LOCUS       Exported               12749 bp ds-DNA     circular SYN 25-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pimEJ5GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Bennardo N, Cheng A, Huang N, Stark JM
  TITLE     Alternative-NHEJ is a mechanistically distinct pathway of mammalian 
            chromosome break repair.
  JOURNAL   PLoS Genet. 2008 Jun 27;4(6):e1000110. doi: 
            10.1371/journal.pgen.1000110.
  PUBMED    18584027
REFERENCE   2  (bases 1 to 12749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             557..1579
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     enhancer        1733..2112
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2391..3407
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3331..3353
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3415..3434
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3489..3986
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3524..3547)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3919..3938
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4005..4604
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4005..4024)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4501..4521
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      5269..5278
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5275..5994
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5275..5991
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5320..5341)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5581..5600)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5928..5949
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6199..6218)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6264..6319
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6318..6337)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(10404..10422)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10405..10422)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10503..10522)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10622..10644
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10682..10700)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10768..10872
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10873..11733
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11091..11110)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11904..12492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12393..12412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12646..12663
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccggcag atctgatccg ctcgagcgga tcatcgccgg
       61 gtgagtgtcc ccaccccacc ctgggcgcgc gggtcgttgg gacgccggga gacccccgcg
      121 tgggtcgagc gcgctgagtg tcccgtgcct ctttcctagg caaagagaag gagcccctgg
      181 agtcgcagta ccaggtgggc ccgctgttgg gcagcggtgg cttcggctcg gtctactctg
      241 gcatccgcgt cgccgacaac ttgccggtga gtgggcaacg ccgcctcggg gagggcgcgc
      301 ggagggatgc tttgagcagg agggtctgat ggagactcgg gggtcttctt ccaggtggcc
      361 attaagcacg tggagaagga ccggatttcc gattggggag aactggtgag tgagccggag
      421 ccagagaccc tctcatgggt gggtgggtgg cgacgggcgt gccggaaccg gcgcgctaac
      481 cctcgccctc actctgcagc ccaatggcac ccgagtgccc atggaagtgg tcctgttgaa
      541 gaaggtgagc tcgctcaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
      601 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
      661 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
      721 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
      781 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
      841 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
      901 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
      961 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     1021 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     1081 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     1141 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     1201 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     1261 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     1321 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     1381 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     1441 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     1501 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     1561 tccgagggca aaggaatagt cgactacacg gactttgatg gtgagccagg ccccattatt
     1621 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1681 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     1741 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     1801 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     1861 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     1921 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     1981 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2041 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2101 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     2161 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     2221 gggggggggg gggnsgcgcg ccrggsgggg sggggsgggg sgrggggsgg ggsggggsga
     2281 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
     2341 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
     2401 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
     2461 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
