LOCUS Exported 6816 bp ds-DNA circular SYN 12-NOV-2021 DEFINITION Localization: Membrane Associated Kinase, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS tdEos-FYN-N-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6816) TITLE Davidson Photoactivatable Fluorescent Proteins REFERENCE 2 (bases 1 to 6816) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6816 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 537..546 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2019..2696 /codon_start=1 /product="green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)" /label=EosFP /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" CDS 2745..3422 /codon_start=1 /product="green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)" /label=EosFP /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN ARR" polyA_signal 3544..3665 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3581..3600) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3635..3654 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3672..4127) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3809..3830) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4021..4040 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4154..4258 /gene="bla" /label=AmpR promoter promoter 4260..4617 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4283..4303) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4468..4603 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4530..4549 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4652..5446 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4706..4725) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5316..5335 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5634..5653) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5678..5725 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6054..6642 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6543..6562 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 541 ccatgggctg tgtgcaatgt aaggataaag aagcaacaaa actgacggag gagagggacg 601 gcagcctgaa ccagagctct gggtaccgct atggcacaga ccccacccct cagcactacc 661 ccagcttcgg tgtgacctcc atccccaact acaacaactt ccacgcagcc gggggccaag 721 gactcaccgt ctttggaggt gtgaactctt cgtctcatac ggggaccttg cgtacgagag 781 gaggaacagg agtgacactc tttgtggccc tttatgacta tgaagcacgg acagaagatg 841 acctgagttt tcacaaagga gaaaaatttc aaatattgaa cagctcggaa ggagattggt 901 gggaagcccg ctccttgaca actggagaga caggttacat tcccagcaat tatgtggctc 961 cagttgactc tatccaggca gaagagtggt actttggaaa acttggccga aaagatgctg 1021 agcgacagct attgtccttt ggaaacccaa gaggtacctt tcttatccgc gagagtgaaa 1081 ccaccaaagg tgcctattca ctttctatcc gtgattggga tgatatgaaa ggagaccatg 1141 tcaaacatta taaaattcgc aaacttgaca atggtggata ctacattacc acccgggccc 1201 agtttgaaac acttcagcag cttgtacaac attactcagg tacctggaat ggaaacacaa 1261 aagtagccat aaagactctt aaaccaggca caatgtcccc cgaatcattc cttgaggaag 1321 cgcagatcat gaagaagctg aagcacgaca agctggtcca gctctatgca gtggtgtctg 1381 aggagcccat ctacatcgtc accgagtata tgaacaaagg aagtttactg gatttcttaa 1441 aagatggaga aggaagagct ctgaaattac caaatcttgt ggacatggca gcacaggtgg 1501 ctgcaggaat ggcttacatc gagcgcatga attatatcca tagagatctg cgatcagcaa 1561 acattctagt ggggaatgga ctcatatgca agattgctga cttcggattg gcccgattga 1621 tagaagacaa tgagtacaca gcaagacaag gtgcaaagtt ccccatcaag tggacggccc 1681 ccgaggcagc cctgtacggg aggttcacaa tcaagtctga cgtgtggtct tttggaatct 1741 tactcacaga gctggtcacc aaaggaagag tgccataccc aggcatgaac aaccgggagg 1801 tgctggagca ggtggagcga ggctacagga tgccctgccc gcaggactgc cccatctctc 1861 tgcatgagct catgatccac tgctggaaaa aggaccctga agaacgcccc acttttgagt 1921 acttgcagag cttcctggaa gactacttta ccgcgacaga gccccagtac caacctggtg 1981 aaaacctggc atccagcggg gatccaccgg tcgccaccat gagtgcgatt aagccagaca 2041 tgaagatcaa cctccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag 2101 atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg 2161 gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat 2221 tcgccgaata tccagaccac atacaagact attttaagca gtcgtttcct aaggggtatt 2281 cgtgggaacg aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa 2341 caatggaagg ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca 2401 atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg 2461 tgcgtgatgg agtgctgact ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc 2521 attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag 2581 gctaccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg 2641 ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgatccg 2701 gtagcggtca cggtactggt tctactggtt ctggttcttc tgagagtgcg attaagccag 2761 acatgaagat caacctccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg 2821 gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg 2881 gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg 2941 tattcgccga atatccagac cacatacaag actattttaa gcagtcgttt cctaaggggt 3001 attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc agaaacgaca 3061 taacaatgga aggggacact ttctataata aagttcgatt tcacggtgta aactttcccg 3121 ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt 3181 atgtgcgtga tggagtgctg actggtgata ttcgcatggc tttgttgctt gaaggaaatg 3241 cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac 3301 caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca 3361 aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat 3421 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 3481 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 3541 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3601 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3661 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 3721 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 3781 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 3841 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 3901 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 3961 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 4021 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 4081 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 4141 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 4201 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 4261 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 4321 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 4381 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 4441 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 4501 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 4561 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 4621 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 4681 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 4741 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 4801 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 4861 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 4921 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 4981 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 5041 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 5101 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 5161 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 5221 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 5281 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 5341 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 5401 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 5461 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 5521 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 5581 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 5641 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 5701 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 5761 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 5821 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 5881 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 5941 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 6001 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 6061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 6121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 6181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 6241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 6301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 6361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 6421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 6481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 6541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 6601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 6721 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac 6781 ggggtcatta gttcatagcc catatatgga gttccg //