LOCUS Exported 8543 bp ds-DNA circular SYN 05-NOV-2021 DEFINITION non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system. ACCESSION . VERSION . KEYWORDS pUCmini-iCAP-AAV.CAP-B10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8543) TITLE Brain-wide gene expression with decreased liver targeting after intravenous delivery of engineered AAV capsid in mouse and marmoset JOURNAL Unpublished REFERENCE 2 (bases 1 to 8543) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 5, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8543 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 130..152 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 144..161 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 145..161 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 171..190 /label=T7 /note="T7 promoter, forward primer" promoter 171..189 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2779..2889 /codon_start=1 /product="3 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP48 /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML" promoter complement(2928..2946) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2929..2946) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2966..2983) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2972..3196 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" protein_bind 3258..3276 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3295..3313 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3332..3350 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3369..3387 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3406..3424 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3443..3461 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3480..3498 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 3517..3535 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" CDS 6252..6263 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" rep_origin complement(6819..7407) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6899..6918) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7578..8438) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8201..8220 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8439..8543) /gene="bla" /label=AmpR promoter ORIGIN 1 cacatttccc cgaaaagtgc cacctgacgt cccaatgata cgcgtcggtg cgggcctctt 61 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 121 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact 181 cactataggg cgaattgggt acatcgacgg tatcggggga gctcgcaggg tctccatttt 241 gaagcgggag gtttgaacgc gcagccgcca tgccggggtt ttacgagatt gtgattaagg 301 tccccagcga ccttgacgag catctgcccg gcatttctga cagctttgtg aactgggtgg 361 ccgagaagga atgggagttg ccgccagatt ctgacatgga tctgaatctg attgagcagg 421 cacccctgac cgtggccgag aagctgcagc gcgactttct gacggaatgg cgccgtgtga 481 gtaaggcccc ggaggctctt ttctttgtgc aatttgagaa gggagagagc tacttccaca 541 tgcacgtgct cgtggaaacc accggggtga aatccatggt tttgggacgt ttcctgagtc 601 agattcgcga aaaactgatt cagagaattt accgcgggat cgagccgact ttgccaaact 661 ggttcgcggt cacaaagacc agaaatggcg ccggaggcgg gaacaaggtg gtggatgagt 721 gctacatccc caattacttg ctccccaaaa cccagcctga gctccagtgg gcgtggacta 781 atatggaaca gtatttaagc gcctgtttga atctcacgga gcgtaaacgg ttggtggcgc 841 agcatctgac gcacgtgtcg cagacgcagg agcagaacaa agagaatcag aatcccaatt 901 ctgatgcgcc ggtgatcaga tcaaaaactt cagccaggta catggagctg gtcgggtggc 961 tcgtggacaa ggggattacc tcggagaagc agtggatcca ggaggaccag gcctcataca 1021 tctccttcaa tgcggcctcc aactcgcggt cccaaatcaa ggctgccttg gacaatgcgg 1081 gaaagattat gagcctgact aaaaccgccc ccgactacct ggtgggccag cagcccgtgg 1141 aggacatttc cagcaatcgg atttataaaa ttttggaact aaacgggtac gatccccaat 1201 atgcggcttc