Exported 5184 bp ds-DNA circular SYN 25-OCT-2021 DEFINITION pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags. ACCESSION . VERSION . KEYWORDS pENTR4 CDS A. thaliana PARP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5184) TITLE pENTR plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1) without stop codon for C-terminal epitope tagging JOURNAL Unpublished REFERENCE 2 (bases 1 to 5184) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5184 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 31..58 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind 66..89 /label=pENTR-F /note="pENTR vectors, forward primer" protein_bind 108..207 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(3163..3262) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(3374..3393) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 3385..4194 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(3452..3471) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 4284..4872 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4773..4792 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 5037..5123 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat ggcctttttg 61 cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa ataatgattt 121 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc ttttttataa 181 tgccaacttt gtacaaaaaa gcaggctcca ccatggcaag cccacataag ccgtggaggg 241 cggagtatgc aaagtcgtcg aggtcttcat gtaaaacttg caagtccgtc attaacaagg 301 agaactttcg tcttggaaag ttggttcaat ctactcactt cgatggcatc atgcccatgt 361 ggaaccatgc ttcttgtata ctaaagaaga cgaagcagat aaaatcagtt gatgatgttg 421 aaggcataga atcacttcgt tgggaagatc agcaaaagat tagaaaatat gtcgaatctg 481 gagcagggag taacacaagc acaagcacag gcacaagcac gagcagtacc gctaataatg 541 ctaaactaga atatgggatt gaagtgtcac aaacttctcg tgccggttgc agaaagtgta 601 gcgaaaagat cttgaaagga gaggtacgta tattctccaa gcctgaaggc ccgggtaaca 661 aaggtttgat gtggcatcac gctaaatgtt tccttgaaat gtcttcctct actgaactgg 721 aaagtttgtc tggatggaga agtataccag actcagacca agaagctctt cttcccttag 781 tgaagaaagc tctgccggca gccaaaactg agacagcaga agcacgtcaa acaaattcaa 841 gagcaggcac aaaacgaaaa aatgattctg ttgataacga gaagtcgaaa ctagcaaaaa 901 gtagttttga catgtctaca agtggtgctt tacaaccttg tagcaaagaa aaggaaatgg 961 aggcccaaac taaggaattg tgggacctga aggatgatct gaaaaaatat gtaacatcag 1021 ctgagttgcg ggaaatgctt gaagtaaatg aacaaagtac aagaggatct gaacttgatc 1081 tgcgtgataa atgtgctgat ggcatgatgt ttggcccact cgctctctgc ccaatgtgct 1141 ctgggcatct ttctttctcc ggaggacttt accgatgcca tggatacatc tcagaatgga 1201 gcaaatgttc tcattccact ttggatccag accgcatcaa agggaagtgg aaaatccctg 1261 acgaaacaga aaatcaattc cttctgaagt ggaataagtc tcaaaagagt gtgaagccaa 1321 aacgtattct gcgtcctgta ttgtctggcg agacatctca gggtcaaggt tctaaagatg 1381 caactgactc ctcaaggagt gaaaggctag cagatcttaa agtttcaatt gctggaaata 1441 ctaaggaaag gcaaccatgg aagaagagaa ttgaggaagc tggtgcagag tttcatgcta 1501 atgttaaaaa aggtacaagc tgtttggttg tttgtggact gacagatatc agagacgctg 1561 aaatgagaaa ggcaaggagg atgaaagtgg caatcgtgag agaggattat ttggttgatt 1621 gttttaaaaa acagaggaaa cttccatttg acaagtacaa aattgaagac actagtgaga 1681 gccttgtcac tgttaaagta aaaggacgaa gcgctgtgca tgaagcgtct ggcctccaag 1741 agcactgtca cattcttgaa gatgggaaca gtatctataa cacaactctg agcatgtctg 1801 atctctctac cggtatcaat agttattaca tactccagat aatccaagaa gataaaggtt 1861 cagattgtta cgtatttcgt aaatggggcc gagttggaaa tgaaaagatt ggtggtaaca 1921 aagtggagga aatgtcaaag tctgatgcgg ttcacgaatt caaacgtcta tttcttgaaa 1981 aaaccggaaa cacatgggaa tcttgggaac aaaaaacgaa tttccagaaa caacctggaa 2041 aatttctccc gttggacatt gattatggag ttaataagca agtagccaaa aaagagccat 2101 ttcagaccag tagcaacctt gctccatcat taatagaatt gatgaagatg ctttttgatg 2161 tggaaactta cagatctgca atgatggagt tcgagataaa tatgtcagag atgccacttg 2221 ggaagctcag caaacataat atacagaagg gttttgaggc attgacggag atacagaggc 2281 tattgactga aagcgacccc cagcctacta tgaaagaaag cttgcttgtt gatgctagta 2341 acagattttt taccatgatc ccttctattc atcctcatat tatccgagat gaagatgact 2401 ttaagtcaaa ggtgaaaatg ctcgaggctc tgcaggatat cgaaatagct tcaagaatag 2461 ttggctttga tgttgatagc accgaatctc ttgatgataa gtataagaag ctgcattgcg 2521 atatctcacc acttcctcat gatagcgaag attatcgatt aatcgagaag tatcttaaca 2581 caactcatgc cccaacgcat acagagtgga gtcttgagct agaggaagtt tttgctcttg 2641 aaagagaagg agagtttgat aaatatgctc cccacagaga aaaacttggc aataagatgc 2701 tcctatggca tggttctcga ttaacgaatt ttgttggaat attgaaccaa ggactgagaa 2761 ttgcacctcc agaagctcct gctactggtt acatgtttgg aaaagggata tactttgctg 2821 accttgtcag taaaagtgct cagtactgct acacttgtaa gaaaaatccg gtgggtctaa 2881 tgcttctgag tgaagttgca ttgggagaaa tacatgagct aacaaaagct aagtatatgg 2941 ataaacctcc gagagggaaa cactcgacca aagggctcgg caagaaagtg cctcaagatt 3001 ccgagtttgc caagtggaga ggtgatgtga ctgttccctg tggaaaacct gtttcatcaa 3061 aggtcaaggc ttctgagctt atgtacaatg agtatatcgt ctacgataca gcccaggtga 3121 agttgcagtt cttgttgaaa gtaaggttta agcacaagag agacccagct ttcttgtaca 3181 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca 3241 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc 3301 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac 3361 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga 3421 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 3481 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg 3541 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 3601 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tggtgatgca 3661 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct 3721 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 3781 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 3841 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 3901 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc 3961 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 4021 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 4081 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 4141 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa 4201 ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc gtcagacccc 4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 4921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct 4981 agcatggatc tcggggacgt ctaactacta agcgagagta gggaactgcc aggcatcaaa 5041 taaaacgaaa ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga 5101 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc 5161 ccggagggtg gcgggcagga cgcc //