LOCUS Exported 3618 bp ds-DNA circular SYN 30-NOV-2021 DEFINITION Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT. ACCESSION . VERSION . KEYWORDS pEPQD0CM0764 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3618) TITLE Reconstitution of monoterpene indole alkaloid biosynthesis in Nicotiana benthamiana REFERENCE 2 (bases 1 to 3618) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3618 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1246..1262) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1246..1262) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1259..1281) /label=M13/pUC Reverse /note="In lacZ gene" rep_origin complement(1434..2022) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1514..1533) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2155..2249 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2270..2929) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2844..2863 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(2930..3032) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind 3119..3141 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3133..3150 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3134..3150 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 ccttgttaag ttccttctca ctggagttgg aagctttgaa ggggcaaaat ggtccaaaca 61 cagaagaatc atttcccctg ccttcactct tgagaaacta aagtcaatgt tgccggcttt 121 tgccatatgc tatcatgaca tgttgacgaa atgggagaaa ttagcagaaa aagaaggatc 181 ccatgaagtt gatatcttcc ccacatttga tgttttgaca agtgatgtaa tttcaaaggt 241 tgcatttggt agcacatacg atgaaggagg caaaatcttc agactattga aagaactcat 301 ggatctcaca attgattgca tgagagatgt ctacatccca gggtggagct acttgccaac 361 caagaggaac aagaggatga aagaaattaa caaagaaatc acagatatgc taaggttcat 421 catcaacaag agaatgaagg ctttgaaggc tggagaacca ggtgaggatg acttgttggg 481 agtcctgttg gaatcaaaca ttcaagaaat tcagaaacaa ggaaacagga aggatggtgg 541 aatgacaatc aacgatgtga ttgaggaatg caaattgttc tactttgctg gtcaagaaac 601 caccggagtt ttgctcacat ggaccaccat tttattgagc aagcatcccg agtggcaaga 661 gcgagctagg gaagaagttc tccaagcctt tggcaagaac aagcctgaat ttgaacgctt 721 gaatcatctt aaatatgttt ctatgatctt atacgaggtt ctaaggttgt acccgccagt 781 tattgatctg accaagatta ttcacgagga cacaaagtta gggccgtaca caatccctgc 841 aggaacacaa gtaatgttgc cgacagtaat gcttcataga gagaagtcca tttggggaga 901 agatgcaatg gaattcaacc caatgagatt tgctgatgga gttgccaatg ccaccaagaa 961 caatgtcaca tatttgccat tcagttgggg acctagagtt tgcctcggcc aaaactttgc 1021 acttcttcaa gcaaaattag ggttggcaat gattttacaa cgcttcaaat ttgatgtagc 1081 tccatcctat gttcatgctc cctttaccat tcttacagtt caacctcagt ttggttctca 1141 tgttatctac aagaagcttg agagacagaa tttttaagct tcgagaccta ggctaggtgg 1201 aggctcagtg atgataagtc tgcgatggtg gatgcatgtg tcatggtcat agctgtttcc 1261 tgtgtgaaat tgttatccgc tcagagggca caatcctatt ccgcgctatc cgacaatctc 1321 caagacatta ggtggagttc agttcggcgt atggcatatg tcgctggaaa gaacatgtga 1381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 1441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 1501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 2041 tctacggggt ctgacgctct attcaacaaa gccgccgtcc cgtcaagtca gcgtaaatgg 2101 gtagggggct tcaaatcgtc cccccatacg atataagttg ttactagtgc ttggattctc 2161 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga 2221 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc 2281 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2341 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2401 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2461 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2521 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2581 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2641 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2701 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2761 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2821 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2881 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2941 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat 3001 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttgatacctg aaacaaaacc 3061 catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc agggttttcc 3121 cagtcacgac gttgtaaaac gacggccagt catgcataat ccgcacgcat ctggaataag 3181 gaagtgccat tccgcctgac ctaggtctct aatggagatg gatatggata tcattagaaa 3241 ggcaattgct gccactatct ttgcattggt tatggcttgg gcgtggagag tgttggattg 3301 ggcatggttt actcctaaga ggatcgagaa acgtctaagg cagcaaggtt ttagaggaaa 3361 tccttataga ttcttggttg gagatgttaa ggagagtgga aaaatgcatc aagaagcctt 3421 gtctaacccc atggagttcg acaacgatat tgttcctcgc ctcatgccac atattaacca 3481 caccataaaa acttacggta ggaattcatt tacatggatg ggaaggattc caaggattca 3541 tgttatggaa cctgaactta ttaaggaagt attgacccac tccagcaaat accaaaagaa 3601 ctttgatgtt cacaatcc //