     2521 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     2581 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     2641 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     2701 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2761 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     2821 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     2881 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     2941 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3001 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3061 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3121 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3181 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3241 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     3301 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3361 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3421 tgctggttat tgtgctgtct catcattttg gcaaagaatt ctagggataa cagggtaata
     3481 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     3541 cccgctgggc acttggcgct acacaagtgg cctctggcct cacacattcc acatccaccg
     3601 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     3661 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     3721 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     3781 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     3841 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     3901 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     3961 cctcttcctc atctccgggc ctttcgaccg atccagccgc caccatgacc gagtacaagc
     4021 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     4081 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     4141 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     4201 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     4261 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     4321 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     4381 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     4441 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     4501 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     4561 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     4621 cgcagcgccc gaccgaaagg agcgcacgac cccatggctc cgaccgaagc cacccggggc
     4681 ggccccgccg accccgcacc cgcccccgag gcccaccgcg ggggacacac cgaacacgcc
     4741 gaccctgctg aacacgcggc gcagttcggt gcccaggagc ggatcgaaat tgatgatcta
     4801 ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt gttaagaagg
     4861 gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg tgggggtggg
     4921 gtgggattag ataaatgcct gctctttact gaaggctctt tactattgct ttatgataat
     4981 gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc
     5041 ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt ttctcttgat
     5101 tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat agcctgaaga
     5161 acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt tgccaagttc
     5221 taattccatc agaagcttag ggataacagg gtaatggatc caccggtcgc caccatggtg
     5281 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5341 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5401 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5461 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     5521 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5581 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5641 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5701 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5761 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5821 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5881 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5941 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     6001 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     6061 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaatt
     6121 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     6181 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     6241 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     6301 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     6361 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     6421 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     6481 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     6541 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     6601 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     6661 caagctggcg tcgactacac ggactttgat ggtgagccag gccagcggcc gctagcggcc
     6721 gctgggagga gctgcccaag gtgccggcct ggaagcctct ggcggcctcg gctccggccc
     6781 tttgtaaagg tcattgggcc gcctggctca gagctggcag gggtggggca gaggcgagct
     6841 tcccggcata gtacagccgg ggatttcgag ctggttgaac gaacctgcaa catttctggc
     6901 gtgattgggg ttttgttgtt gttgttgttg tttttgttgt tgctgctggt gtttgcttgt
     6961 ttttcttttc agcaggaatt tattaaattc catgctttcc cggtgaataa gaggtcgtgg
     7021 gtagccggct gtagcccaga tttctaaagt atgacccact tttccctcca gtaggaggtg
     7081 gagccgttgg tggcgggaga cattggtgct gatttttttc tttttctttt tctttttttt
     7141 ttttttttct tttgtagttt tgttttgtgg gggagtttta aggtagttct tagaactgca
     7201 gggttttgtt ttgttttgtt ttgcttttga gggtaaagga ataagagccg cggtttcacc
     7261 ccacttgggg gtgttgtggg gtgaggaggc ctggaatgaa tgttgtgaaa taagatctgt
     7321 tgctggtttg aaaattagtt gggagtctcc gctgggagga tgaaaaccta tctaccatag
     7381 ggaggggctt ggatcgcgtt ctttcagcag tggagggcag gaatagtaga tcaaagcgcg
     7441 agggtgggtc acgatccgag tggctcaccc ggacaaacta tggcctttca aaacttgtga
     7501 ctctggcttg tggtttgaat ttctttggag gatgccgggc tgggctgcgt tttttgtatt
     7561 taacagttaa tggctggctg agtcctcccc tgtgttttcc ccccctgggc aagtctttgc
     7621 tgccccctgt tgagcaccac gaggctagca cctttttttt tttttttttt ttttactccc
     7681 caaagtttgt aaacaggaat cacgtggtct gaaaccagcc ctgcaactgt acctttccag
     7741 tctagggagt gggagagtgc agtgtctatg ccttgcttgc atagaacccg gaggaaagca
     7801 caccctgagt tagttgtgta taaaggccct tacctgggcc cggtctagat gagactggtg