cgtctttctg ggatgggcca cgaaaaagtt cggcaagagg aacaccatct 1261 ggctgtttgg gcctgcaact accgggaaga ccaacatcgc ggaggccata gcccacactg 1321 tgcccttcta cgggtgcgta aactggacca atgagaactt tcccttcaac gactgtgtgg 1381 acaagatggt gatctggtgg gaggagggga agatgaccgc caaggtcgtg gagtcggcca 1441 aagccattct cggaggaagc aaggtgcgcg tggaccagaa atgcaagtcc tcggcccaga 1501 tagacccgac tcccgtgatc gtcacctcca acaccaatat gtgcgccgtg attgacggga 1561 actcaacgac cttcgaacac cagcagccgt tgcaagaccg gatgttcaaa tttgaactca 1621 cccgccgtct ggatcatgac tttgggaagg tcaccaagca ggaagtcaaa gactttttcc 1681 ggtgggcaaa ggatcacgtg gttgaggtgg agcatgaatt ctacgtcaaa aagggtggag 1741 ccaagaaaag acccgccccc agtgacgcag atataagtga gcccaaacgg gtgcgcgagt 1801 cagttgcgca gccatcgacg tcagacgcgg aagcttcgat caactacgcg gacaggtacc 1861 aaaacaaatg ttctcgtcac gtgggcatga atctgatgct gtttccctgt agacaatgcg 1921 agagactgaa tcagaattca aatatctgct tcactcacgg tgtcaaagac tgtttagagt 1981 gctttcccgt gtcagaatct caacccgttt ctgtcgtcaa aaaggcgtat cagaaactgt 2041 gctacattca tcacatcatg ggaaaggtgc cagacgcttg cactgcttgc gacctggtca 2101 atgtggactt ggatgactgt gtttctgaac aataaatgac ttaaaccagg tatgagtcgg 2161 ctggataaat ctaaagtcat aaacggcgct ctggaattac tcaatgaagt cggtatcgaa 2221 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg 2281 cacgtgaaga acaagcgggc cctgctcgat gccctggcca tcgagatgct ggacaggcat 2341 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc 2401 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc 2461 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa 2521 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc 2581 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc 2641 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa 2701 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc 2761 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat 2821 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt 2881 gacatgctcc ccgggtaaat gcatgaattc gatctagagg gccctattct atagtgtcac 2941 ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 3001 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 3061 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 3121 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 3181 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgaa tcaagctatc 3241 aagtgccacc tgacgtctcc ctatcagtga tagagaagtc gacacgtctc gagctcccta 3301 tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga agtcgacacg 3361 tctcgagctc cctatcagtg atagagaagg tacgtctaga acgtctccct atcagtgata 3421 gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc tagaacgtct 3481 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta 3541 ccccctatat aagcagagag atctgttcaa atttgaactg actaagcggc tcccgccaga 3601 ttttggcaag attactaagc aggaagtcaa ggactttttt gcttgggcaa aggtcaatca 3661 ggtgccggtg actcacgagt ttaaagttcc cagggaattg gcgggaacta aaggggcgga 3721 gaaatctcta aaacgcccac tgggtgacgt caccaatact agctataaaa gtctggagaa 3781 gcgggccagg ctctcatttg ttcccgagac gcctcgcagt tcagacgtga ctgttgatcc 3841 cgctcctctg cgaccgctag cttcgatcaa ctacgcagac aggtaccaaa acaagtgttc 3901 tcgtcacgtg ggcattaatc tgattctgtt tccctgcaga caatgcgaga gaatgaatca 3961 gaactcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc 4021 agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca 4081 tatcatggga aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg tggatttgga 4141 tgactgcatc tttgaacaat aaatgactta agccaggtat ggctgccgat ggttatcttc 4201 cagattggct cgaggacaac cttagtgaag gaattcgcga gtggtgggct ttgaaacctg 4261 gagcccctca acccaaggca aatcaacaac atcaagacaa cgctagaggt cttgtgcttc 4321 cgggttacaa ataccttgga cccggcaacg gactcgacaa gggggagccg gtcaacgcag 4381 cagacgcggc ggccctcgag cacgacaaag cctacgacca gcagctcaag gccggagaca 4441 acccgtacct