LOCUS Exported 5926 bp ds-DNA circular SYN 30-NOV-2021 DEFINITION AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter. ACCESSION . VERSION . KEYWORDS AAV-Syn-H2B-jGCaMP8m-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5926) TITLE Looger Lab GCaMP8 plasmids REFERENCE 2 (bases 1 to 5926) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5926 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(117..705) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(197..216) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(876..1736) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1499..1518 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1737..1841) /gene="bla" /label=AmpR promoter rep_origin complement(1867..2322) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2004..2025) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2216..2235 /label=F1ori-R /note="F1 origin, reverse primer" repeat_region 2394..2523 /label=AAV2 ITR promoter 2605..3052 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" CDS 3086..3463 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAITKYTSAK" CDS 3479..3496 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind 3778..3799 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(3905..3926) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4166..4185) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" misc_feature 4757..5345 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4810..4830) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5228..5239) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5348..5364) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5349..5365) /label=pBluescriptKS /note="For pBluescript vector" polyA_signal 5394..5515 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5405..5424) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 5459..5478 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 5677..5806 /label=AAV2 ITR primer_bind complement(5872..5889) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 61 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 301 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 421 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 481 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 541 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 601 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 661 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 721 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 781 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 961 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 1021 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1081 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1141 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1201 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1261 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1321 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1381 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1441 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1501 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1561 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1621 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1681 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 1741 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 1801 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 1861 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 1921 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 1981 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 2041 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 2101 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 2161 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 2221 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 2281 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc 2341 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg 2401 ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc 2461 cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt 2521 ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc tctaggaaga 2581 tctctgcaga gggccctgcg tatgagtgca agtgggtttt aggaccagga tgaggcgggg 2641 tgggggtgcc tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc 2701 ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg 2761 cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca 2821 ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct 2881 tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag 2941 gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct 3001 gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agtcgaattc 3061 aagctgctag caaggatccg ccaccatgcc agagccagcg aagtctgctc ccgccccgaa 3121 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg 3181 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga 3241 caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga 3301 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc 3361 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt 3421 gtccgagggt actaaggcca tcaccaagta caccagcgct aaggacccac cggtcatgca 3481 tcatcaccat catcacacgc gtcgcaagaa gaccttcaag gaggtggcca ccgccgtgaa 3541 gatcatcggc cgcctgatgg gcctgaagat caacgtctat atcaaggccg acaagcagaa 3601 gaacggcatc aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct 3661 cgcctaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 3721 ccactacctg agcgtggagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat 3781 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 3841 gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 3901 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 3961 tgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 4021 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 4081 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat 4141 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 4201 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 4261 cggcaacatc ctggggcaca agctggagta caacctgccg gaccaactga ctgaagagca 4321 gatcgcagaa tacaaagagg ctttctccct atttgacaag gacggggatg ggacaataac 4381 aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct 4441 gcaggacatg atcaatgaag tagatgccga cggtgacggc acaatcgact tccctgagtt 4501 cctgacaatg atggcaagaa aaatgaaata cagggacacg gaagaagaaa ttagagaagc 4561 gttcggtgtg tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt 4621 gatgacaaac cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc 4681 agacatcgat ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa 4741 gtaaaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 4801 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 4861 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 4921 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 4981 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 5041 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 5101 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc 5161 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 5221 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 5281 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 5341 cccgcatcga taccgtcgac ctcgacccgg gcggccgctt cgagcagaca tgataagata 5401 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 5461 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 5521 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag 5581 caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg 5641 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg 5701 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 5761 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc attaatgaat 5821 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 5881 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagct //