LOCUS Exported 5713 bp ds-DNA circular SYN 29-OCT-2021 DEFINITION Constitutively active, nucelar localized RhoJ. ACCESSION . VERSION . KEYWORDS H2A-mTurquoise-RhoJQ79L-deltaCaax SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5713) TITLE Gadella lab plasmids REFERENCE 2 (bases 1 to 5713) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5713 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 413..422 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 419..1135 /codon_start=1 /product="enhanced monomeric variant of CFP (Goedhart et al., 2010)" /label=mTurquoise /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(464..485) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(725..744) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1072..1093 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1872..1993 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1909..1928) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1963..1982 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2000..2455) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2137..2158) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2349..2368 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2482..2586 /gene="bla" /label=AmpR promoter promoter 2588..2945 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2611..2631) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2796..2931 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2858..2877 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2980..3774 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3034..3053) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3644..3663 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3962..3981) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4006..4053 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4382..4970 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4871..4890 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 5145..5448 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5449..5652 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5603..5623 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" ORIGIN 1 aattctgatg tcgggacgcg gcaagcaggg aggcaaagct cgcgccaaag ccaagacccg 61 ctcttctcgt gccggtctcc agttccccgt gggccgagtg caccgactgc tccgcaaggg 121 caactatgct gagcgggtcg gggccggcgc gccggtgtac ctggcggcgg tgctggagta 181 cctgactgcc gagatcctgg agctggcggg caacgccgcc cgcgacaaca agaagacccg 241 cattatcccg cgccacttgc agctggccat ccgcaacgac gaggagctca acaagctgct 301 gggcaaagta accatcgctc agggtggtgt cctgcccaac atccaggctg tgctactgcc 361 caagaagacc gagagtcacc acaaggccaa aggcaaacgg gatccaccgg tcgccaccat 421 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 481 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 541 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 601 cgtgaccacc ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca 661 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 721 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 781 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 841 gctggagtac aactacatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg 901 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga 961 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 1021 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 1081 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccat 1141 gaactgcaaa gagggaactg acagcagctg cggctgcagg ggcaacgacg agaagaagat 1201 gttgaagtgt gtggtggtgg gggacggtgc cgtggggaaa acctgcctgc tgatgagcta 1261 cgccaacgac gccttcccag aggaatacgt gcccactgtg tttgaccact atgcagttac 1321 tgtgactgtg ggaggcaagc aacacttgct cggactgtat gacaccgcgg gactggagga 1381 ctacaaccag ctgaggccac tctcctaccc caacacggat gtgtttttga tctgcttctc 1441 tgtcgtaaac cctgcctctt accacaatgt ccaggaggaa tgggtccccg agctcaagga 1501 ctgcatgcct cacgtgcctt atgtcctcat agggacccag attgatctcc gtgatgaccc 1561 aaaaaccttg gcccgtttgc tgtatatgaa agagaaacct ctcacttacg agcatggtgt 1621 gaagctcgca aaagcgatcg gagcacagtg ctacttggaa tgttcagctc tgactcagaa 1681 aggtctcaaa gcggtttttg atgaagcaat cctcaccatt ttccacccca agaaaaagaa 1741 gaaacgctaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt 1801 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 1861 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 1921 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 1981 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 2041 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 2101 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 2161 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 2221 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 2281 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 2341 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 2401 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt 2461 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 2521 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 2581 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt 2641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2701 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2761 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 2821 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 2881 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 2941 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 3001 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 3061 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 3121 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta 3181 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 3241 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 3301 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 3361 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 3421 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 3481 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc 3541 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 3601 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 3661 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 3721 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 3781 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 3841 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 3901 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa 3961 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac 4021 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct 4081 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct 4141 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg 4201 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc 4261 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 4321 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 4381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 4441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 4501 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 4561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 4621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 4681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 4741 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 4921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 4981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 5041 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa 5101 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 5161 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 5221 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 5281 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 5341 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 5401 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 5461 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 5521 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 5581 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 5641 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg 5701 agctcaagct tcg //