caagtacaac cacgccgacg ccgagttcca ggagcggctc aaagaagata 4501 cgtcttttgg gggcaacctc gggcgagcag tcttccaggc caaaaagagg cttcttgaac 4561 ctcttggtct ggttgaggaa gcggctaaga cggctcctgg aaagaagagg cctgtagagc 4621 agtctcctca ggaaccggac tcctccgcgg gtattggcaa atcgggtgca cagcccgcta 4681 aaaagagact caatttcggt cagactggcg acacagagtc agtcccagac cctcaaccaa 4741 tcggagaacc tcccgcagcc ccctcaggtg tgggatctct tacaatggct tcaggtggtg 4801 gcgcaccagt ggcagacaat aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt 4861 ggcattgcga ttcccaatgg ctgggggaca gagtcatcac caccagcacc cgaacctggg 4921 ccctgcccac ctacaacaat cacctctaca agcaaatctc caacagcaca tctggaggat 4981 cttcaaatga caacgcctac ttcggctaca gcaccccctg ggggtatttt gacttcaaca 5041 gattccactg ccacttctca ccacgtgact ggcagcgact catcaacaac aactggggat 5101 tccggcctaa gcgactcaac ttcaagctct ttaacattca ggtcaaagag gttacggaca 5161 acaatggagt caagaccatc gccaataacc ttaccagcac ggtccaggtc ttcacggact 5221 cagactatca gctcccgtac gtgctcgggt cggctcacga gggctgcctc ccgccgttcc 5281 cagcggacgt tttcatgatt cctcagtacg ggtatctgac gcttaatgat ggaagccagg 5341 ccgtgggtcg ttcgtccttt tactgcctgg aatatttccc gtcgcaaatg ctaagaacgg 5401 gtaacaactt ccagttcagc tacgagtttg agaacgtacc tttccatagc agctacgctc 5461 acagccaaag cctggaccga ctaatgaatc cactcatcga ccaatacttg tactatctct 5521 ctagaactat tgacggcgcc gccaccaaaa accaaacgct aaaattcagt gtggccggac 5581 ccagcaacat ggctgtccag ggaagaaact acatacctgg acccagctac cgacaacaac 5641 gtgtctcaac cactgtgact caaaacaaca acagcgaatt tgcttggcct ggagcttctt 5701 cttgggctct caatggacgt aatagcttga tgaatcctgg acctgctatg gcctctcaca 5761 aagaaggaga ggaccgtttc tttcctttgt ctggatcttt aatttttggc aaacaaggta 5821 ctggcagaga caacgtggat gcggacaaag tcatgataac caacgaagaa gaaattaaaa 5881 ctactaaccc ggtagcaacg gagtcctatg gacaagtggc cacaaaccac cagagtgatg 5941 ggactttggc ggtgcctttt aaggcacagg cgcagaccgg ttgggttcaa aaccaaggaa 6001 tacttccggg tatggtttgg caggacagag atgtgtacct gcaaggaccc atttgggcca 6061 aaattcctca cacggacggc aactttcacc cttctccgct gatgggaggg tttggaatga 6121 agcacccgcc tcctcagatc ctcatcaaaa acacacctgt acctgcggat cctccaacgg 6181 ccttcaacaa ggacaagctg aactctttca tcacccagta ttctactggt caagtcagcg 6241 tggagatcga gtgggagctg cagaaggaaa acagcaagcg ctggaacccg gagatccagt 6301 acacttccaa ctattacaag tctaataatg ttgaatttgc tgttaatact gaaggtgtat 6361 atagtgaacc ccgccccatt ggcaccagat acctgactcg taatctgtaa gtcgacttgc 6421 ttgttaatca ataaaccgtt taattcgttt cagttgaact ttggtctctg cgaagggcaa 6481 ttcgtttaaa cctgcaggac tagaggtcct gtattagagg tcacgtgagt gttttgcgac 6541 attttgcgac accatgtggt cacgctgggt atttaagccc gagtgagcac gcagggtctc 6601 cattttgaag cgggaggttt gaacgcgcag ccgccaagcc gaattctgca gatatcacat 6661 gtcctaggaa ctatcgatcc atcacactgg cggccgctcg actagagcgg ccgccaccgc 6721 ggtggagctc cagcttttgc ggaccgaatc ggaaagaaca tgtgagcaaa aggccagcaa 6781 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 6841 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 6901 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 6961 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7021 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7081 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7141 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7201 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 7261 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7321 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7381 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7681 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 7861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 7921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 7981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8521 aataaacaaa taggggttcc gcg //