     7861 tggcagggcc ctggggtcct tgtagttaaa ggctaaccca ctgagtgggg cctctagact
     7921 tgagcttttc ggtggcttta tttgtctttg ggccgatgtg taagggattt gggtttaggg
     7981 tttttgggct cccagagagc taccatatca tttcttcatt ttctgtctgg accagagagg
     8041 cagagatgga gagaaaaaaa aatatggttg ttttaaagtc aaatctgcct gtctgcctcc
     8101 tgagagctgg gattaaaggc atgtgccacc aaacccgagc caggcagtgt ctttattaaa
     8161 aaatatttga aaacctagtg gaggtggtgg ttcttgcttt taatccagcc cttgggaggt
     8221 agaggtaggc agatctccga gttcaaggcc agcctggcct acagagtcac ttccagagct
     8281 acacagagaa actttggagg agaaaaaaaa aattaaaaga agcggctcag actagctgct
     8341 ttgttaactt tttttttgct aatgctggtt tctttctatt ccctctgctg gtagggaccc
     8401 gagtgtacag tcctccagag tggattcgct accatcgcta ccacggcagg tcggcagctg
     8461 tctggtccct tgggatcctg ctctatgaca tggtctgcgg agatattccg tttgagcacg
     8521 atgaagagat catcaagggc caagtgttct tcaggcaaac tgtctcttca ggtaacttcc
     8581 ctcagacacc cccaatcctt cccaaaccct ggttcagggt tggtttgggg attacaagag
     8641 tgagagagta ttcctatcca cctttttatt tttatttatt cattttcttt gggggagagt
     8701 aaggccttag cctgcctctg acccccaact gctgggactc aagagatggt gggttttcac
     8761 tgggtcagag tactccattt gggctcaaat tggaacaagt tttatataca tcccacccca
     8821 acccccagac cctgtaaaat ctaagtacca gcagagaccc agtctctaaa caaagtttaa
     8881 aaaaaaaaaa aaaaaatgaa ggttccggat agtgtcagtg gaaggcgtga ggtaggtagg
     8941 tgaattgaat cacctcatct tgctcactgg tggctcatca aaacccttgt catccttcct
     9001 atttctagtg agtgggtgtc atgggaaagg cccgagctat tgaagtctga aaccacaggt
     9061 ttaagagggg aggcactttt ttaactgaaa gctgattgct ggaggctttc ggtatccgtt
     9121 ctttcccacc gggcagtgtt tggtataaaa agtaccgtgc ctctttaaaa accccagact
     9181 tgtaggtctg gggaagaaga cctttgcatt acaaaaacaa gttgagtcat ctttaacctc
     9241 tgtctgcccc gccttctgca gagtgtcagc accttattaa atggtgcctg tccctgagac
     9301 cgtcagatcg gccctccttt gaagaaatcc ggaaccatcc gtggatgcag ggtgacctcc
     9361 tgccccaggc agcttctgag atccatctgc acagtctgtc accgggatcc agcaagtagc
     9421 agcctttctg ctgctgtctg tcagaccctc cagggaggga gagcttgtct tctggcctcc
     9481 aacaggaccc tgcgtgacga tgcagggaca gcaatgacaa ctcattccag actccaggtc
     9541 cctggaggag cctcccacaa gggaaagaga ctacttcact ggtcctgggc ccctctttgc
     9601 gcgccccgcc cccagactca gcgtctagtg ttgctgggct cccctgaatc catccctggt
     9661 tgggggtggg agtggatcag agccctgtcg atggaacttt agtcaccatg gagactgggt
     9721 caccaagatg ggccgggggg gagaggaaaa cagtctgggg ggtgggataa aaactagcac
     9781 catatttaag tccctgtcac ctcttccaac tcctgagtgc cttctgtggg gactccgcct
     9841 gtgctgggag aaatacttga actttacctc ttacctgctg cttctcaaac tctgcctggg
     9901 ttttgcttcc ttcctctcct ctcctttccc atcccccact tcctatgaaa ggtgccatgg
     9961 gagaggctac agggccaact gctgagccac ctgccccttt tctgtctcct ttagtaaaat
    10021 tcccaagcaa actggtcttc ctctttggtt tcgacttaac tgtttcaaaa ccaagacctc
    10081 acacagaacg cacaaccaat gcaaaacaaa aaacaaaaaa aacaaaaaaa aaaccagaca
    10141 agctgtaaat gtgtgtatag ttccggcatg gtagcctgca aaaggactgt agatgataac
    10201 aattttaaag aagaaaaaaa aatgccttta agttatttta tgtgttgggg gtttggtttg
    10261 gttttttgtt tttgtttttt tttttctttt ttattctttt ttgcttgttt ttgaaagatg
    10321 gcacgttcta gcccggaagt tttaatgtat ttatttattt atttatttgg ttgccttcct
    10381 gtccaagctt cagctgctcg agttctatag tgtcacctaa atcgtatgtg tatgatacat
    10441 aaggttatgt attaattgta gccgcgttct aacgacaata tgtccatatg gtgcactctc
    10501 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
    10561 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    10621 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
    10681 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10741 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10801 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10861 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10921 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10981 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    11041 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    11101 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    11161 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    11221 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    11281 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    11341 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    11401 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    11461 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    11521 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    11581 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    11641 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    11701 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    11761 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11821 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11941 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    12001 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    12061 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    12121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    12181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    12241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    12301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    12361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    12421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    12481 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    12541 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    12601 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    12661 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    12721 taatgcaggt taacctggct tatcgaaat
//
